Expi293™ Met (–) 表达培养基

Expi293 Met (–) 表达培养基是按照 cGMP 生产的、化学成分明确、无血清、无蛋白、非动物源性了解更多信息

货号	数量
A4096701	1 L

货号 A4096701

价格（CNY)

7,352.00

添加至购物车

价格（CNY)

7,352.00

添加至购物车

Expi293 Met (–) 表达培养基是按照 cGMP 生产的、化学成分明确、无血清、无蛋白、非动物源性、无蛋氨酸培养基，用于 Expi293F 细胞的生长和转染。培养基是 Expi293 平台的一部分，用于哺乳动物 HEK293 细胞的快速、高产量瞬时蛋白表达。其设计用于与 ExpiFectamine 293 Met (–) 转染试剂盒结合使用，用于需要标记蛋白中蛋氨酸残基的应用。注：除非补充了蛋氨酸，否则培养基不支持细胞生长或表达。

Expi293 Met (–) 表达培养基特点包括：
•支持悬浮 293 培养物的生长，使密度达到超过 1.5 x 10⁷ 个细胞/mL
•与 Expi293 表达培养基（货号A1435101）的性能相当
• 不含蛋氨酸
• 不含酚红
• 使用 GlutaMAX I 补充剂配制
• 需要补充蛋氨酸

L-蛋氨酸（甲基-¹³C）和 L-硒代蛋氨酸标记试剂单独提供。

仅供科研使用。不可用于诊断程序。

细胞系Expi293F™

分类AOF、化学成分明确、无蛋氨酸、无蛋白、无血清

产品线Expi293

产品类型293 表达培养基

数量1 L

运输条件室温

Unit SizeEach

内容与储存

1 L Expi293 Met (–) 表达培养基，在 2–8°C 下储存（避光储存）

常见问题解答 (FAQ)

I am performing L-selenomethionine labeling and getting very low protein yields. What could be going wrong?

L-selenomethionine is a known cell toxin. Therefore, it is important to grow cells in the present of this amino acid for the shortest amount of time required to achieve maximal labeling. We recommend adding L-selenomethionine to the culture just prior to transfection (in Expi293 Met (-) Expression Medium). You may also starve cells for 6 hr by culturing cells in Expi293 Met (-) Expression Medium (without supplementation) and then proceed with L-selenomethionine add-back and transfection.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I'm culturing my Expi293F cells in Expi293 Met (-) Expression Medium but they are not growing. What could be going wrong?

Expi293 Met (-) Expression Medium does not contain methionine, which is an essential nutrient for cell culture. Therefore, methionine supplementation is required. For methionine labeling, cells should be grown in Expi293 Met (-) Expression Medium and starved (i.e., grown in the absence of methionine supplementation) for 6 hr prior to addition of 13C-methyl-L-methionine, or similar methionine isotope.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How do I use Expi293 Met (-) Expression Medium for methionine protein labeling?

Expi293 Met (-) Expression Medium is not intended to support long-term cell culture as the media is deficient of methionine, an essential amino acid for cell survival. When you are ready to start your labeling experiment, transfer Expi293F Cells growing in Expi293 Expression Medium to Expi293 Met (-) Expression Medium by centrifuging the cell suspension (200 x g for 5 min) and resuspending the cell pellet in Expi293 Met (-) Expression Medium. Cells may be starved for 6 hr prior to supplementation with L-selenomethionine, isotopic-labeled methionine, or other derivatives.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the difference between Expi293 Expression Medium and Expi293 Met (-) Expression Medium?

The formulation of Expi293 Met (-) Expression Medium is identical to that of Expi293 Expression Medium except for the absence of methionine. Expi293 Met (-) Expression Medium allows for the addition of isotopic-labeled methionine or derivatives to promote labeling of proteins for structural analysis.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.