Protein Crosslinking Solutions

End-to-end solution for your MS-based crosslinking structural analysis

Every part of our workflow has been optimized to generate maximum insight, removing the guesswork out of choosing the appropriate configuration for your experiments. We offer the complete solution plus a full range of resources to assist you throughout the entire workflow.

Sample separation for crosslinking research

Vanquish Neo UHPLC System for experiments that require high reproducibility and sensitivity with low carryover

For high-sensitivity LC-MS applications, Thermo Scientific Vanquish Neo UHPLC System flawlessly integrates with the industry-leading Thermo Scientific mass spectrometry portfolio and ESI sources.

EASY-Spray PepMap Neo UHPLC Columns

Thermo Scientific EASY-Spray PepMap Neo UHPLC Columns feature easy connectivity, high reproducibility, and excellent separations. Neo columns provide 1500 bar pressure capability, improved column-to-column consistency, and increased efficiency. The Click-and-Spray format of EASY-Spray columns enables perfect connections to our mass spectrometers every time.

FAIMS Pro Duo interface for experiments that require extended dynamic range

For the generation of deeper sequence coverage, allowing for the detection of crosslinked peptides present in low abundance, Thermo Scientific FAIMS Pro Duo interface offers improved selectivity and sensitivity, even at very low picogram loading levels. The advanced high field asymmetric ion mobility technology employed by this interface enables precise targeting of peptides within the ion cloud, while effectively maintaining the cleanliness of the mass spectrometer and ensuring the purity of the spectra.

Mass spectrometers for crosslinking research

The Thermo Scientific Orbitrap family of mass spectrometers offers reliable proven technology to support MS-based crosslinking analysis with accuracy and precision.

 

Differentiating features of all Orbitrap instruments

  • Automatic gain control (AGC) to enable consistent spectral quality and quantitative accuracy from picogram to microgram loading levels without user intervention
  • Normalized collision energy to enable reproducible fragmentation efficiency of all precursors without optimization within and across sample cohorts
  • Complete mass range (m/z) of MS/MS scans to enable unambiguous identifications of crosslinking sites
  • Unparalleled spectral quality and intra-spectrum dynamic range enabled by the Orbitrap mass analyzer

Orbitrap Astral Mass Spectrometer for experiments that require unprecedented depth

Benefit from a new level of depth in characterization of the proteins, with industry leading sensitivity of analysis, while maintaining the ability to analyze samples of high dynamic range. Thermo Scientific Orbitrap Astral Mass Spectrometer supports samples of low amount (<pg) and high amount (>ug) without compromise or saturation of analysis.

Orbitrap Ascend Structural Biology Mass Spectrometer for experiments that require ultimate characterization and versatility

Exceptional versatility of analysis to enable the ultimate characterization of crosslinked samples. The Thermo Scientific Orbitrap Ascend Structural Biology Tribrid Mass Spectrometer Real-Time Library Search feature triggers high-resolution MS2 scans based on intensity ratios, significantly improving the identification of crosslinked peptides.

Orbitrap Exploris 480 Mass Spectrometer for experiments that require Orbitrap accuracy and enhanced selectivity for accurate quantitation

Experience proven reliability and spectral quality for accurate identification of crosslinked sites. Thermo Scientific Orbitrap Exploris 480 Mass Spectrometer offers versatile operation to support data-dependent or data-independent acquisitions for discovery and quantitation with high resolution accurate mass.

Data analysis for crosslinking research

Proteome Discoverer Software

Achieve robust analysis of crosslinking data. Thermo Scientific Proteome Discoverer Software provides fully integrated crosslink peptide search engine for protein crosslinking MS analysis, facilitating annotation of crosslinked peptides, allowing for the identification of both inter- and intra-crosslinked peptides, as well as mono-adducts. In addition, it offers accurate TMT and label-free quantification of crosslinked peptides with cross-linking protocols for studies involving protein-protein and protein-RNA/DNA interactions.

For Research Use Only. Not for use in diagnostic procedures.