Pierce™ 抗体纯化试剂盒
Pierce™ 抗体纯化试剂盒
Thermo Scientific™

Pierce™ 抗体纯化试剂盒

Thermo Scientific Pierce 抗体纯化试剂盒用于在基于胺的化学标记和偶联程序前去除抗体储备液中的 BSA、明胶和其他稳定蛋白。抗体纯化试剂盒的特点:•快速—去除抗体溶液中的载体蛋白所需的时间是通过蛋白 A 或 G了解更多信息
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货号数量
4460010 次反应
货号 44600
价格(CNY)
3,632.00
Each
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数量:
10 次反应
请求批量或定制报价
价格(CNY)
3,632.00
Each
添加至购物车
Thermo Scientific Pierce 抗体纯化试剂盒用于在基于胺的化学标记和偶联程序前去除抗体储备液中的 BSA、明胶和其他稳定蛋白。

抗体纯化试剂盒的特点:

快速—去除抗体溶液中的载体蛋白所需的时间是通过蛋白 A 或 G 纯化程序纯化抗体所需时间的一半
温和 无需通过中和或脱盐降低苛刻洗脱缓冲液的影响
完整—提供脱盐柱,使缓冲液易于交换至所需的纯化缓冲液中或快速交换至所选最终缓冲液中
经济实惠—试剂足以进行 10 次抗体纯化反应

这是一种完整且易于使用的试剂盒,用于去除 IgG 样品中的 BSA 和明胶稳定蛋白(高达 1%)。该方案使用 Thermo Scientific Melon 凝胶结合这些常用的贮藏蛋白,使得抗体能够以纯化的形式流通。该抗体回收到无胺和无羧基的缓冲液中,可随时用于下游应用,如生物素化、荧光标记、交联、固定和酶促片段化(例如 Fab 制备)。

通常将牛血清白蛋白 (BSA) 和明胶添加到纯化的抗体溶液中作为长期储存的稳定剂。包含 0.2 至 1%(2 至 10 mg/mL)BSA 或明胶,有助于稳定稀释的抗体溶液。通常,这些加入的蛋白不会干扰免疫检测方法;然而,它们存在于溶液中,会干扰胺反应性偶联化学,后者是大多数生物素化、荧光染料标记、抗体片段化和共价固定方法的基础。对于这种方法,必须从稳定蛋白中分离出抗体,而抗体纯化试剂盒可以高效地完成这一工作。

相关产品
Melon™ 凝胶 IgG 离心纯化试剂盒
仅供科研使用。不可用于诊断程序。
规格
纯化目标IgG 抗体
数量10 次反应
色谱柱类型具有专利技术的树脂、亲和性
产品规格试剂盒、微量离心机、离心柱
产品线Pierce™
产品类型抗体纯化试剂盒
固定相具有专利技术的配体
Unit SizeEach
内容与储存
足够用于:纯化 10 x 0.5 mL IgG 样品• Melon 凝胶 IgG 纯化支持物,1.2 mL
• Melon 凝胶纯化缓冲液,100 mL
• Zeba 离心脱盐柱,7K MWCO,2 mL,10 根柱
• 离心柱,0.8 mL,10 根柱
• 微量离心管,2 mL,30 个管

储存在 4°C 下

常见问题解答 (FAQ)

Will glycerol pose a problem when purifying antibodies with the Pierce Antibody Clean-up Kit (Cat. No. 44600)?

When using the Pierce Antibody Clean-up Kit, glycerol may pose a problem for the spin desalting columns for buffer exchange, but not for the Melon Gel itself. If glycerol concentrations are high, the solution becomes viscous enough to exert a frictional drag during spin column chromatography and the performance and yield may suffer. Please find a technical tip on using Melon Gel for cleaning up BSA or gelatin from commercial antibodies.

If the glycerol concentration is higher than 10%, dialysis may be considered to exchange the sample into the Melon Gel buffer. We have some dialysis devices suitable for small volume dialysis, e.g., the Slide-A-Lyzer MINI devices: see here. Alternatively, some of the smaller Slide-A-Lyzer dialysis cassettes can be used: see here.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

I want to use the Antibody Clean-Up Kit (Cat. No. 44600) with a purified antibody that has a "protein based stabilizer". Do I need to first perform the Zeba desalting step A in the kit, or can I proceed directly to the BSA/Gelatin Removal step B?

The Zeba desalting step A is necessary as it results in a buffer exchange into the Melon Gel Purification Buffer. Having the antibody in this buffer is critical for the functionality of the Melon Gel used to remove protein.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

I typically use BSA or gelatin to store my purified antibody solution for long-term storage. I'm worried that it will interfere with my immunodetection experiment. Is there a way to remove BSA or gelatin from my antibody solution?

The Pierce Antibody Clean-up Kit (Cat. No. 44600) was specifically designed for removal of amine containing molecules from IgG. Please view this Tech Tip (https://tools.thermofisher.com/content/sfs/brochures/TR0055-Melon-Gel-for-BSA.pdf) for additional strategies on how to remove BSA and gelatin from antibody solutions.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.