Can Ct's greater than a cut-off be considered valid results?
Yes they can. However, it is important to recognize the true linearity and detection limits of your assay: Ct values above the cut-off can indicate non-specific amplification, unless your NTC is a true- no-target control, and you have run a statistically significant number of replicates. Any results with Ct above the recommended cut-off need to be validated with individual assays on plates.
What is a good Ct cut-off for the TaqMan MicroRNA Array Cards and TaqMan Advanced miRNA Array Cards? In other words, beyond what Ct should I not trust the data?
The typical Ct cut-off on TaqMan Array Cards is 32, which is equivalent to Ct 35 on a plate (10 µl reaction). Previous studies show that if you use pre-amplification, a Ct cut-off of 29 or 30 can be used to reduce numbers of false positives (see Technical Note Optimized protocols for human or rodent microRNA profiling with precious samples). To ensure that you have selected a correct cut-off, you should run replicates of the same sample and use Ct cut-off before you see an increase in the Standard Deviation.
We are upgrading to the Windows 10 operating system. What are the compatible capillary electrophoresis secondary analysis software with Windows 10?
The following capillary electrophoresis secondary analysis software are compatible with Windows 10 (and also Windows 7):
-Sequencing Analysis Software v7
-SeqScape Software v4
-Variant Reporter Software v3
-GeneMapper Software v6
-Minor Variant Finder v1.2
-MicrobeBridge v1.1
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.
Where do I get the latest Applied Biosystems Capillary Electrophoresis Software patches?
Please visit our Applied Biosystems Genetic Analyzer Freeware and Patches website: https://www.thermofisher.com/sangerpatches
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.
How can I import my data from Variant Reporter Software?
For importing data, there are many ways to do so:
More information on backing up the Data Store can be found in the Variant Reporter Help Contents under the keyword “Back-up”.
1. Select the Data Store where you would like the data imported to. To choose a Data Store:
a. Click the Manage button in the Data Store section of the Dashboard View.
b. Highlight the Data Store you wish to use and click on Change Location to select it. Click Close to close the window.
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.
