Oligonucleotide HPLC and UHPLC Columns

Overview

Performance and confidence at the throughput you need for oligonucleotide analysis

When purity and characterization are critical, rely on our anion-exchange and reversed-phase column chemistries. For over 30 years, the Thermo Scientific DNAPac family of columns has been the standard for high-resolution UHPLC separation of oligonucleotides, delivering consistent, high-quality results. Building on this legacy, Thermo Scientific SurePac Oligo RP MDi columns are engineered for high-resolution reversed-phase separations under elevated pH and temperature conditions, helping reduce secondary interactions and improve reproducibility. Together, these solutions deliver the confidence and throughput required for demanding oligonucleotide analysis workflows.

Biomolecule HPLC and UHPLC columns

Recommended HPLC columns for oligonucleotide analysis

	DNAPac RP HPLC Columns	DNAPac PA200 & DNAPac PA200RS HPLC Columns	DNASwift HPLC Columns	SurePac Oligo RP MDi
✓ Compatible with LC-UV and LC-MS/MS ✓ Offer ion-pair reversed phase separations of nucleic acid mixtures ✓ Oligonucleotide samples from siRNA to mRNA easily resolve on this polymer chemistry ✓ Deliver outstanding separations	✓ Compatible with LC-UV ✓ Strong anion-exchange columns for n, n-1, and n+1 separations of oligonucleotide samples ✓ Offer orthogonal separation to reversed phase columns ✓ Separate oligonucleotide samples by size and charge	✓ A monolithic column designed for strong anion-exchange purification of oligonucleotide samples using analytical HPLC ✓ High loadability, with slightly less resolution than our analytical columns	✓ Compatible with LC-UV and LC-MS/MS ✓ Bio-inert flow path minimizes secondary interactions and adsorption ✓ Engineered for compatibility with elevated pH and temperature conditions ✓ High-resolution separation of oligonucleotides, from small siRNA to large mRNA species ✓ Consistent retention and peak shape for reliable quantitation ✓ Scalable performance across a wide range of oligonucleotide lengths and complexities
	Order now	Order now	Order now	Order now

Oligonucleotide HPLC & UHPLC Columns

DNAPac RP HPLC Columns

DNAPac PA200 & DNAPac PA200RS HPLC Columns

DNASwift HPLC Columns

SurePac Oligo RP MDi

✓ Compatible with LC-UV and LC-MS/MS

✓ Offer ion-pair reversed phase separations of nucleic acid mixtures

✓ Oligonucleotide samples from siRNA to mRNA easily resolve on this polymer chemistry

✓ Deliver outstanding separations

✓ Compatible with LC-UV

✓ Strong anion-exchange columns for n, n-1, and n+1 separations of oligonucleotide samples

✓ Offer orthogonal separation to reversed phase columns

✓ Separate oligonucleotide samples by size and charge

✓ A monolithic column designed for strong anion-exchange purification of oligonucleotide samples using analytical HPLC

✓ High loadability, with slightly less resolution than our analytical columns

✓ Compatible with LC-UV and LC-MS/MS

✓ Bio-inert flow path minimizes secondary interactions and adsorption

✓ Engineered for compatibility with elevated pH and temperature conditions

✓ High-resolution separation of oligonucleotides, from small siRNA to large mRNA species

✓ Consistent retention and peak shape for reliable quantitation

✓ Scalable performance across a wide range of oligonucleotide lengths and complexities

Order now

Oligonucleotide analysis requires more than just the right column. From bioinert flow paths to optimized system performance, every component plays a role in achieving accurate, reproducible results. Explore our complete solutions designed to support your workflow from method development through QC.

Learn more about oligonucleotide analysis solutions ›