Does the Slide-A-Lyzer Dialysis Cassette leak?
The innovative, patented design does not permit “wicking” that can occur with homemade microdialysis devices.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
What sizes are available for the Slide-A-Lyzer Dialysis Cassette? What MWCO membranes are available?
Four sizes to accommodate samples between 0.1 and 30 ml are currently available in five different MWCOs (2K, 3.5K, 7K, 10K and 20K).
The G2 Slide A lyzers on the other hand are available in five different sizes to accommodate samples between 0.25 and 70mL.
These units, especially those that accommodate smaller volumes, provide for greater sample recovery and shorter dialysis times than one can achieve with traditional tubing. We have a cassette selection guide to help you choose the appropriate cassette for your application. There are also product demonstrations for this and other High-Performance Dialysis Products.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
What prevents the Slide-A-Lyzer Dialysis Cassette from leaking?
An internal gasket, which isn't visible after the cassette is assembled, reseals itself as the needle is withdrawn. To maintain sample volume and integrity, users should remember these three key rules:
1. Before use, fill the cassette with air to verify that the system is airtight.
2. Puncture each porthole only once.
3. Do not allow the membrane to come into contact with the sharp needle bevel.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
What is the purpose of the buoy for the Slide-A-Lyzer Dialysis Cassette?
The buoy suspends the Slide-A-Lyzer Cassette above the stirring bar during dialysis and it provides a benchtop stand on which to set the Slide-A-Lyzer Cassette. These are not required with the G2 Slide-A-Lyzer Dialysis Cassettes.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
What is the highest temperature and the pH range that is compatible with the Slide-A-Lyzer Cassette?
Slide-A-Lyzer Cassettes can be held at 56 degrees C for two hours. At temperatures and times above these, the plastic gets soft and can deform. Thermo Scientific recommends that users maintain a pH range of 5-9.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
