OxyBURST™ Green H2DCFDA，SE
OxyBURST™ Green H2DCFDA，SE
引用和文献 (19)
Invitrogen™

OxyBURST™ Green H2DCFDA，SE

OxyBURST™ Green H2DCFDA 琥珀酰亚胺酯（2',7'-二氯二氢荧光素二乙酸酯，SE）是一种胺反应性检测试剂，可用于制备多种生物分子和颗粒的氧化敏感偶联物，包括抗体、抗原、肽、蛋白、葡聚糖了解更多信息
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货号数量
D29355 mg
货号 D2935
价格（CNY)
2,367.00
飞享价
Ends: 31-Dec-2025
3,104.00
共减 737.00 (24%)
Each
添加至购物车
数量:
5 mg
价格（CNY)
2,367.00
飞享价
Ends: 31-Dec-2025
3,104.00
共减 737.00 (24%)
Each
添加至购物车
OxyBURST™ Green H2DCFDA 琥珀酰亚胺酯（2',7'-二氯二氢荧光素二乙酸酯，SE）是一种胺反应性检测试剂，可用于制备多种生物分子和颗粒的氧化敏感偶联物，包括抗体、抗原、肽、蛋白、葡聚糖、细菌、酵母和聚苯乙烯微球等。OxyBURST™ Green H2DCFDA 与胺偶联后，在中性 pH 值环境下用羟胺处理除去两个乙酸酯，生成二氢荧光素偶联物。OxyBURST™ Green H2 DCFDA 偶联物不发荧光，但经氧化后会形成相应的荧光素衍生物。

ROS 指示剂质量标准：
• Ex/Em：∼492–495/517–527 nm
• 产品对空气敏感，应储存在干燥的氩气或氮气中
• 产品可溶于 DMSO、DMF 或乙醇中使用
• 指示剂可渗透细胞（细胞上样方案见文献）
• 可使用流式细胞仪、荧光计、微孔板读数仪或荧光显微镜（配有适用于荧光素的激发光源和滤光片）对荧光信号进行监测

查找更多 ROS 指示剂
我们提供了一系列 Molecular Probes™ 产品，用于生成活性氧簇 (ROS)（包括单线态氧、超氧化物、羟基自由基、多种过氧化物和过氧化氢）以及用于在溶液中进行相应荧光检测。有关这些产品的更多信息，请查阅 Molecular Probes™ 手册第 18.2 节—生成和检测活性氧。

供研究使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
数量5 mg
产品类型ROS Indicator
Unit SizeEach

常见问题解答 (FAQ)

I want to assay cells for reactive oxygen species using carboxy-H2DCFDA, but I want to do so with a plate reader instead of microscope. Will it work?

It has been done. The problem is that plate readers are less sensitive than microscopes, with far less signal-to-background difference. It is worth trying, but first optimize concentrations and loading times with control cells, use a plate with little to no autofluorescence, and possibly optimize the gain setting in order to get the best signal possible. But don't expect the same sensitivity, even with optimization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have GFP-transfected cells and need to label for reactive oxygen species. Can I use H2DCFDA?

This is not recommended as the two dyes overlap in the emission wavelength. There are other ROS reagents available in different wavelengths, such as CellROX Deep Red, which emits in the far-red range (665 nm), or dihydroethidium, which is emits in the visible red range (620 nm).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cell with CM-H2DCFDA for reactive oxygen detection, but upon illuminating the cell there is a significant increase in fluorescence in the control cells. Why?

If the cell is overloaded with dye, the high intracellular concentration of the dye may lead to dye-dye quenching. Upon illumination, photobleaching will occur, which will reduce the dye-dye quenching and actually increase the fluorescence (for a while, but then it will start decreasing). To solve the problem, reduce the concentration and incubation time, and try a range of incubation times and concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a formaldehyde-fixable reactive oxygen species detection assay. Is H2 DCFDA fixable?

H2DCFDA and similar derivatives are not fixable. The same goes for dihydroethidium and dihydrorhodamine. However, CellROX Deep Red and CellROX Green are retained for a limited time upon fixation with formaldehyde. CellROX Green may be retained upon subsequent Triton X-100 permeabilization. Avoid the use of any acetone or alcohol-based fixatives or fixatives that include alcohol, such as formalin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (19)

引用和文献
Abstract
Effect of C1q on the processing of immune complexes by human neutrophils.
Authors:Ohkuro M, Kobayashi K, Takahashi K, Nagasawa S
Journal:Immunology
PubMed ID:7835978
'We prepared immune complexes (IC) composed of human anti-tetanus toxoid IgG and tetanus toxoid, and examined the effect of C1q on the processing of IC by human neutrophils. Treating IC with increasing amounts of C1q enhanced the binding and phagocytosis of IC by neutrophils, unless the amounts of C1q added ... More
Initial cytoplasmic and phagosomal consequences of human neutrophil exposure to Staphylococcus epidermidis.
Authors:Bernardo J, Long HJ, Simons ER,
Journal:Cytometry A
PubMed ID:19937952
'Microorganisms are recognized by specific phagocyte surface receptors. Liganded receptors then signal a series of events leading to phagocytosis and destruction of the organism by oxidative, lytic, and associated processes. Some organisms, such as Mycobacterium tuberculosis (Mtb), Cryptococcus neoformans (Cf), and others, evade such destruction, surviving and sometimes multiplying within ... More
Stable accumulation of p67phox at the phagosomal membrane and ROS production within the phagosome.
Authors:Tlili A, Erard M, Faure MC, Baudin X, Piolot T, Dupré-Crochet S, Nüsse O,
Journal:J Leukoc Biol
PubMed ID:21954286
'Production of ROS by the leukocyte NADPH oxidase is essential for the destruction of pathogenic bacteria inside phagosomes. The enzyme is a complex of cytosolic and membranous subunits that need to assemble upon activation. Biochemical data suggest that the complex is renewed continuously during activity. Furthermore, it is generally assumed ... More
Reactive oxygen species-producing site in radiation and hydrogen peroxide-induced apoptosis of human peripheral T cells: Involvement of lysosomal membrane destabilization.
Authors:Ogawa Y, Kobayashi T, Nishioka A, Kariya S, Ohnishi T, Hamasato S, Seguchi H, Yoshida S
Journal:Int J Mol Med
PubMed ID:15067365
'In our previous studies, we showed that the apoptotic resistance of the human osteosarcoma cell line HS-Os-1 against irradiation was easily converted to a state of apoptotic-susceptibility by the addition of a relatively low concentration of hydrogen peroxide to the culture medium just prior to irradiation. When we consider the ... More
Malaria inhibits surface expression of complement receptor 1 in monocytes/macrophages, causing decreased immune complex internalization.
Authors:Fernandez-Arias C, Lopez JP, Hernandez-Perez JN, Bautista-Ojeda MD, Branch O, Rodriguez A,
Journal:J Immunol
PubMed ID:23440418
Complement receptor 1 (CR1) expressed on the surface of phagocytic cells binds complement-bound immune complexes (IC), playing an important role in the clearance of circulating IC. This receptor is critical to prevent accumulation of IC, which can contribute to inflammatory pathology. Accumulation of circulating IC is frequently observed during malaria, ... More
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