Invitrogen

CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), Super Bright™ 645, eBioscience™

Product Image Gallery

CD90.1 (Thy-1.1) Antibody in Flow Cytometry (Flow) — Staining of rat splenocytes with Anti-Rat CD3 APC (Product # 17-0030) and 0.25 µg of Mouse IgG2a kappa Isotype Control Super Bright 645 (Product # 64-4724) (left) or 0.25 µg of CD90-1 (Thy-1-1) Monoclonal Antibody, Super Bright 645 (right). Total viable cells were used for analysis.

CD90.1 (Thy-1.1) Antibody in Immunocytochemistry (ICC/IF) — Figure 3 Expression of CD90 marker in pituispheres and MSC. Immunofluorescence images of PA02, PA03, and PA05 (A) MSC and (B) pituispheres stained for CD90 (green). Isotype controls were used as negative control. Cell nuclei were counterstained with DAPI (blue). Scale bar, 13 mum.

CD90.1 (Thy-1.1) Antibody in Immunohistochemistry (IHC) — Fig. 1 a An inverted microscope micrograph from primary culture of mesenchymal stem cells (black arrow). b Fluorescent microscopic image demonstrating fluorescence of MSCs labeled with GFP 2 weeks after implantation. c Flow cytometry analysis of surface antigens of MSCs (blue is the isotype, green is the detected antigen); CD34 0.7%, CD90 92.5%, and CD105 98.7%. d Osteogenesis differentiation stained with Alizarin red stain and its control. e Adipogenesis differentiation stained with Oil Red O stain and its control. f Chondrogenesis differentiation stained with Alcian blue stain and its control. g CD105 immuno-expression in group VI. h CD105 immuno-expression in group V. i Histogram representing the mean area percentage of CD105 immunoreaction in treated groups (group IV and V). The significant differences against chemo-ablated group indicated by a ""*"" for p < 0.05 and a ""**"" for p < 0.01. Data are shown as mean +- S.E.M, n = 7

产品信息

64-0900-82

应用	建议稀释比	已发表文章
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫细胞化学 (ICC/IF)	-	查看1篇已发表文章 1篇已发表文章
流式细胞分析 (Flow)	0.5 µg/test	查看9篇已发表文章 9篇已发表文章

产品规格
种属反应	Mouse, Rat
已发表种属	Not Applicable
宿主/亚型	Mouse / IgG2a, kappa
推荐的同型对照	Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 645, eBioscience™
分类	Monoclonal
类型	Antibody
克隆号	HIS51
偶联物	Super Bright™ 645 APC APC-eFluor 780 Biotin eFluor 450 FITC PE PE-Cyanine5 PE-Cyanine7 PerCP-Cyanine5.5 Super Bright 436 Super Bright 600 Unconjugated
激发/发射光谱	414/645 nm 查看光谱
形式	Liquid
浓度	0.2 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2, with BSA
内含物	0.09% sodium azide
保存条件	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2717097

产品详细信息

Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.

Applications Reported: This HIS51 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIS51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Sustained release of exosomes loaded into polydopamine-modified chitin conduits promotes peripheral nerve regeneration in rats.

Neural regeneration research

Li C,Liu SY,Zhang M,Pi W,Wang B,Li QC,Lu CF,Zhang PX

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 64-0900-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2022

Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium.

Frontiers in bioengineering and biotechnology

Guo Q,Chang Y,Li J,Zhou C,Huang R,Yang X,Liu G,Liang X

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 64-0900-82) in Flow Cytometry

Sat Jul 16 00:00:00 EDT 2022

Binary outcomes of enhancer activity underlie stable random monoallelic expression.

eLife

Kissiov DU,Ethell A,Chen S,Wolf NK,Zhang C,Dang SM,Jo Y,Madsen KN,Paranjpe I,Lee AY,Chim B,Muljo SA,Raulet DH

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 64-0900-82) in Flow Cytometry

Thu May 26 00:00:00 EDT 2022

Propofol suppresses adipose-derived stem cell progression via PI3K/AKT-Wnt signaling pathway.

