足够用于:纯化 10 x 0.5 mL IgG 样品• Melon 凝胶 IgG 纯化支持物,1.2 mL • Melon 凝胶纯化缓冲液,100 mL • Zeba 离心脱盐柱,7K MWCO,2 mL,10 根柱 • 离心柱,0.8 mL,10 根柱 • 微量离心管,2 mL,30 个管
储存在 4°C 下
常见问题解答 (FAQ)
Will glycerol pose a problem when purifying antibodies with the Pierce Antibody Clean-up Kit (Cat. No. 44600)?
When using the Pierce Antibody Clean-up Kit, glycerol may pose a problem for the spin desalting columns for buffer exchange, but not for the Melon Gel itself. If glycerol concentrations are high, the solution becomes viscous enough to exert a frictional drag during spin column chromatography and the performance and yield may suffer.
Please find a technical tip on using Melon Gel for cleaning up BSA or gelatin from commercial antibodies.
If the glycerol concentration is higher than 10%, dialysis may be considered to exchange the sample into the Melon Gel buffer. We have some dialysis devices suitable for small volume dialysis, e.g., the Slide-A-Lyzer MINI devices: see here. Alternatively, some of the smaller Slide-A-Lyzer dialysis cassettes can be used: see here.
I want to use the Antibody Clean-Up Kit (Cat. No. 44600) with a purified antibody that has a "protein based stabilizer". Do I need to first perform the Zeba desalting step A in the kit, or can I proceed directly to the BSA/Gelatin Removal step B?
The Zeba desalting step A is necessary as it results in a buffer exchange into the Melon Gel Purification Buffer. Having the antibody in this buffer is critical for the functionality of the Melon Gel used to remove protein.
I typically use BSA or gelatin to store my purified antibody solution for long-term storage. I'm worried that it will interfere with my immunodetection experiment. Is there a way to remove BSA or gelatin from my antibody solution?
The Pierce Antibody Clean-up Kit (Cat. No. 44600) was specifically designed for removal of amine containing molecules from IgG. Please view this Tech Tip (https://tools.thermofisher.com/content/sfs/brochures/TR0055-Melon-Gel-for-BSA.pdf) for additional strategies on how to remove BSA and gelatin from antibody solutions.