iBind™ Western 入门试剂盒，荧光检测

The iBind Western Starter Kit for fluorescent detection includes an iBind Western Device, iBind Cards, and an iBind Fluorescent Detection了解更多信息

货号	数量
SLF1002S	1 kit

货号 SLF1002S

价格（CNY)

1 kit

The iBind Western Starter Kit for fluorescent detection includes an iBind Western Device, iBind Cards, and an iBind Fluorescent Detection Solution Kit, so you can get started with automated blot processing right away.

The iBind Western Device is a small benchtop device that automates western blot processing (immunodetection). It applies mechanical pressure to an iBind Card to generate sequential lateral flow (SLF) of immunodetection solutions for the blocking, antibody binding, and washingsteps. The glass fiber matrix of the iBind Card allows for homogenous and consistent flow of the solutions over the membrane, increasing the antigen-antibody interaction.

The iBind Western Device allows all solutions to be prepared and loaded in the device at the start of the procedure; all subsequent steps proceed automatically through SLF action—no electricity or batteries required. Blots are ready for the final detection steps in approximately 2.5 hours.

Learn more about iBind western systems ›

The iBind Western Device works exclusively with iBind Cards, and the iBind Solution Kit or iBind Fluorescent Detection (FD) Solution Kit to process one mini-blot.

仅供科研使用。不可用于诊断程序。

数量1 kit

运输条件室温

容量1次小型印迹

检测方法荧光

适用于（应用）蛋白免疫印迹

膜兼容性硝酸纤维素、PVDF

产品线iBind

运行时间∼2.5小时

通量1次小型印迹

类型启动套装

Unit Size1 kit

内容与储存

1 台 iBind Western 设备、10 张 iBind 卡（在室温下储存）、1 个 iBind 荧光检测 (FD) 溶液试剂盒（在 2 至 8°C 下储存）

常见问题解答 (FAQ)

Do you offer additional years of service for the iBind Western Device?

No, we do not provide service contracts or extended warranties for these devices. The warranty is limited to 1 year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.