BMC anesthesiology

Yin G,Wang J,Zhong Y,Wu W

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 64-0900-82) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2022

Bone marrow-derived mesenchymal stem cells combined with gonadotropin therapy restore postnatal oogenesis of chemo-ablated ovaries in rats via enhancing very small embryonic-like stem cells.

Stem cell research & therapy

Ebrahim N,Al Saihati HA,Shaman A,Dessouky AA,Farid AS,Hussien NI,Mostafa O,Seleem Y,Sabry D,Saad AS,Emam HT,Hassouna A,Badr OAM,Saffaf BA,Forsyth NR,Salim RF

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 64-0900-82) in Immunohistochemistry

Mon Sep 27 00:00:00 EDT 2021

Adipose mesenchymal stem cells combined with platelet-rich plasma accelerate diabetic wound healing by modulating the Notch pathway.

Stem cell research & therapy

Ebrahim N,Dessouky AA,Mostafa O,Hassouna A,Yousef MM,Seleem Y,El Gebaly EAEAM,Allam MM,Farid AS,Saffaf BA,Sabry D,Nawar A,Shoulah AA,Khalil AH,Abdalla SF,El-Sherbiny M,Elsherbiny NM,Salim RF

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 64-0900-82) in Flow Cytometry

Tue Jul 13 00:00:00 EDT 2021

Macrophage polarization by MSC-derived CXCL12 determines tumor growth.

Cellular & molecular biology letters

Babazadeh S,Nassiri SM,Siavashi V,Sahlabadi M,Hajinasrollah M,Zamani-Ahmadmahmudi M

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 64-0900-82) in Flow Cytometry

Sat Jun 26 00:00:00 EDT 2021

Pituispheres Contain Genetic Variants Characteristic to Pituitary Adenoma Tumor Tissue.

Frontiers in endocrinology

Peculis R,Mandrika I,Petrovska R,Dortane R,Megnis K,Nazarovs J,Balcere I,Stukens J,Konrade I,Pirags V,Klovins J,Rovite V

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 64-0900-82) in Immunocytochemistry

Mon May 10 00:00:00 EDT 2021

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 64-0900-82) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

Effects of bone marrow‑derived mesenchymal stem cell transplantation on chronic obstructive pulmonary disease/obstructive sleep apnea overlap syndrome in rats.

Molecular medicine reports

Chen M,Huang Z,Bi H,Pan X,He J,He L,He X,Du J,Zhou K,Wang L,Wang Q,Guo X,Jin Z

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 64-0900-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro.

Molecular medicine reports

Hu Y,Zhang Y,Tian K,Xun C,Wang S,Lv D

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 64-0900-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

生物信息学

蛋白别名: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; thy-1 glycoprotein; Thy-1 membrane glycoprotein; Thy-1 protein; thymus cell antigen 1, theta; Thymus cell surface antigen

基因别名: CD7; CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

UniProt ID: (Mouse) P01831, (Rat) P01830

Entrez Gene ID: (Mouse) 21838, (Rat) 24832

分子生物学功能: cell adhesion molecule

功能

angiogenesis negative regulation of protein kinase activity cytoskeleton organization single organismal cell-cell adhesion negative regulation of cell migration positive regulation of GTPase activity retinal cone cell development protein autophosphorylation focal adhesion assembly negative regulation of axonogenesis T cell receptor signaling pathway negative regulation of T cell receptor signaling pathway positive regulation of T cell activation positive regulation of release of sequestered calcium ion into cytosol negative regulation of apoptotic process mast cell activation negative regulation of fibroblast proliferation positive regulation of peptidyl-tyrosine phosphorylation

参与通路

GTPase activator activity integrin binding enzyme binding protein kinase binding GPI anchor binding protein binding

CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), Super Bright™ 645, eBioscience™

产品信息

64-0900-82

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Your items have has been added!