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Invitrogen
Human Siglec-2 (CD22) His-tag Recombinant Protein
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Product Details
RP-88167
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Met1-Arg687
Tag
His-tag
Molecular weight
76.6 kDa
Class
Recombinant
Type
Protein
Purity
≥ 95 % as determined by SDS-PAGE
Activity
Measured by its binding ability in a functional ELISA. Immobilized human CD22 at 0.02 µg/ml (100 µL/well) can bind anti-human CD22 Mab with a linear range of 0.2-50 ng/mL.
Conjugate
Form
Lyophilized
Amount
1 mg
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (4000 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
CD22, also known as BL-CAM, is a type I transmembrane glycoprotein composed of two polypeptide chains, CD22alpha and CD22beta, with molecular weights of 130 and 140 kDa, respectively. These chains are produced by alternative splicing of the CD22 gene. CD22 is prominently expressed on mature B cells and B cell lymphomas, including hairy cell leukemia, diffuse large B-cell lymphoma, and nodular lymphocyte predominance Hodgkin's lymphoma, but is negative in classical Hodgkin's lymphoma. The extracellular portion of CD22 contains seven Ig-like domains that preferentially bind alpha2,6-linked sialic acid moieties found on epithelial, endothelial, B, and T cells. This binding can be masked by cis interactions with sialic acids on the same cell surface. CD22 expression is limited to late stages of B-cell differentiation, making it useful for phenotyping mature leukemias. Intracellularly, CD22 features six tyrosine residues within immunotyrosine-based inhibitory motifs (ITIM) and activation-like motifs. These residues are phosphorylated upon B-cell receptor engagement, allowing CD22 to regulate B-cell receptor signaling. CD22 participates in positive regulation through interactions with Src family tyrosine kinases and acts as an inhibitory receptor by recruiting cytoplasmic phosphatases via SH2 domains, which block signal transduction through dephosphorylation of signaling molecules. CD22's role in both positive and negative regulation of B-cell signaling, along with its specific expression pattern, makes it a valuable marker for antibody customers interested in B-cell-related research and diagnostics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell receptor CD22; B-lymphocyte cell adhesion molecule; B-lymphocyte cell adhesion molecule (BL-CAM); BL-CAM; CD22; CD22 antigen; FLJ22814; Lectin 2; Leu-14; MGC130020; Sialic acid-binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2 (Siglec-2); Siglec-2; T-cell surface antigen Leu-14; unnamed protein product
Gene Aliases: CD22; SIGLEC-2; SIGLEC2
UniProt ID: (Human) P20273
Entrez Gene ID: (Human) 933
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