NucRed™ Live 647 ReadyProbes™ 试剂
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NucRed™ Live 647 ReadyProbes™ 试剂
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NucRed™ Live 647 ReadyProbes™ 试剂

NucRed Live 647 ReadyProbes 试剂是一种明亮的远红外细胞通过性核染料,可用于活细胞或固定细胞。以方便使用的滴瓶内的室温稳定溶液形式提供。每毫升只需仅添加两滴对细胞进行染色。在 12.5X 浓度下,与1%了解更多信息
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货号数量
R371066个滴瓶
货号 R37106
价格(CNY)
2,910.00
飞享价
Ends: 31-Dec-2025
3,919.00
共减 1,009.00 (26%)
Each
添加至购物车
数量:
6个滴瓶
价格(CNY)
2,910.00
飞享价
Ends: 31-Dec-2025
3,919.00
共减 1,009.00 (26%)
Each
添加至购物车
NucRed Live 647 ReadyProbes 试剂是一种明亮的远红外细胞通过性核染料,可用于活细胞或固定细胞。以方便使用的滴瓶内的室温稳定溶液形式提供。每毫升只需仅添加两滴对细胞进行染色。

在 12.5X 浓度下,与1% DMSO、PowerLoad 增溶剂和 HBSS 缓冲液配制提供。也可以 DMSO 中浓度为250X 的 HCS 核遮盖深红色染色剂形式提供。

•无需稀释、称重或移液
• 方便使用的滴瓶—每毫升只需使用两滴
• 室温下稳定—可在您的使用范围或细胞培养区中保持便利
• 与 DNA 结合时可在638 nm 被激发,最大发射波长686 nm

查看其他用于细胞染色的 ReadyProbes 试剂
查看其他用于成像的核染色剂

细胞成像应用
远红外荧光发射使其成为与蓝色、绿色和红色荧光基团(如 Hoechst、GFP/FITC 和 Texas Red)结合使用的理想选择。

使用建议
• 使用 NucRed Live 647 ReadyProbes 试剂可以直接添加至完全培养基或缓冲溶液内的细胞中。
•在大多数情况下,2滴/mL 和15至30分钟孵育即可产生明亮的核染色;然而为实现较佳的染色强度,可以添加更多或更少的液滴。
•在大多数情况下,如果在成像前不洗涤细胞,染色强度随时间的增加而增加。
•可通过远红外滤光片检测,如 Cy5滤光片或适用于 EVOS 成像系统的 Cy5 Light Cube。
仅供科研使用。不可用于诊断程序。
规格
颜色远红外
检测方法荧光
染料类型细胞通透性
发射可见光
激发波长范围638⁄686
适用于(设备)共聚焦显微镜、荧光显微镜、流式细胞仪
形式液体
产品线NucRed,ReadyProbes
数量6个滴瓶
标签类型Fluorescent Dye
产品类型核酸染色剂
亚细胞定位细胞核/核仁
Unit SizeEach
内容与储存
6 × 2.5 mL 滴瓶

在 ≤25°C 下储存

常见问题解答 (FAQ)

How do I get rid of precipitates in NucRed Live 647 ReadyProbes Reagent (Cat. No. R37106)?

Briefly warm NucRed Live 647 ReadyProbes Reagent to 37 degrees C and vortex.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (23)

引用和文献
Abstract
Actin Dynamics Regulated by the Balance of Neuronal Wiskott-Aldrich Syndrome Protein (N-WASP) and Cofilin Activities Determines the Biphasic Response of Glucose-induced Insulin Secretion.
Authors:Uenishi E, Shibasaki T, Takahashi H, Seki C, Hamaguchi H, Yasuda T, Tatebe M, Oiso Y, Takenawa T, Seino S,
Journal:
PubMed ID:23867458
'Actin dynamics in pancreatic ß-cells is involved in insulin secretion. However, the molecular mechanisms of the regulation of actin dynamics by intracellular signals in pancreatic ß-cells and its role in phasic insulin secretion are largely unknown. In this study, we elucidate the regulation of actin dynamics by neuronal Wiskott-Aldrich syndrome ... More
Translational control of Nrf2 within the open reading frame.
Authors:Perez-Leal O, Barrero CA, Merali S,
Journal:
PubMed ID:23806685
'Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) is a transcription factor that is essential for the regulation of an effective antioxidant and detoxifying response. The regulation of its activity can occur at transcription, translation and post-translational levels. Evidence suggests that under environmental stress conditions, new synthesis of Nrf2 is required ... More
Neutrophil cathepsin G, but not elastase, induces aggregation of MCF-7 mammary carcinoma cells by a protease activity-dependent cell-oriented mechanism.
Authors:Yui S, Osawa Y, Ichisugi T, Morimoto-Kamata R,
Journal:
PubMed ID:24803743
'We previously found that a neutrophil serine protease, cathepsin G, weakens adherence to culture substrates and induces E-cadherin-dependent aggregation of MCF-7 human breast cancer cells through its protease activity. In this study, we examined whether aggregation is caused by degradation of adhesion molecules on the culture substrates or through an ... More
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation.
Authors:Gutiérrez-Caballero C, Burgess SG, Bayliss R, Royle SJ,
Journal:
PubMed ID:25596274
The interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3-ch-TOG onto mitotic spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule-binding ... More
Assessing telomere length using surface enhanced Raman scattering.
Authors:Zong S, Wang Z, Chen H, Cui Y,
Journal:
PubMed ID:25381775
Telomere length can provide valuable insight into telomeres and telomerase related diseases, including cancer. Here, we present a brand-new optical telomere length measurement protocol using surface enhanced Raman scattering (SERS). In this protocol, two single strand DNA are used as SERS probes. They are labeled with two different Raman molecules ... More