TaqMan™ SNP 基因分型试剂盒,人类
TaqMan™ SNP 基因分型试剂盒,人类
Applied Biosystems™

TaqMan™ SNP 基因分型试剂盒,人类

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Applied Biosystems TaqMan SNP 基因分型分析试剂盒使用 TaqMan 5´核酸酶化学试剂扩增和检测纯化基因组 DNA 样品中的特定多态性。每项测定可对个体进行单核苷酸多态性了解更多信息
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货号数量
4351379300次反应(小,40X)
43513742400次反应(大型;80X)
43513761000次反应(中型,40X)
货号 4351379
价格(CNY)
5,588.00
Each
立刻订购
数量:
300次反应(小,40X)
Applied Biosystems TaqMan SNP 基因分型分析试剂盒使用 TaqMan 5´核酸酶化学试剂扩增和检测纯化基因组 DNA 样品中的特定多态性。每项测定可对个体进行单核苷酸多态性 (SNP) 基因分型,包括两个序列特异性引物和两个 TaqMan 小沟结合物 (MGB) 探针以及非荧光淬灭剂 (NFQ)。一个探针标记有 VIC 染料,以检测等位基因1序列;另一个探针标记有 FAM 染料来检测等位基因2序列。

我们预设计的 TaqMan SNP 基因分型人类检测试剂盒是全基因组收集的数百万种人类检测试剂盒,其中包括常见的1,000 基因组 SNP、HapMap SNP 和编码 SNP

。优势:
技术成熟可靠—此款金标准的 TaqMan 化学试剂和耐用的检测产品设计能够提供准确、可重复且可靠的结果
操作简单— 单管式规格方便易用,工作流程简单易懂,为得出可靠结果提供了一种简便途径
相关—广泛收集预先设计的人类试剂盒,可直接查看与您的研究相关的内容
经过检测—所有人类 SNP 基因分型试剂盒都经过功能测试,可以确保等位基因分型顺利完成

装运时间近似
4–北美6天;欧洲6–10天

TaqMan SNP 基因分型试剂盒仅需三个反应组分进行 PCR:纯化基因组 DNA (1–20 ng)、分析液和 TaqMan 基因分型预混液(或其他兼容的预混液)(单独出售)。

所有试剂盒的设计都是来源于生物信息学流程,在过去十年中不断通过生产和性能数据进行优化。TaqMan 检测试剂盒已被超过 40,000 份出版物引用,并且拥有超过350项专利。

我们所有的预设计 TaqMan 检测试剂盒均具备 TaqMan 试剂盒 QPCR 保证。*

推荐的预混液(单独销售):TaqMan 基因分型预混液

*适用条款和条件。有关完整详细信息,请访问 www.thermofisher.cn/taqmanguarantee。

仅供科研使用。不可用于诊断程序。
规格
3' 引物修改
5' 引物修改
描述VIC™ - MGB 探针检测等位基因 1、FAM - MGB 探针检测等位基因 2
适用于(设备)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ Absolute Q Digital PCR System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler** If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR system in order to detect and record fluorescent signals.
环保功能绿色可持续包装
内部探针修改VIC™ (5')、FAM (5')、MGB(小沟结合物)(3')
反应次数300 次反应
产品线TaqMan™
纯化方法固相萃取 (SPE)
纯度或质量等级不含 RNase 和 DNase
数量300次反应(小,40X)
样品类型
运输条件室温
种属
靶标可提供超过450万种全基因组人类测定
最大浓度40X
适用于(应用)基因分型
形式冷冻
标签或染料FAM, VIC:
Unit SizeEach
内容与储存
1 管含 40X(S 和 M 规格)或 80X(L 规格)预配制测定试剂(2 探针和 2 引物)混合物的试管。

储存于 -15 到 -25°C。

常见问题解答 (FAQ)

How do I set up a reference panel in the TaqMan Genotyper Software?

A reference panel is helpful in large studies to mark your reference samples. Please follow the directions here on how to set up a reference panel.

How do I enter the polymorphism sequence information (i.e., A, C, G, T) for my assays, and where is this info displayed in the TaqMan Genotyper Software?

The polymorphism sequence info can be entered into the software through Setup >Assays. You can import an assay information file (AIF) that contains this info for your assays (AIFs are shipped with assay orders), or manually enter this info for each assay using the edit assay feature. The polymorphism sequence info will be displayed in the assays table under allele1 base and allele2 base, in the results table in the calls column, in the cluster plot display in the x-axis and y-axis titles, and in the export files as genotypes. If no sequence information is entered for an assay, the default display for genotype calls will use the dye names, such as VIC/VIC, VIC/FAM or FAM/FAM dyes.

What is the bookmarking feature in the TaqMan Genotyper Software, and how would I use it?

Bookmarking is a unique feature in TaqMan Genotyper Software that allows you to tag a data point or well while reviewing results in a Study. For example, in reviewing a cluster plot for an assay, a data point is observed to be somewhat between clusters. You can set a bookmark for this data point to denote this well for further investigation. The bookmark persists between the Results workspace and Quality Control workspace, so you can easily identify the data point in a cluster plot, experiment plate view, or on the samples tab. Bookmarks are cleared upon exit from a Study or exit from the application.

I am getting the message: "An error has occurred. See the log file C:\ProgramFiles\Applied Biosystems\TaqMan Genotyper\config\eclipse\1363113099385.log." How can I fix this?

1.Go to the Start button, then Programs, then TaqMan Genotyper Software
2.Right-click on the program and choose “Run as Administrator”
3.If that does not work, go back to the same menu and choose “Properties”
4.Choose the “Compatibility” tab, and check “Run this program as administrator”
5.Click “Apply”
6.You may+C69 have to restart the computer for the settings to apply

Can I delete an assay or sample from my qPCR study?

An assay or sample may be deleted from a study only if there is no data or wells associated with it. Upon import of an experiment, the software collects all the assays and samples from the plate and lists them in the Setup > Assays or Setup > Samples workspaces. The assays and samples are stored in these workspaces as a library, and remain there even if you delete the experiment from the Study. Deleting the experiment will remove any data (wells) associated with the assays or samples, but not the assays or samples from the library. The assays and samples must then be deleted from these workspaces to remove them from the Study.