Calcium Green™-1，AM，细胞通透性

引用和文献 (99)

Invitrogen™

Calcium Green™-1，AM，细胞通透性

Name: Calcium Green™-1，AM，细胞通透性
SKU: C3012
Price: 6435.00 CNY

标记钙指示剂是结合 Ca2+ 离子后荧光强度增加的分子。其可用于多种钙信号转导研究，包括测量细胞内 Ca2+、跟踪 Ca2+ 进入和释放以及对活组织中 Ca2+ 进行多光子激发成像了解更多信息

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货号	数量
C3012 又称 C-3012	10 x 50μg
C3011MP	500 μg

2 选项

货号 C3012

又称 C-3012

价格（CNY)

6,435.00

Each

添加至购物车

数量:

10 x 50μg

价格（CNY)

6,435.00

Each

添加至购物车

标记钙指示剂是结合 Ca²⁺ 离子后荧光强度增加的分子。其可用于多种钙信号转导研究，包括测量细胞内 Ca²⁺、跟踪 Ca²⁺ 进入和释放以及对活组织中 Ca²⁺ 进行多光子激发成像。将溶解后的指示剂直接加入含有培养细胞的培养皿中，即可向细胞上样 AM 酯形式的这类钙离子指示剂。这类细胞发出的荧光信号通常采用荧光显微镜、荧光微孔板检测法或流式细胞仪测量。

钙指示剂（AM 酯）规格：
• 标记（激发/发射波长）：Calcium Green™ -1 (506/531 nm)
• 结合 Ca²⁺ 后荧光强度增加：∼14 倍
• 结合 Ca²⁺ 之后荧光增加，波长稍有变化

Molecular Probes™ 钙指示剂的光谱特性
这类探针被可见光激发，且由于激发所需的能量较低，引起细胞光损伤的可能性较低。常用的激光仪器（如共聚焦激光扫描显微镜）能够有效激发这类指示剂，且其发射光谱位于通常不受细胞自发荧光和散射背景干扰的光谱区域。

更丰富的钙荧光指示剂的选择
我们提供了大量 Molecular Probes™ 钙指示剂，可用于各种实验方案，例如右旋糖酐形式可减少泄漏和区室化、 BAPTA 偶联物用于检测高幅钙瞬变。更多信息请参阅 Molecular Probes™ 手册可见光激发的 Ca²⁺ 荧光指示剂—第 19.3 节。

对于 UV 激发的 Ca²⁺ 指示剂、基于蛋白的 Ca²⁺ 指示剂、Ca²⁺ 指示剂的偶联物以及其他金属离子（即 Mg²⁺、Zn²⁺）的荧光指示剂，请查看 Molecular Probes™ 手册中的 Ca²⁺、Mg²⁺、Zn²⁺ 以及其他金属离子指示剂—第 19 章。

仅供科研使用。不适用于动物或人类的治疗或诊断。

仅供科研使用。不可用于诊断程序。

规格

检测方法荧光

染料类型基于荧光染料

数量10 x 50μg

运输条件室温

适用于（应用）细胞活力与增殖

适用于（设备）荧光显微镜

产品线Calcium Green

产品类型钙指示剂

Unit SizeEach

内容与储存

在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The K_d value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (99)

引用和文献

Abstract

Mechanism of collagen activation in human platelets.

Authors:Roberts DE, McNicol A, Bose R

Journal:J Biol Chem

PubMed ID:14981087

The mechanism of collagen-induced human platelet activation was examined using Ca2+, Na+, and the pH-sensitive fluorescent dyes calcium green/fura red, sodium-binding benzofuran isophthalate, and 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. Administration of a moderate dose of collagen (10 microg/ml) to human platelets resulted in an increase in [Ca2+](i) and platelet aggregation. The majority of this ... More

The growth-related gene product beta induces sphingomyelin hydrolysis and activation of c-Jun N-terminal kinase in rat cerebellar granule neurones.

Authors:Limatola C, Mileo AM, Giovannelli A, Vacca F, Ciotti MT, Mercanti D, Santoni A, Eusebi F

Journal:J Biol Chem

PubMed ID:10593952

'The growth-related gene product beta (GRObeta) is a small chemoattractant cytokine that belongs to the CXC chemokine family, and GRObeta receptors are expressed in the brain, including the cerebellum. We demonstrate that rat cerebellar granule neurones express the GRObeta receptor CXCR2. We also show that, in addition to the known ... More

Intracellular astrocyte calcium waves in situ increase the frequency of spontaneous AMPA receptor currents in CA1 pyramidal neurons.

Authors:Fiacco TA, McCarthy KD

Journal:J Neurosci

PubMed ID:14736858

'Spontaneous neurotransmitter release and activation of group I metabotropic glutamate receptors (mGluRs) each play a role in the plasticity of neuronal synapses. Astrocytes may contribute to short- and long-term synaptic changes by signaling to neurons via these processes. Spontaneous whole-cell AMPA receptor (AMPAR) currents were recorded in CA1 pyramidal cells ... More

Control of IP(3)-mediated Ca2+ puffs in Xenopus laevis oocytes by the Ca2+-binding protein parvalbumin.

Authors:John LM, Mosquera-Caro M, Camacho P, Lechleiter JD

Journal:J Physiol

PubMed ID:11507154

'1. Elementary events of Ca2+ release (Ca2+ puffs) can be elicited from discrete clusters of inositol 1,4,5 trisphosphate receptors (IP(3)Rs) at low concentrations of IP(3). Ca(2+) puffs have rarely been observed unless elicited by either hormone treatment or introduction of IP(3) into the cell. However, cells appear to have sufficient ... More

Inositol 1,4,5-trisphosphate and ryanodine receptor distributions and patterns of acetylcholine- and caffeine-induced calcium release in cultured mouse hippocampal neurons.

Authors:Seymour-Laurent KJ, Barish ME

Journal:J Neurosci

PubMed ID:7722617

'The distributions of inositol 1,4,5-trisphosphate and ryanodine receptors (InsP3R and RyR) and the patterns of increase in intracellular calcium ion concentration ([Ca2+]i) elicited by their activation were compared in cultured hippocampal neurons. InsP3R and RyR were labeled using specific antibodies and formed small aggregations in the somata and dendrites of ... More

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