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StemPro MSC SFM XenoFree
Xeno-free media specifically formulated for the growth and expansion of human mesenchymal stem cells (hMSC) and human adipose-derived stem cells (hADSC)
StemPro MSC SFM XenoFree medium is a fully defined, serum-free and xeno-free medium designed for the isolation, culture, and expansion of hMSCs and hADSCs. It supports growth over multiple passages while preserving MSC morphology, phenotype, and trilineage mesoderm differentiation potential.
When paired with CELLstart Substrate, StemPro enables a xeno-free MSC culture workflow from primary isolation through expansion. The cGMP-compliant formulation offers lot-to-lot consistency, traceability, and regulatory-friendly performance for advanced stem cell research applications.
In practice, StemPro MSC SFM XenoFree offers:
- Excellent performance under serum-free, xeno-free conditions, helping reduce variability associated with animal-derived components.
- Robust MSC expansion while maintaining the spindle-shaped morphology typical of healthy MSCs.
- Stable MSC identity over time, including surface marker expression and normal gene expression profiles.
- Integration into a complete xeno-free workflow when used with CELLstart Substrate for primary isolation and expansion.
- Functional potency required for downstream mesoderm-lineage applications, including adipogenic, chondrogenic, and osteogenic differentiation.
Supports hMSC expansion
Expansion of hMSCs using StemPro MSC SFM XenoFree on CELLstart substrate-coated flasks is comparable to classical medium (DMEM + 10% FBS) (Figure 1).
Figure 1. Expansion of MSCs in StemPro MSC SFM XenoFree. Human Bone Marrow-derived MSCs (BM-MSCs) cultured in StemPro MSC SFM XenoFree on CELLstart-coated flasks reveal a similar growth rate compared to DMEM + 10% MSC-Qualified FBS with long-term multi-passage capability.
Better quality cells—spindle-shaped morphology
hMSCs grown in StemPro MSC SFM XenoFree medium on CELLstart substrate-coated flasks exhibit a less flattened, spindle-shaped morphology (Figure 2).
Figure 2. Human BM-MSC morphology. BM-MSCs expanded in StemPro MSC SFM XenoFree display a conserved spindle-shaped morphology after 9 passages compared to DMEM + 10% MSC-Qualified FBS. Images were captured using a 10X objective.
Maintains multilineage differentiation potential
hMSCs cultured in StemPro MSC SFM XenoFree retain their tri-lineage mesoderm differentiation potential (Figure 3).
Figure 3. Multilineage differentiation. Human MSCs expanded for 5 passages in StemPro MSC SFM XenoFree retained multipotency as shown by the ability to differentiate into adipocytes, osteoblasts, and chondrocytes. Staining: adipogenesis = Oil Red O (Day 14); chondrogenesis = Alcian blue (Day 14) osteogenesis = alkaline phosphatase (Day 21). Images were captured using a 10X objective.
Primary isolation of hMSCs
Purified bone marrow mononuclear cells cultured in StemPro MSC SFM XenoFree supplemented with low level pooled human AB serum (2.5%) supported primary BM-MSC expansion with continual propagation (after Passage 0) under serum- and xeno-free conditions (Figure 4).
Figure 4. Primary human BM-MSC expansion. Ficoll-purified BM-MNCs cultured in StemPro MSC SFM XenoFree (on CELLstart-coated flasks) supplemented with low level pooled human AB serum (2.5%) supported primary BM-MSC expansion with continual propagation (after P0) under serum- and xeno-free conditions.
Full MSC isolation protocol
For detailed instructions on serum-free, xeno-free MSC isolation and expansion—including CELLstart coating, seeding, feeding, and passaging—refer to our protocol.
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For Research Use Only. Not for use in diagnostic procedures.
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