含有一小瓶 Lipofectamine CRISPRMAX 试剂和一小瓶 Cas9 Plus 试剂。
在 2–8°C 下储存。切勿冷冻。
常见问题解答 (FAQ)
Can I use Lipofectamine CRISPRMAX Cas9 Transfection Reagent with one-cell embryos?
We have no data regarding the use of CRISPRMAX in one-celled embryos, therefore we do not recommend using this product for this application.
The shipping for my Lipofectamine CRISPRMAX Cas9 Transfection Reagent was delayed. Can I still use it?
All of the Lipofectamine reagents, including Lipofectamine CRISPRMAX Cas9 Transfection Reagent, are stable at room temperature for months. What is most important is that the reagent did not freeze, because that will alter the integrity and composition of the lipid particles and will compromise usability. Previous freezing can be evidenced by precipitation and excessive cloudiness of the reagent. A limited amount of cloudiness is normal, but excessive cloudiness is a sign that the reagent froze at some point. If the reagent you received looks clear or a bit cloudy the product should still be usable.
Some additional data on room temperature stability of some Lipofectamine reagents, in the context of shipping, can be found in this white paper.
How much Lipofectamine CRISPRMAX Reagent, Cas9 protein, and gRNA should I complex for gene editing?
Please see the Lipofectamine CRISPRMAX Reagent manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0014545_lipofectamine_crispermax_QR.pdf) for protocol details or contact techsupport@thermofisher.com.
How does the Lipofectamine CRISPRMAX Reagent work?
The Lipofectamine CRISPRMAX Reagent combined with the proprietary enhancement properties of the Lipofectamine Cas9 Plus Reagent leads to efficient complex formation with the Cas9-gRNA ribonucleoprotein, for best delivery to the nucleus, helping to ensure high gene editing frequency for a wide range of cell types.
Can the Lipofectamine CRISPRMAX Reagent be used to deliver proteins other than the Cas9 nuclease?
Although the Lipofectamine CRISPRMAX Reagent was developed for the delivery of the Cas9-gRNA ribonucleoprotein, there is potential for other protein complex applications.