Expi293™ 表达培养基
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Expi293™ 表达培养基
Gibco™

Expi293™ 表达培养基

Gibco Expi293 表达培养基是一种化学成分确定、无血清、无蛋白的培养基,用于培养和转染悬浮的人胚肾 (HEK) 293 细胞。此培养基是 Expi293了解更多信息
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货号数量
A143510310 L
A143510420 L
A14351011 L
A14351026 x 1 L
货号 A1435103
价格(CNY)
38,090.00
Each
添加至购物车
数量:
10 L
价格(CNY)
38,090.00
Each
添加至购物车
Gibco Expi293 表达培养基是一种化学成分确定、无血清、无蛋白的培养基,用于培养和转染悬浮的人胚肾 (HEK) 293 细胞。此培养基是 Expi293 表达系统的核心组成部分,专门用于在系统中进行高密度的 Expi293F 细胞培养,以实现可扩展的瞬时蛋白表达。

•支持悬浮 293 培养物生长直至密度超过 1.5 x 10^7 个细胞/mL
• 该无血清悬浮培养基兼容转染,与 ExpiFectamine 转染试剂配合使用时,转染效率超过 70%
• 能够持续、高水平、高密度地进行瞬时蛋白表达,重组蛋白产量高达 1 g/L
•支持 293 细胞在各种培养规模下(从多孔板中的不到 1mL 到一次性生物反应器中的大于 10L)的生长和转染
• 不含酚红

Expi293 表达培养基含有 GlutaMAX-I 。可直接使用,无需添加剂。化学成分确定的配方可实现高度的重复性和批间可靠性。Expi293 表达培养基不含人源性或动物源性成分。不推荐将此培养基用于贴壁细胞培养。

产品用途
在生产工艺中使用 Expi293 表达培养基并已向 FDA 提交审核申请的客户,可向本公司索取授权函,以便引用本公司的 II 类药品主文件 (DMF)。

仅用于研究和生产用途。不可用于诊断。
规格
细胞系Expi293F™
产品线Expi293
产品类型表达培养基
数量10 L
分类非动物源性, 化学成分明确, 无蛋白, 无血清
形式液体
加有添加剂GlutaMAX
不加添加剂无酚红
Unit SizeEach
内容与储存
一个 10L 通用袋
储存于 2-8°C 的阴暗处。

常见问题解答 (FAQ)

我能使用ProBond或Ni-NTA纯化系统来纯化Expi293表达培养基中的目的蛋白吗?

Expi293表达培养基并不直接兼容ProBond或Ni-NTA纯化系统。我们推荐在用His标签的纯化操作之前进行一次缓冲体系交换或样本透析。

Can I purify my protein from Expi293 Expression Medium using ProBond or Ni-NTA purification systems?

Expi293 Expression Medium is not directly compatible with ProBond or Ni-NTA purification systems. We recommend performing a buffer exchange or dialyzing the samples before His-tag purification.

Can I shake Expi293 cultures faster or slower than recommended?

For most flasks, there is a dropoff in expression when you go too fast or too slow because of cell shear stress or insufficient aeration. The effect is greater in plates where there is a sharper transition between static and moving fluid in the wells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My Expi293 Expression Medium (Cat. No. A1435102) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does Expi293 Expression Medium contain antibiotics?

Expi293 Expression Medium does not contain antibiotics. We do not recommend the use of antibiotics, however, 5 mL/L of Antibiotic-Antimycotic (Cat. No. 15240) containing penicillin, streptomycin, and amphotericin B may be used when required, as stated on page 1 in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/expi293_exp_medium_man.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.