PrimeView Human Gene Expression Array
PrimeView Human Gene Expression Array
Applied Biosystems™

PrimeView Human Gene Expression Array

PrimeView Human Gene Expression Array 使您能够:• 每份样品测量超过 36,000 个转录本和变体的基因表达• 通过对每个转录本进行多次独立测量,获得准确且可重现的基因表达数据了解更多信息
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货号阵列数量
90183830 阵列
90183710 阵列
货号 901838
价格(CNY)
-
阵列数量:
30 阵列
PrimeView Human Gene Expression Array 使您能够:
• 每份样品测量超过 36,000 个转录本和变体的基因表达
• 通过对每个转录本进行多次独立测量,获得准确且可重现的基因表达数据

符合行业标准的性能证明
GeneChip PrimeView Human Gene Expression Array 盒采用探针集实现表达谱分析,并强调已经证实、注释充分的内容。阵列设计中使用的序列选自 RefSeq 版本 36、UniGene 数据库 219 和 GenBank™ 中的全长人类 mRNA。

关键优势
• 提供出色的基因表达特异性和重现性
• 每个转录本可通过多项独立测量获得可靠的结果 — 对于注释充分的序列,每组 11 个探针,对于其他的,每组 9 个探针
• 提供完全覆盖的基因组注释
• 业界较高的转录本覆盖率,并带有较新注释 — 覆盖来自 RefSeq 的所有带注释基因和转录本

内容概况
•PrimeView Human Gene Expression Array 由 530,000 多个探针集成,涵盖 36,000 多个转录本和变体,代表通过 RefSeq 或 UniGene 注释定位的 20,000 多个基因。
•将设计中使用的 EST 和 mRNA 序列进行聚类和组装,以创建代表选择性剪接的共有序列。然后对每个组件进行分析,以确定方向和替代的 3'端证据。
• 选择内容涵盖 RefSeq v36 的所有注释良好的基因和转录本,并利用所有落入同一簇的 EST 和 mRNA 证据来严格检测这些具有良好注释的基因的 3'末端。
•超过1,000个探针组代表在 UniGene 中没有正式基因符号的转录本,但基于预测的 RefSeq 序列和 UniGene 簇,并具有实际转录注释基因的良好证据。
仅供科研使用。不可用于诊断程序。
规格
数量30 arrays
类型人基因表达阵列
数组转录组谱分析
产品规格阵列检测盒
阵列数量30 阵列
种属
Unit SizeEach

常见问题解答 (FAQ)

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How are transcript IDs assigned for WT arrays if a gene has multiple transcript IDs?

The first assigned mRNA/gene that is listed in the annotation file for a given probe set or transcript cluster is considered the "best" assignment based on data source quality ranking and assignment scoring system. To get a single best mRNA/gene assignment you can simply take the first one in the list in the annotation file.

Sometimes, there might be multiple best hits between transcript clusters (or probe set) and a mRNA/gene, with identical score and data source quality. In that case, the transcript assignments are ranked based on their descriptions (e.g., title from the GenBank record).

For transcript clusters that detect genes that are alternatively spliced, the different spliced forms will be ranked by length with the longest ones listed first.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is an example of using ERCC controls in the PrimeView Human Gene Expression Array?

The following link is an example of how the ERCC control probes on the Primeview Human Gene Expression Array can be used: https://pubmed.ncbi.nlm.nih.gov/23101621/.
Please note that the ERCC controls, while tiled on the array itself, are not visible in the commercial library files. A CDF file does exist with these made visible, however. Please contact Technical Support to access a copy of this.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Does the GeneChip miRNA 3.1 Array Strip require a different hyb/wash/stain kit than the GeneChip 3' IVT arrays and GeneChip ST array strips?

The hyb/wash/stain kit for the GeneChip miRNA 3.1 Array Strips is different than that for the 3' IVT arrays and GeneChip ST array strips. Additional wash A steps have been added to ensure adequate washing after the array strips have been stained.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Are the PrimeView Human Genome U219 Array Strip and PrimeView Human Genome U219 Array Plate the same as the PrimeView Human Gene Expression Array?

Yes, the PrimeView Human U219 Array and the Primeview Human Gene Expression Array are indeed the same array (including strip and plate).

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.