适用于进行显微镜检查的 Image-iT™ LIVE 红色和绿色半胱天冬酶细胞凋亡检测试剂盒
适用于进行显微镜检查的 Image-iT™ LIVE 红色和绿色半胱天冬酶细胞凋亡检测试剂盒
Actual product may vary
引用和文献 (15)
Invitrogen™

适用于进行显微镜检查的 Image-iT™ LIVE 红色和绿色半胱天冬酶细胞凋亡检测试剂盒

通过显微镜检查（包括荧光显微镜检查和 HCS）使用可报告多聚半胱天冬酶和半胱天冬酶-3/7 活性的 Image-iT LIVE 红色和绿色半胱天冬酶凋亡检测试剂盒检测细胞凋亡情况。
Have Questions?
更改视图buttonViewtableView
货号颜色酶标签或染料
I35102红色、绿色、蓝色半胱天冬酶 3/7SR-DEVD-FMK、SYTOX Green、Hoechst 33342
I35101红色、绿色、蓝色多聚半胱天冬酶SR-VAD-FMK、SYTOX Green、Hoechst 33342
I35104绿色、红色、蓝色多聚半胱天冬酶FAM-VAD-FMK、碘化丙啶 (PI)、Hoechst 33342
I35106绿色、红色、蓝色半胱天冬酶 3/7FAM-DEVD-FMK、碘化丙啶 (PI)、Hoechst 33342
货号 I35102
价格（CNY)
7,057.00
Each
添加至购物车
颜色:
红色、绿色、蓝色
酶:
半胱天冬酶 3/7
标签或染料:
SR-DEVD-FMK、SYTOX Green、Hoechst 33342
价格（CNY)
7,057.00
Each
添加至购物车
使用适用于进行显微镜检查（包括荧光显微镜检查和高内涵筛选 (HCS)）的 Image-iT LIVE 红色和绿色多聚半胱天冬酶以及半胱天冬酶-3 和半胱天冬酶-7 细胞凋亡检测试剂盒检测和评估半胱天冬酶活化、膜可透过性和细胞周期。Image-iT LIVE 红色和绿色半胱天冬酶检测试剂盒使用荧光半胱天冬酶抑制剂 (FLICA) 方法检测和报告半胱天冬酶活性，这是衡量细胞凋亡的指标。具体而言，Image-iT LIVE 红色和绿色半胱天冬酶检测试剂盒分别包含底物 SR-VAD-FMK 和 SR-DEVD-FMK（用于红色荧光）或 FAM-VAD-FMK 和 FAM-DEVD-FMK（绿色荧光），分别用于进行多聚半胱天冬酶和半胱天冬酶-3/7 检测。它们还包括 Hoechst 33342 和碘化丙啶染色剂，能够通过显微镜检查和 HCS 同时评估膜可透过性和细胞周期。
用于显微镜检查的 Image-iT LIVE 红色和绿色半胱天冬酶检测试剂盒采用了一种检测活性半胱天冬酶的新方法：这两种试剂盒均利用荧光半胱天冬酶抑制剂 (FLICA) 方法（亲和标记）。该试剂将一个可以与半胱氨酸发生共价反应的氟甲基酮 (FMK) 部分和一个半胱天冬酶特异性氨基酸序列相连接。不同的氨基酸识别序列用于检测不同的半胱天冬酶：缬氨酸-丙氨酸-天冬氨酸 (VAD) 用于检测多聚半胱天冬酶（包括半胱天冬酶-1、-3、-4、-5、-6、-7、-8 和 -9），天冬氨酸-谷氨酸-缬氨酸-天冬氨酸 (DEVD) 用于检测半胱天冬酶-3/7。连有一个磺酰罗丹明 (SR) 基团或羧基荧光素 (FAM) 基团，作为报告基团。

