二氢罗丹明 123
二氢罗丹明 123
引用和文献 (377)
Invitrogen™

二氢罗丹明 123

二氢罗丹明 123是一种不带电荷且无荧光的活性氧 (ROS) 指示剂，可被动扩散透过细胞膜，在膜上氧化成阳离子罗丹明 123，后者聚集在线粒体中并显示绿色荧光。了解更多信息
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货号数量
D63210 mg
货号 D632
价格（CNY)
5,091.00
Each
添加至购物车
数量:
10 mg
价格（CNY)
5,091.00
Each
添加至购物车
二氢罗丹明 123是一种不带电荷且无荧光的活性氧 (ROS) 指示剂，可被动扩散透过细胞膜，在膜上氧化成阳离子罗丹明 123，后者聚集在线粒体中并显示绿色荧光。
仅供科研使用。不可用于诊断程序。
规格
数量10 mg
运输条件Room Temperature
Unit SizeEach

常见问题解答 (FAQ)

I am using the reactive oxygen species detection dye, Dihydrorhodamine 123, and when I put the dye in a solution of water and illuminate with UV light, I see a quick change in signal, even without cells. What is happening and how can I stop this?

You cannot stop it. Any time you illuminate a dye, including this one, photodegradation of the dye will lead to the production of singlet oxygen and free radicals. This in turn causes many ROS indicators, like this one, to become oxidized. If you are testing ROS using UV illumination, all you can do is use this as a baseline and subtract the signal from the sample with cells. The best you can do is reduce the illumination intensity (though this will also reduce the specific signal emission).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (377)

引用和文献
Abstract
Nuclear factor-kappaB mediates the cell survival-promoting action of activity-dependent neurotrophic factor peptide-9.
Authors:Glazner GW,Camandola S,Mattson MP
Journal:Journal of neurochemistry
PubMed ID:10854252
Is rhodamine 123 an appropriate fluorescent probe to assess P-glycoprotein mediated multidrug resistance in vinblastine-resistant CHO cells?
Authors:Pétriz J, O'Connor JE, Carmona M, García-López J
Journal:Anal Cell Pathol
PubMed ID:9354229
Cellular drug resistance, which involves several mechanisms such as P-glycoprotein (P-gp) overexpression, kinetic and metabolic quiescence, or the increase in the intracellular levels of glutathione, limits the effectiveness of cancer treatment. It has been reported that functional assessment of the cationic dye rhodamin 123 (Rho123) efflux reveals accurately the drug-resistant ... More
Flow cytometric measurement of the production of reactive oxygen intermediate in activated rat mast cells.
Authors:Tsinkalovsky OR, Laerum OD
Journal:APMIS
PubMed ID:7520710
The production of reactive oxygen intermediates (ROI) in compound 48/80- or calcium ionophore A 23187- activated pleural or peritoneal mast cells was monitored using flow cytometry and the fluorescence indicator dihydrorhodamine 123 (a derivate of rhodamine 123). Mast cell degranulation and ROI production were estimated by flow cytometric fluorescence and ... More
A microtiter plate assay for screening antioxidant activity in extracts of marine organisms.
Authors:Dunlap W, Llewellyn L, Doyle J, Yamamoto Y
Journal:Mar Biotechnol (NY)
PubMed ID:14502401
A novel microtiter plate assay was developed to determine the total peroxyl radical-trapping activity of antioxidants extracted from marine organisms by measuring the inhibition rate of dye-substrate oxidation. We compared use of dihydrorhodamine-123, dihydrofluorescein, and dichlorodihydrofluorescein as reduced substrates for oxidation by peroxyl radicals generated from 2,2'-azobis(2-amidinopropane) dihydrochloride. The oxidation ... More
Direct real-time evaluation of nitration with green fluorescent protein in solution and within human cells reveals the impact of nitrogen dioxide vs. peroxynitrite mechanisms.
Authors:Espey MG, Xavier S, Thomas DD, Miranda KM, Wink DA
Journal:Proc Natl Acad Sci U S A
PubMed ID:11904413
'3-Nitrotyrosyl adducts in proteins have been detected in a wide range of diseases. The mechanisms by which reactive nitrogen oxide species may impede protein function through nitration were examined by using a unique model system, which exploits a critical tyrosyl residue in the fluorophoric pocket of recombinant green fluorescent protein ... More
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