Identifyn

PARP1 SRM Recombinant Rabbit Monoclonal Antibody (BLR018M), Alexa Fluor™ 594, Identifyn™

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说明书
实验方案
问题与答案
技术支持
说明书
实验方案
问题与答案
技术支持
说明书
实验方案
问题与答案
技术支持
PARP1 SRM Recombinant Rabbit Monoclonal Antibody (BLR018M), Alexa Fluor™ 594, Identifyn™

产品信息

DC-000012

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:250-1:1,000
-

免疫细胞化学 (ICC/IF)

1:250-1,000
-
产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

ExpiCHO

分类

Recombinant Monoclonal

类型

Antibody

克隆号

BLR018M

抗原

Immunogen was a peptide representing the cleaved N-terminal of human Poly [ADP-ribose] polymerase 1 PARP1, a region between residue 210 and 265, referencing both Uniprot ID P09874 and Gene ID 142.
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偶联物

Alexa Fluor™ 594 Alexa Fluor™ 594 Alexa Fluor™ 594

激发/发射光谱

590/618 nm 查看光谱 spectra

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 10% glycerol

内含物

0.02% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.

Recommended dilution for traditional microscopy:

Widefield - 1:250-1:1,000

Widefield SIM - 1:250-1:1,000

Confocal - 1:250-1:1,000

Recommended dilution for Super Resolution microscopy:

SIM (Structured Illumination Microscopy) - 1:1,000

Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:100-1:1,000

Detailed protocols can be found below under Documents & Downloads.

靶标信息

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; Protein poly-ADP-ribosyltransferase PARP1

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基因别名: ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PARP1; PPOL

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UniProt ID: (Human) P09874

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Entrez Gene ID: (Human) 142

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功能
DNA binding DNA ligase (ATP) activity NAD+ ADP-ribosyltransferase activity protein binding transcription factor binding zinc ion binding enzyme binding protein kinase binding identical protein binding histone deacetylase binding poly(A) RNA binding protein N-terminus binding NAD binding R-SMAD binding DNA metabolism protein
参与通路
negative regulation of transcription from RNA polymerase II promoter nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding double-strand break repair via homologous recombination lagging strand elongation DNA repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion double-strand break repair transcription from RNA polymerase II promoter protein ADP-ribosylation cellular response to DNA damage stimulus mitochondrion organization transforming growth factor beta receptor signaling pathway positive regulation of cardiac muscle hypertrophy protein autoprocessing protein sumoylation signal transduction involved in regulation of gene expression macrophage differentiation mitochondrial DNA metabolic process cellular response to insulin stimulus nucleotide-excision repair, DNA incision cellular response to oxidative stress protein modification process DNA damage response, detection of DNA damage mitochondrial DNA repair positive regulation of transcription from RNA polymerase II promoter DNA ligation involved in DNA repair positive regulation of SMAD protein import into nucleus protein poly-ADP-ribosylation global genome nucleotide-excision repair regulation of cellular protein localization negative regulation of telomere maintenance via telomere lengthening ATP generation from poly-ADP-D-ribose positive regulation of transcription regulatory region DNA binding
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