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Invitrogen
CD140a (PDGFRA) Monoclonal Antibody (APA5), Functional Grade, eBioscience™
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CD140a (PDGFRA) Antibody (16-1401-82) in Flow
Staining of NIH/3T3 cells with 0.5 µg of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.5 µg of Anti-Mouse CD140a (PDGF Receptor a) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
产品信息
16-1401-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse,
Rat
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
APA5
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.
Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistology staining of frozen tissue sections. It has also been reported for blocking ligand binding.
Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of the NIH/3T3 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF alpha chain; PDGF Receptor alpha; PDGF subunit A; PDGF-1; PDGF-R-alpha; pdgfr-a; PDGFR-alpha; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor alpha
基因别名: AI115593; CD140a; Pdgfr-2; Pdgfra
UniProt ID: (Mouse) P26618
Entrez Gene ID: (Mouse) 18595
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
platelet-derived growth factor alpha-receptor activity
vascular endothelial growth factor-activated receptor activity
platelet-derived growth factor receptor binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein complex binding
vascular endothelial growth factor binding
protein homodimerization activity
phosphatidylinositol 3-kinase binding
platelet-derived growth factor binding
transmembrane signal receptor
luteinization
in utero embryonic development
hematopoietic progenitor cell differentiation
protein phosphorylation
chemotaxis
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cytosolic calcium ion concentration
multicellular organism development
estrogen metabolic process
positive regulation of cell proliferation
female gonad development
anatomical structure morphogenesis
organ morphogenesis
negative regulation of platelet activation
positive regulation of phospholipase C activity
phosphorylation
cell migration
peptidyl-tyrosine phosphorylation
signal transduction involved in regulation of gene expression
extracellular matrix organization
lung development
adrenal gland development
positive regulation of cell migration
male genitalia development
Leydig cell differentiation
cellular response to reactive oxygen species
platelet-derived growth factor receptor-alpha signaling pathway
cellular response to platelet-derived growth factor stimulus
positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
wound healing
odontogenesis of dentin-containing tooth
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of DNA replication
protein autophosphorylation
platelet-derived growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of fibroblast proliferation
embryonic digestive tract morphogenesis
embryonic cranial skeleton morphogenesis
skeletal system morphogenesis
regulation of chemotaxis
cardiac myofibril assembly
palate development
face morphogenesis
cell chemotaxis
retina vasculature development in camera-type eye
positive regulation of ERK1 and ERK2 cascade
platelet aggregation
cellular response to amino acid stimulus
metanephric glomerular capillary formation
regulation of mesenchymal stem cell differentiation
