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Invitrogen
Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-eFluor™ 710, eBioscience™
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产品信息
46-4031-82
应用
建议稀释比
流式细胞分析 (Flow)
Assay-Dependent
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Rat
/ IgG2b, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
eB149/10H5
偶联物
激发/发射光谱
482/708 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported: PerCP-eFluor® 710 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.
Applications Tested: PerCP-eFluor® 710 Rat IgG2b Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells.Use at the same concentration as experimental antibody.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
