Can I purify my protein from Expi293 Expression Medium using ProBond or Ni-NTA purification systems?
Expi293 Expression Medium is not directly compatible with ProBond or Ni-NTA purification systems. We recommend performing a buffer exchange or dialyzing the samples before His-tag purification.
Can I shake Expi293 cultures faster or slower than recommended?
For most flasks, there is a dropoff in expression when you go too fast or too slow because of cell shear stress or insufficient aeration. The effect is greater in plates where there is a sharper transition between static and moving fluid in the wells.
My Expi293 Expression Medium (Cat. No. A1435102) was accidentally frozen. Can I still use it?
This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.
Does Expi293 Expression Medium contain antibiotics?
Expi293 Expression Medium does not contain antibiotics. We do not recommend the use of antibiotics, however, 5 mL/L of Antibiotic-Antimycotic (Cat. No. 15240) containing penicillin, streptomycin, and amphotericin B may be used when required, as stated on page 1 in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/expi293_exp_medium_man.pdf).