肝素荧光素偶联物

引用和文献 (42)

Invitrogen™

肝素荧光素偶联物

具有绿色荧光的荧光素肝素偶联物应该是研究这种粘多糖在细胞和组织中结合的一种有用工具。了解更多信息

货号	数量
H7482	1 mg

货号 H7482

价格（CNY)

4,447.00

添加至购物车

价格（CNY)

4,447.00

添加至购物车

具有绿色荧光的荧光素肝素偶联物应该是研究这种粘多糖在细胞和组织中结合的一种有用工具。

仅供科研使用。不可用于诊断程序。

标签类型经典染料

蛋白质子类型其他蛋白

数量1 mg

运输条件室温

偶联物Fluorescein

形式Lyophilized

Unit SizeEach

内容与储存

储存在冰箱（-5 至 -30°C）中并避光。

常见问题解答 (FAQ)

How can I dissolve Heparin, Fluorescein Conjugate (Cat. No. H7482)?

The molecular weight of Heparin, Fluorescein Conjugate (Cat. No. H7482) is ~18,000 and the information can be found on the Calcium Regulation—Section 17.2 page (see Data Table).

Heparin is quite soluble in water (10mg/ml) or aqueous buffer and you can prepare stock solutions of at least 1 mM using water as the solvent. Once dissolved, the stability of the nonsterile solution is very poor, so it should either be sterile filtered (0.2 µm) or an antibacterial (e.g., 0.06-1% benzyl alcohol) should be added. Heparin in solution should not be frozen, but is stable at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Biology Support Centers .

What is size of the Heparin, Fluorescein Conjugate (Cat. No. H7482)?

Heparin is an irregular aggregate of a 3,000 MW monomers, and they are low molecular weight heparins. They are purified by dialysis with a cut off of > 10 kDa and have an average of 18kDa.

Find additional tips, troubleshooting help, and resources within our Protein Biology Support Centers .

引用和文献 (42)

引用和文献

Abstract

Poly(beta-amino ester)s promote cellular uptake of heparin and cancer cell death.

Authors:Berry D, Lynn DM, Sasisekharan R, Langer R

Journal:Chem Biol

PubMed ID:15123243

'Heparin/heparan sulfate-like glycosaminoglycans (HSGAGs) are involved in diverse cellular processes in the extracellular matrix (ECM). The biological effect of HSGAGs depends on disaccharide content and physiological location within the ECM. HSGAGs are also brought into cells during membrane transcytosis and growth factor signaling while protein bound. We sought to probe ... More

Solid-phase synthesis of fluorescent heparin.

Authors:Radoff S, Danishefsky I

Journal:Anal Biochem

PubMed ID:7091663

Heparin binding is necessary, but not sufficient, for fibronectin aggregation. A fluorescence polarization study.

Authors:Bentley KL, Klebe RJ, Hurst RE, Horowitz PM

Journal:J Biol Chem

PubMed ID:3997865

'Analysis of parameters governing heparin binding to fibronectin indicates that heparin binding is a necessary, but insufficient, condition for fibronectin cryoprecipitation. Heparin binding to fibronectin is a rapid, readily reversible event which can occur under several conditions which prohibit fibronectin cryoprecipitation. While cryoprecipitation of fibronectin is abolished at temperatures in ... More

Binding and internalization of heparin by vascular smooth muscle cells.

Authors:Castellot JJ, Wong K, Herman B, Hoover RL, Albertini DF, Wright TC, Caleb BL, Karnovsky MJ

Journal:J Cell Physiol

PubMed ID:3930515

'Previous work from our laboratory has demonstrated that heparin specifically inhibits the proliferation of vascular smooth muscle cells in vivo and in vitro. In this paper, we examine the binding and mode of internalization of heparin by smooth muscle cells. For these studies, radiolabeled and fluoresceinated (FITC) heparin probes were ... More

Heparin binding by fibronectin module III-13 involves six discontinuous basic residues brought together to form a cationic cradle.

Authors:Busby TF, Argraves WS, Brew SA, Pechik I, Gilliland GL, Ingham KC

Journal:J Biol Chem

PubMed ID:7629186

'The thirteenth type III domain of fibronectin binds heparin almost as well as fibronectin itself and contains a so-called heparin-binding consensus sequence, Arg6-Arg7-Ala8-Arg9 (residues 1697-1700 in plasma fibronectin). Barkalow and Schwarzbauer (Barkalow, F.J., and Schwarzbauer, J.E. (1991) J. Biol. Chem. 266, 7812-7818) showed that mutation of Arg6-Arg7 in domain III-13 ... More

View all 肝素荧光素偶联物 citations