二-2-ANEPEQ (JPW 1114)

引用和文献 (26)

Invitrogen™

二-2-ANEPEQ (JPW 1114)

ANEP 染料是因环境中的电位变化而发出荧光的分子。这些为快速响应探针，因其电子结构的变化及其荧光特性的变化而运行，以应对周围电场的变化。它们的光学响应速度足以检测可激发细胞（包括单神经元、心脏细胞和完整大脑）的瞬时（毫秒）电位变化。然而，它们的电位依赖性荧光变化量级通常很小；快速响应探针通常显示了了解更多信息

货号	数量
D6923 又称 D-6923	5 mg

货号 D6923

又称 D-6923

价格（CNY)

7,900.00

添加至购物车

价格（CNY)

7,900.00

添加至购物车

ANEP 染料是因环境中的电位变化而发出荧光的分子。这些为快速响应探针，因其电子结构的变化及其荧光特性的变化而运行，以应对周围电场的变化。它们的光学响应速度足以检测可激发细胞（包括单神经元、心脏细胞和完整大脑）的瞬时（毫秒）电位变化。然而，它们的电位依赖性荧光变化量级通常很小；快速响应探针通常显示了 2–10%/100 mV 的荧光变化。此外，这些染料在激发光谱中显示出电位依赖性变化，因此可使用激发比测量法对膜电位进行定量测定。

了解有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的更多信息›

电位敏感 ANEP 染料规格：
• 与模型磷脂膜结合的最大激发/发射波长为 ∼465/635 nm（但光谱特性高度依赖于环境）
• 与膜结合前不发荧光
• 阳离子分子；可溶于水（二-2-ANEPEQ 为水溶性 ANEP 染料）
• 通常通过显微注射将染料引入细胞
• 快速响应探针，适用于检测亚毫秒膜电位变化

电位探针应用
由于 K+、Na+ 和 Cl– 浓度梯度（通过主动转运过程维持），细胞质膜的跨膜电位通常约为 –70 mV（内部为负）。电位探针可提供检测这些离子易位的间接方法。

膜电位增加和减少—分别被称为膜超极化和去极化，—在许多生理过程（包括神经脉冲传播、肌肉收缩、细胞信号转导和离子通道门控）中发挥核心作用。电位探针是研究这些过程的重要工具。

查找更多 ANEP 染料
我们提供多种形式的 ANEP 染料。有关这些探针的更多信息，请查看 Molecular Probes™ 手册中的快速响应探针—第 22.2 节。

仅供科研使用。不可用于人或动物的治疗或诊断。

仅供科研使用。不可用于诊断程序。

检测方法荧光

数量5 mg

运输条件室温

子细胞定位细胞膜&脂质

颜色红外的

适用于（设备）荧光显微镜, 微孔板读数仪

产品类型ANEP 染料

Unit SizeEach

内容与储存

室温避光储存。

常见问题解答 (FAQ)

当我使用膜电位指示剂时，看到神经元周围出现了较高的背景，如何降低背景干扰？

如果使用我们的FluoVolt 膜电势试剂盒（货号F10488），该试剂盒包含一种背景抑制剂，可改善这一问题。对于其他指标剂，可以考虑使用BackDrop 背景抑制剂（货号R37603、B10511和B10512）。

快反应膜电位探针和慢反应膜电位探针有什么区别？

在周围电场的作用下结构变化的分子可用作检测瞬时（毫秒级）电位变化的快反应探针。慢反应染料则会进入去极化细胞，结合蛋白或膜。增强去极化会造成额外的染料流入，增强荧光强度；过极化的特征则是荧光强度下降。快反应探针通常用于完整心脏组织的电位活动成像，或测量药理刺激引起的膜电位变化。慢反应探针常用于探索线粒体功能和细胞活力。

你们提供哪些类型的膜电位指示剂？我该如何根据自己的试验选择？

膜电位指示剂选择指南请见此处（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html）。

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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引用和文献 (26)

引用和文献

Abstract

Dye screening and signal-to-noise ratio for retrogradely transported voltage-sensitive dyes.

Authors:Tsau Y, Wenner P, O'Donovan MJ, Cohen LB, Loew LM, Wuskell JP

Journal:J Neurosci Methods

PubMed ID:9007751

'Using a novel method for retrogradely labeling specific neuronal populations, we tested different styryl dyes in attempt to find dyes whose staining would be specific, rapid, and lead to large activity dependent signals. The dyes were injected into the ventral roots of the isolated chick spinal cord from embryos at ... More

Intrasarcomere [Ca2+] gradients and their spatio-temporal relation to Ca2+ sparks in rat cardiomyocytes.

Authors:Tanaka H, Sekine T, Kawanishi T, Nakamura R, Shigenobu K

Journal:J Physiol

PubMed ID:9490830

'1. Line-scan analyses of spontaneous Ca2+ sparks, non-propagating local rises in Ca2+ concentration, and the early phase of Ca2+ transients in cardiomyocytes were performed with a rapid-scanning laser confocal microscope (Nikon RCM8000) and fluo-3. 2. On electrical stimulation, points at which rise in Ca2+ began earliest were observed at regular ... More

Optical recording from cerebellar Purkinje cells using intracellularly injected voltage-sensitive dyes.

Authors:Kogan A, Ross WN, Zecevic D, Lasser-Ross N

Journal:Brain Res

PubMed ID:8624715

'We evaluated several techniques for their ability to record membrane potential changes with voltage-sensitive dyes introduced into CNS neurons in the brain slice preparation. Using a probe designed for intracellular application, JPW1114, we found that iontophoresis or pressure pulses could not push the lipophilic dye through electrodes whose resistance was ... More

Visual stimuli induce waves of electrical activity in turtle cortex.

Authors:Prechtl JC, Cohen LB, Pesaran B, Mitra PP, Kleinfeld D

Journal:Proc Natl Acad Sci U S A

PubMed ID:9207142

'The computations involved in the processing of a visual scene invariably involve the interactions among neurons throughout all of visual cortex. One hypothesis is that the timing of neuronal activity, as well as the amplitude of activity, provides a means to encode features of objects. The experimental data from studies ... More

Comparison of fluorescent voltage-sensitive dyes for multisite optical recording in hamster cerebral cortex by measurement of bicuculline-induced epileptiform events.

Authors:deBeer Zeiger J

Journal:Neuroimage

PubMed ID:9345545

'Two fluorescent voltage-sensitive dyes, RH795 and DI-2-ANEPPQ, were compared for in vivo multisite optical recording from the gustatory insular cortex of the golden Syrian hamster, the first reported use of DI-2-ANEPPQ in a mammalian brain preparation. The exposed cortex of the anesthetized hamster was stained with a 500 microM solution ... More

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