S.N.A.P.™质粒 DNA 中提试剂盒
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Invitrogen™

S.N.A.P.™质粒 DNA 中提试剂盒

S.N.A.P.™MidiPrep 试剂盒是中等规模的质粒 DNA 纯化试剂盒,设计用于从 10-100 mL 过夜培养物中快速且简便地纯化纯质粒 DNA(图 1了解更多信息
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货号数量
K19100120 次制备
货号 K191001
价格(CNY)
6,018.21
飞享价
Ends: 31-Dec-2026
7,714.00
共减 1,695.79 (22%)
Each
添加至购物车
数量:
20 次制备
价格(CNY)
6,018.21
飞享价
Ends: 31-Dec-2026
7,714.00
共减 1,695.79 (22%)
Each
添加至购物车
S.N.A.P.™MidiPrep 试剂盒是中等规模的质粒 DNA 纯化试剂盒,设计用于从 10-100 mL 过夜培养物中快速且简便地纯化纯质粒 DNA(图 1)。S.N.A.P.™MidiPrep 程序仅需 40 分钟。您只需:

•用 S.N.A.P.™ 过滤柱裂解细胞并过滤碎片
• 将流过物加入 S.N.A.P.™DNA 结合柱
• 洗涤柱
• 用水或 TE 缓冲液洗脱纯、超螺旋质粒 DNA

用 S.N.A.P.™MidiPrep 试剂盒纯化的质粒 DNA 从柱上洗脱后可立即使用。无需沉淀。用 TE 或水洗脱 ∼200 ng/µL 质粒,适用于哺乳动物细胞转染等后续应用。典型得率见表 1。
仅供科研使用。不可用于诊断程序。
规格
最终产品类型质粒 DNA
适用于(应用)测序、克隆、转化、转染、体外转录
高通量能力不兼容高通量应用(手动)
制备规模100-200 µg(中等规模)质粒 DNA
产品线S.N.A.P.
产品类型质粒 DNA MidiPrep 试剂盒
数量20 次制备
样品类型细菌培养
规格中型胶
靶标质粒 DNA
测试时间40分钟
产品规格试剂盒
分离技术离心柱
Unit SizeEach
内容与储存
将 S.N.A.P.™中提过滤柱、S.N.A.P.™中提 DNA 结合柱、裂解缓冲液、结合缓冲液、洗涤缓冲液、4X 最终洗涤液储存在室温。将重悬缓冲液、沉淀盐和 RNase A 储存在 +4°C 下。妥善储存时,所有试剂均可保证 6 个月的稳定。

常见问题解答 (FAQ)

Which centrifuge is used at Thermo Fisher Scientific for the S.N.A.P. MidiPrep protocol?

The Sorval RT6000B table-top centrifuge is used at Thermo Fisher Scientific for the S.N.A.P. MidiPrep protocol. The low-speed spins are around 2,000 rpm and the high-speed spins are around 3,000 rpm. You may also use the Sorval RC-5B centrifuge with the SS-34 rotor. However, you might observe some spillage with this centrifuge.

What is the upper size limit for plasmid purification with the S.N.A.P. Miniprep Kit?

Bacmid DNA (>100 kbp) has been purified with the S.N.A.P. miniprep kit.

The PureLink HiPure Plasmid DNA Purification Kits (mini/midi/maxi scales) can also be used for large constructs. The HiPure kit manuals contain a modified protocol designed to help increase yields with these large plasmid isolations.

What are the compositions of the S.N.A.P. MiniPrep and MidiPrep Kit solutions?

The formulations are published in the manual for the S.N.A.P. MidiPrep kit, and are the same as the ones used in the MiniPrep kit.

What is the resin in the S.N.A.P. kits?

The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.

Why is LB medium recommended for growing up plasmid preps in the S.N.A.P. protocol?

The volumes of culture recommended in our S.N.A.P. kits assume an OD of no more than 2.0. If a rich medium is used, the volume processed should be reduced to avoid overloading the column.