S.N.A.P.™质粒 DNA 小提试剂盒
Product Image
Invitrogen™

S.N.A.P.™质粒 DNA 小提试剂盒

S.N.A.P.™小提试剂盒是一种质粒 DNA 纯化系统，旨在仅 25 分钟内产生纯质粒 DNA（图 1 和 2了解更多信息
Have Questions?
货号数量
K190001100 次制备
货号 K190001
价格（CNY)
4,652.00
飞享价
Ends: 31-Dec-2025
5,474.00
共减 822.00 (15%)
Each
添加至购物车
数量:
100 次制备
价格（CNY)
4,652.00
飞享价
Ends: 31-Dec-2025
5,474.00
共减 822.00 (15%)
Each
添加至购物车
S.N.A.P.™小提试剂盒是一种质粒 DNA 纯化系统，旨在仅 25 分钟内产生纯质粒 DNA（图 1 和 2）用于进行自动测序。纯质粒 DNA 可通过产生以下各项改善您的自动测序结果：

•更长的读段
• 更低的背景
• 更高的准确度
• 更强的信号峰

为确保获得较高质量，在自动测序反应中使用每个批次的 S.N.A.P.™小提试剂盒。必须至少获得 98.5% 准确序列的 450 个碱基。通常读取长度高达 650 个碱基。S.N.A.P.™小提试剂盒可从 1-3 ml 过夜培养物中获得高达 10 μg 纯小提质粒 DNA。
仅供科研使用。不可用于诊断程序。
规格
最终产品类型质粒 DNA
适用于（应用）下一代测序、体外转录、测序、克隆、转化
高通量能力不兼容高通量应用（手动）
反应次数100 次制备
制备规模< 100 μg（小规模）质粒 DNA
产品线S.N.A.P.™
产品类型质粒 DNA 小提试剂盒
数量100 次制备
样品类型细菌培养
靶标质粒 DNA
测试时间20 min 至 25 min
产品规格试剂盒
分离技术离心柱
Unit SizeEach
内容与储存
将 S.N.A.P.™小提离心柱、裂解缓冲液、结合缓冲液、洗涤缓冲液、4X 最终洗涤液和收集管储存在室温下。将重悬缓冲液、沉淀盐溶液和 RNase A 储存在 +4°C 下。所有试剂在适当储存时可保证稳定 6 个月。

常见问题解答 (FAQ)

What is the upper size limit for plasmid purification with the S.N.A.P. Miniprep Kit?

Bacmid DNA (>100 kbp) has been purified with the S.N.A.P. miniprep kit.

The PureLink HiPure Plasmid DNA Purification Kits (mini/midi/maxi scales) can also be used for large constructs. The HiPure kit manuals contain a modified protocol designed to help increase yields with these large plasmid isolations.

What are the compositions of the S.N.A.P. MiniPrep and MidiPrep Kit solutions?

The formulations are published in the manual for the S.N.A.P. MidiPrep kit, and are the same as the ones used in the MiniPrep kit.

What is the resin in the S.N.A.P. kits?

The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.

Why is LB medium recommended for growing up plasmid preps in the S.N.A.P. protocol?

The volumes of culture recommended in our S.N.A.P. kits assume an OD of no more than 2.0. If a rich medium is used, the volume processed should be reduced to avoid overloading the column.

Why can't I dissolve all of my DNA after precipitation?

Sometimes there is insoluble material (probably resin fines) in the precipitated DNA. This material is very easily removed with a 10 second spin. The presence of these particles does not reflect a poor preparation. Once the particles are removed, the DNA performs well in all applications.

View all S.N.A.P.™质粒 DNA 小提试剂盒 FAQs