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引用和文献 (19)
Invitrogen™
Click-iT™ AHA Alexa Fluor™ 488 蛋白质合成 HCS 检测
Click-iT® AHA Alexa Fluor® 488 蛋白质合成 HCS 检测试剂盒为检测新生蛋白质合成提供了一种快速、灵敏、无毒且无放射性的方法，可利用荧光显微镜和高内涵成像进行检测了解更多信息
| 货号 | 数量 |
|---|---|
| C10289 | 2 plates |
货号 C10289
价格（CNY)
6,839.00
飞享价
Ends: 31-Dec-2025
9,271.00共减 2,432.00 (26%)
Each
数量:
2 plates
价格（CNY)
6,839.00
飞享价
Ends: 31-Dec-2025
9,271.00共减 2,432.00 (26%)
Each
Click-iT® AHA Alexa Fluor® 488 蛋白质合成 HCS 检测试剂盒为检测新生蛋白质合成提供了一种快速、灵敏、无毒且无放射性的方法，可利用荧光显微镜和高内涵成像进行检测。Click-iT® AHA 是一种含有叠氮基团的蛋氨酸类似物。与 35S-蛋氨酸类似，Click-iT® AHA 可以添加至培养的细胞并在活性蛋白质合成过程中掺入蛋白质内。对掺入的胺酸进行的检测利用了叠氮化物和炔烃之间的化学选择性连接或 “click" 反应，使用绿色荧光 Alexa Fluor® 488 炔烃检测经过叠氮基修饰的蛋白质。Click-iT® AHA Alexa Fluor® 488 蛋白质合成 HCS 检测试剂盒已经在 549 和 U-2 OS 细胞中使用多种抑制蛋白质合成的试剂（包括环己酰亚胺、茴香霉素和嘌呤霉素）以剂量反应性和最小⁄最大剂量的方式成功进行过测试。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型Alexa Fluor™ 488，Hoechst 33258
产品规格96 孔板
环保功能危害更小
数量2 plates
运输条件室温
颜色蓝色、绿色
适用于（应用）蛋白合成 HCS 测定试剂盒
适用于（设备）荧光显微镜, 高内涵分析仪
产品线Alexa Fluor、Click-iT、Molecular Probes
产品类型HCS 检测
Unit SizeEach
内容与储存
试剂盒包含足够测定96孔板规格的2块微孔板的试剂。在 ≤-20°C 下干燥、避光储存。
常见问题解答 (FAQ)
I am using Click-iT AHA (L-azidohomoalanine) kit to label nascent proteins in live cells, then detecting with TAMRA alkyne after fixation and permeabilization and a click reaction. But I'm seeing nucleolar labeling in the cells. It this expected?
引用和文献 (19)
引用和文献
Abstract
Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT).
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:16769897
In both normal and pathological states, cells respond rapidly to environmental cues by synthesizing new proteins. The selective identification of a newly synthesized proteome has been hindered by the basic fact that all proteins, new and old, share the same pool of amino acids and thus are chemically indistinguishable. We
In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons.
Journal:Nature neuroscience
PubMed ID:20543841
Protein translation has been implicated in different forms of synaptic plasticity, but direct in situ visualization of new proteins is limited to one or two proteins at a time. Here we describe a metabolic labeling approach based on incorporation of noncanonical amino acids into proteins followed by chemoselective fluorescence tagging
Two-color labeling of temporally defined protein populations in mammalian cells.
Journal:Bioorganic & medicinal chemistry letters
PubMed ID:18774715
The proteome undergoes complex changes in response to disease, drug treatment, and normal cellular signaling processes. Characterization of such changes requires methods for time-resolved protein identification and imaging. Here, we describe the application of two reactive methionine (Met) analogues, azidohomoalanine (Aha) and homopropargylglycine (Hpg), to label two protein populations in
Fluorescence visualization of newly synthesized proteins in mammalian cells.
Journal:Angewandte Chemie (International ed. in English)
PubMed ID:17036290
Noncanonical amino acid tagging enables the selective fluorescent visualization of newly synthesized proteins in mammalian cells (see the picture). Susceptibility to tagging is determined by the spatial and temporal character of the protein synthesis, thus providing a complement to methods which identify relevant members of the proteome.
Spatial coupling of mTOR and autophagy augments secretory phenotypes.
Journal:Science (New York, N.Y.)
PubMed ID:21512002
Protein synthesis and autophagic degradation are regulated in an opposite manner by mammalian target of rapamycin (mTOR), whereas under certain conditions it would be beneficial if they occurred in unison to handle rapid protein turnover. We observed a distinct cellular compartment at the trans side of the Golgi apparatus, the