人血浆纤维蛋白原,Alexa Fluor™ 488 偶联物
人血浆纤维蛋白原,Alexa Fluor™ 488 偶联物
Invitrogen™

人血浆纤维蛋白原,Alexa Fluor™ 488 偶联物

Molecular Probes™ 纤维蛋白原偶联物通过将荧光染料分子连接至纯化人纤维蛋白原进行制备(每个纤维蛋白原分子连接大约 15 个染料分子),纯化偶联物以去除未反应的染料,然后冻干用于储存。荧光标记的纤维蛋白原已被证明是研究血小板活化以及随后的纤维蛋白原结合的有价值工具。例如,荧光素标记的纤维蛋白原已经用于通过流式细胞分析检测与活化的血小板结合的纤维蛋白原。人纤维蛋白原偶联物规格:•了解更多信息
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货号数量
F131915 mg
货号 F13191
价格(CNY)
4,421.00
Each
添加至购物车
数量:
5 mg
价格(CNY)
4,421.00
Each
添加至购物车
Molecular Probes™ 纤维蛋白原偶联物通过将荧光染料分子连接至纯化人纤维蛋白原进行制备(每个纤维蛋白原分子连接大约 15 个染料分子),纯化偶联物以去除未反应的染料,然后冻干用于储存。

荧光标记的纤维蛋白原已被证明是研究血小板活化以及随后的纤维蛋白原结合的有价值工具。例如,荧光素标记的纤维蛋白原已经用于通过流式细胞分析检测与活化的血小板结合的纤维蛋白原。

人纤维蛋白原偶联物规格:
• 标记(激发/发射波长):Alexa Fluor™ 488(约 495/519)
•光谱与荧光素相似,但信号的 pH 值依赖性较小,并且光稳定性更强
•冻干产品在缓冲液(例如 pH 值为 8.3 的碳酸氢钠)中溶解后使用
•通常使用荧光显微镜或流式细胞分析检测荧光


查找更多有关细胞粘附和受体结合的探针
请参阅《Molecular Probes™ 手册》中的用于细胞粘附、趋化作用、多药耐药性和谷胱甘肽的探针—第 15.6 节以及用于追踪受体结合和吞噬作用的探针—第 16.1 节,以获取有关这些探针的更多信息。

仅供研究使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于人或动物的治疗或诊断。
规格
标签类型Alexa Fluor 染料
产品线Alexa Fluor
蛋白质子类型纤维蛋白原
数量5 mg
运输条件室温
偶联物Alexa Fluor 488
形式Lyophilized
种属Human
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

引用和文献 (17)

引用和文献
Abstract
Assembly of a fibronectin matrix by adherent platelets stimulated by lysophosphatidic acid and other agonists.
Authors:Olorundare OE, Peyruchaud O, Albrecht RM, Mosher DF
Journal:Blood
PubMed ID:11418470
'Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are agonists of the endothelial differentiation gene (Edg) family of G-protein-coupled receptors. LPA and S1P are generated by platelet activation during blood coagulation. Both lipids induce assembly of exogenous fibronectin (FN) by fibroblasts. This study examined whether LPA and S1P stimulate binding and assembly ... More
Fibrinogen deposition at the postischemic vessel wall promotes platelet adhesion during ischemia-reperfusion in vivo.
Authors:Massberg S, Enders G, Matos FC, Tomic LI, Leiderer R, Eisenmenger S, Messmer K, Krombach F
Journal:Blood
PubMed ID:10572098
'Following ischemia-reperfusion (I/R), platelet adhesion is thought to represent the initial event leading to remodeling and reocclusion of the vasculature. The mechanisms underlying platelet adhesion to the endothelium have not been completely established. Endothelial cells rendered ischemic acquire a procoagulant phenotype, characterized by fibrinogen accumulation. Therefore, we evaluated whether fibrinogen ... More
Ultrathin self-assembled fibrin sheets.
Authors:O'Brien ET, Falvo MR, Millard D, Eastwood B, Taylor RM, Superfine R,
Journal:Proc Natl Acad Sci U S A
PubMed ID:19052234
'Fibrin polymerizes into the fibrous network that is the major structural component of blood clots and thrombi. We demonstrate that fibrin from three different species can also spontaneously polymerize into extensive, molecularly thin, 2D sheets. Sheet assembly occurs in physiologic buffers on both hydrophobic and hydrophilic surfaces, but is routinely ... More
Reprogramming cell shape with laser nano-patterning.
Authors:Vignaud T, Galland R, Tseng Q, Blanchoin L, Colombelli J, Théry M,
Journal:J Cell Sci
PubMed ID:22357956
Cell shape in vitro can be directed by geometrically defined micropatterned adhesion substrates. However conventional methods are limited by the fixed micropattern design, which cannot recapitulate the dynamic changes of the cell microenvironment. Here, we manipulate the shape of living cells in real time by using a tightly focused pulsed ... More
Kindlin-3 is essential for integrin activation and platelet aggregation.
Authors:Moser M, Nieswandt B, Ussar S, Pozgajova M, Fässler R,
Journal:Nat Med
PubMed ID:18278053
Integrin-mediated platelet adhesion and aggregation are essential for sealing injured blood vessels and preventing blood loss, and excessive platelet aggregation can initiate arterial thrombosis, causing heart attacks and stroke. To ensure that platelets aggregate only at injury sites, integrins on circulating platelets exist in a low-affinity state and shift to ... More