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引用和文献 (15)
Invitrogen™
Phalloidin Labeling Probes
Achieve precise and reliable F-actin staining with fluorescent and biotinylated phalloidins. Phalloidin conjugates are widely used in imaging applications to selectively label F-actin in a variety of sample types including fixed and permeabilized cells, tissue sections, and cell-free experiments.
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| 货号 | 颜色 | 激发波长范围 | 染料类型 |
|---|---|---|---|
| A22285 | Near-infrared | 663⁄690 | Alexa Fluor™ 660 |
| A22281 | Blue | 346⁄442 | Alexa Fluor™ 350 |
| A30104 | Violet | 405/450 | Alexa Fluor™ Plus 405 |
| A12379 | Green | 激发波长495 nm 发射波长518 nm | Alexa Fluor™ 488 |
| O7466 | Green | 496⁄520 | Oregon Green™ 488 |
| F432 | Green | 496⁄516 | FITC(荧光素) |
| A22282 | Yellow | 531⁄554 | Alexa Fluor™ 532 |
| R415 | Red-orange | 540⁄565 | TRITC(四甲基异硫氰酸罗达明) |
| A22283 | Orange | 556⁄570 | Alexa Fluor™ 546 |
| A34055 | Orange | 555⁄565 | Alexa Fluor™ 555 |
| A30106 | Orange | 555/565 nm | Alexa Fluor Plus 555 |
| B3475 | Red | 558⁄569 | BODIPY™ |
| A12380 | Orange-red | 578⁄600 | Alexa Fluor™ 568 |
| A12381 | Red | 581⁄609 | Alexa Fluor™ 594 |
| T7471 | Red | 591⁄608 | Texas Red™ |
| A22284 | Far-red | 632⁄647 | Alexa Fluor™ 633 |
| A34054 | Far-red | 633⁄647 | Alexa Fluor™ 635 |
| A22287 | Far-red | 650⁄668 | Alexa Fluor™ 647 |
| A30107 | Far-red | 650/668 nm | Alexa Fluor Plus 647 |
| A22286 | Near-infrared | 679⁄702 | Alexa Fluor™ 680 |
| A30105 | Near-infrared | 758/784 | Alexa Fluor™ Plus 750 |
货号 A22285
价格（CNY)
-
颜色:
Near-infrared
激发波长范围:
663⁄690
染料类型:
Alexa Fluor™ 660
Fluorescent and biotinylated phalloidins are water soluble and bind to filamentous actin (F-actin) with nanomolar affinity, making them convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, fixed and permeabilized cells, and cell-free experiments. Phalloidin conjugates bind similarly to actin from various species, including plants and animals, enabling staining of the cytoskeleton in a wide range of samples.
A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.
Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.
Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining
Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.
Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.
Features of phalloidin probes
- High specificity—binds selectively to F-actin, which allows for precise labeling of actin filaments in fixed cells and cryopreserved tissues
- Strong affinity—nanomolar binding affinity for F-actin, which ensures stable and reliable actin staining
- Extensive fluorescent conjugate options—over twenty conjugated varieties of phalloidin
- Compatibility with fixed samples—typically used with fixed cells and tissues, making them suitable for actin staining in detailed structural studies, immunofluorescence staining, and IHC applications
- Multiplexing capability—the wide availability of phalloidin conjugates enables their use in combination with other fluorescent probes and antibodies for multiplex imaging. Biotinylated phalloidin can be made use of in downstream streptavidin steps.
- Quantitative analysis—can be used for quantitative analysis of F-actin distribution and density within cells, aiding in the study of cytoskeletal dynamics. The unlabeled phalloidin can be titrated as a control.
- Ease of use—staining is straightforward and quick
- Excellent stability—exhibit good photostability, which is essential for prolonged imaging sessions and time-lapse studies
- Wide applicability—used for a range of applications, including studying cell morphology, motility, and the effects of drugs on the actin cytoskeleton
仅供科研使用。不可用于诊断程序。
规格
颜色Near-infrared
染料类型Alexa Fluor™ 660
激发波长范围663⁄690
适用于（设备）Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Compatible Cy5/Cy5.5 filter set
产品线Alexa Fluor
数量300 Units
运输条件室温
标签类型Alexa Fluor 染料
产品类型鬼笔环肽
亚细胞定位肌动蛋白、细胞骨架, Cytoskeleton
Unit SizeEach
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。
常见问题解答 (FAQ)
我试着用鬼笔环肽结合F-actin标记我的石蜡切片，但是没有看到信号，为什么？
Can I combine Click-iT or Click-iT Plus reactions with phalloidin conjugates used for actin staining?
I'm trying to label my paraffin sections for F-actin with a phalloidin conjugate, but I'm not seeing any signal. Why?
引用和文献 (15)
引用和文献
Abstract
Myosin Va maneuvers through actin intersections and diffuses along microtubules.
Journal:Proc Natl Acad Sci U S A
PubMed ID:17360524
'Certain types of intracellular organelle transport to the cell periphery are thought to involve long-range movement on microtubules by kinesin with subsequent handoff to vertebrate myosin Va (myoVa) for local delivery on actin tracks. This process may involve direct interactions between these two processive motors. Here we demonstrate using single
Sidekicks: synaptic adhesion molecules that promote lamina-specific connectivity in the retina.
Journal:Cell
PubMed ID:12230981
A major determinant of specific connectivity in the central nervous system is that synapses made by distinct afferent populations are restricted to particular laminae in their target area. We identify Sidekick (Sdk)-1 and -2, homologous transmembrane immunoglobulin superfamily molecules that mediate homophilic adhesion in vitro and direct laminar targeting of
Two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis.
Journal:Plant Cell Physiol
PubMed ID:15047875
We show that two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis, that have been chemically fixed with a combination of methylglyoxal and formaldehyde and stained with Alexa phalloidin. In approximately one half of the hyphae examined, an F-actin depleted zone within the
A role for PKC-epsilon in Fc gammaR-mediated phagocytosis by RAW 264.7 cells.
Journal:J Cell Biol
PubMed ID:12499353
Protein kinase C (PKC) plays a prominent role in immune signaling, and the paradigms for isoform selective signaling are beginning to be elucidated. Real-time microscopy was combined with molecular and biochemical approaches to demonstrate a role for PKC- epsilon in Fc gamma receptor (Fc gammaR)-dependent phagocytosis. RAW 264.7 macrophages were
The HIV-1 pathogenicity factor Nef interferes with maturation of stimulatory T-lymphocyte contacts by modulation of N-Wasp activity.
Journal:J Biol Chem
PubMed ID:16687395
The Nef protein is a key determinant of human immunodeficiency virus (HIV) pathogenicity that, among other activities, sensitizes T-lymphocytes for optimal virus production. The initial events by which Nef modulates the T-cell receptor (TCR) cascade are poorly understood. TCR engagement triggers actin rearrangements that control receptor clustering for signal initiation