FLICA 试剂与已激活半胱天冬酶的反应中心通过识别序列相互作用，之后通过 FMK 基团与半胱氨酸共价连接。FLICA 抑制剂具有细胞渗透性但没有细胞毒性。未结合的 FLICA 分子扩散出细胞并被洗涤掉，其余的红色或绿色荧光信号可用于直接测定加入抑制剂时存在的活性半胱天冬酶的量。FLICA、碘化丙啶和 Hoechst 33342 试剂的近似激发峰和发射峰分别为 488⁄ nm/530 nm、535⁄ nm/6617 nm 和 350 nm/⁄461 nm。Image-iT LIVE 红色多聚半胱天冬酶、红色半胱天冬酶-3/7 绿色、绿色多聚半胱天冬酶和绿色半胱天冬酶-3/7 检测试剂盒中包括的检测试剂分别为 SR-VAD-FMK、SR-DEVD-FMK、FAM-VAD-FMK 和 FAM-DEVD-FMK。
仅供科研使用。不可用于诊断程序。
规格
颜色红色、绿色、蓝色
描述Image-iT LIVE 红色半胱天冬酶-3 和半胱天冬酶-7 检测试剂盒
酶半胱天冬酶 3/7
激发/发射SR-DEVD-FMK：550/595
SYTOX Green：504/523
Hoechst 33342：350/461
适用于（设备）荧光显微镜
标签类型其他标记或染料
标签或染料SR-DEVD-FMK、SYTOX Green、Hoechst 33342
反应次数25 次检测（标记体积为 300 µL）
产品线Image-iT
产品类型半胱天冬酶 3/7 试剂
数量1 kit
运输条件室温
储存要求避光储存在冷藏冰箱 (2–8°C) 中。
检测方法荧光
产品规格玻片
Unit SizeEach

引用和文献 (15)

引用和文献
Abstract
Endoplasmic Reticulum Stress and Apoptosis Contribute to the Pathogenesis of Dominantly Inherited Isolated GH Deficiency Due to GH1 Gene Splice Site Mutations.
Authors:Ariyasu D, Yoshida H, Yamada M, Hasegawa Y,
Journal:
PubMed ID:23736291
Dominantly inherited isolated GH deficiency is mainly caused by a heterozygous donor site mutation of intron 3 in the GH1 gene. An exon 3 deletion in GH (del32-71 GH) is produced from a mutant allele, whereas wild-type GH is produced from the other allele. Several studies have demonstrated a dominant ... More
Characterization of Differentiated SH-SY5Y as Neuronal Screening Model Reveals Increased Oxidative Vulnerability.
Authors:
Journal:J Biomol Screen
PubMed ID:26738520
JUND regulates pancreatic ß cell survival during metabolic stress.
Authors:Good AL, Cannon CE, Haemmerle MW, Yang J, Stanescu DE, Doliba NM, Birnbaum MJ, Stoffers DA
Journal:Mol Metab
PubMed ID:31023625
'In type 2 diabetes (T2D), oxidative stress contributes to the dysfunction and loss of pancreatic ß cells. A highly conserved feature of the cellular response to stress is the regulation of mRNA translation; however, the genes regulated at the level of translation are often overlooked due to the convenience of ... More
CD95-mediated apoptosis in Burkitt's lymphoma B-cells is associated with Pim-1 down-regulation.
Authors:Matou-Nasri S, Rabhan Z, Al-Baijan H, Al-Eidi H, Yahya WB, Al Abdulrahman A, Almobadel N, Alsubeai M, Al Ghamdi S, Alaskar A, AlBalwi M, Alzahrani M, Alabdulkareem I
Journal:Biochim Biophys Acta Mol Basis Dis
PubMed ID:27641442
'B-cells of the high-grade non-Hodgkin lymphoma Burkitt''s lymphoma (BL) overexpress survival oncoproteins, including the proviral integration site for Moloney murine leukaemia virus kinase (Pim)-1, and become apoptosis resistant. Activated death receptor CD95 after ligation with anti-CD95 monoclonal antibody (mAb) resulted in the regression of BL via induction of apoptosis, suggesting ... More
Impaired serotonin communication during juvenile development in rats diminishes adult sperm quality.
Authors:Díaz-Ramos J, Flores-Flores M, Ayala ME, Aragón-Martínez A
Journal:Syst Biol Reprod Med
PubMed ID:29788785
'Spermatogenesis and steroidogenesis are testicular functions regulated by gonadotrophins as well as other factors, including serotonin. Testicular serotonin acts as an autocrine regulator of testosterone secretion, but studies on its role in spermatogenesis and sperm quality are scarce. Here, we analyzed the effects of intratesticular inhibition of serotonin synthesis on ... More
View all 适用于进行显微镜检查的 Image-iT™ LIVE 红色和绿色半胱天冬酶细胞凋亡检测试剂盒 citations