Stay focused on your critical work to achieve clear and accurate imaging results. Our breadth of imaging products from plastics and media to probes and dyes are optimized for quality to help control the experiment from start to finish and deliver reliable results.
Alexa Fluor Plus 405 secondary antibodies
New Alexa Fluor Plus 405 conjugated secondary antibodies are now available. Alexa Fluor Plus 405 conjugates are optimized for the highest signal to noise ratio in immunocytochemistry and immunofluorescence applications, with up to 4x the brightness of other 405 conjugates. Continue to expand your multiplexed imaging capabilities by using these bright conjugates to identify low abundance targets, leaving other channels for a different fluorescent imaging readout.
Learn more about Alexa Fluor Plus 405 secondary antibodies
Cell health and function assays
Understanding cellular function through imaging is a valuable tool for biology research. Thermo Fisher Scientific offers a broad range of cell function assays and reagents to elucidate key cellular processes including apoptosis, cell proliferation, viability and cytotoxicity, oxidative stress, and internalization processes like phagocytosis and endocytosis, as well as indicators for ion homeostasis.
Fluorescent conjugated secondary antibodies are a valuable tool for identifying cellular proteins in fluorescent imaging, western blotting, immunohistochemistry, and more. Alexa Fluor and Alexa Fluor Plus secondary antibodies offer advantages of brighter signal, multiplexing capabilities, and ease of use. Secondary antibodies are great for fixed cell imaging applications for subcellular protein targets that need a primary antibody to be identified.
Invitrogen secondary antibodies offer both high-activity horseradish peroxidase and alkaline phosphatase secondary antibody conjugates for immunohistochemistry, western blotting, ELISA, or other enzyme-driven applications. Also available are a wide variety of biotin and DSB biotin conjugates that allow for use of the same secondary antibody in multiple applications, simply by switching the streptavidin being used.
Regular instrument calibration and adjustment are required for high-precision imaging of fluorescent probes, particularly in multicolor applications such as confocal laser scanning microscopy that involve multiple exposures, repetitive scans, or three-dimensional sectioning. Invitrogen offers imaging dye standards, InSpeck intensity calibration beads, FocalCheck calibration beads and test slides, TetraSpeck microspheres, and FluoCells prepared slides.
Imaging dishes, plates, and coverglass
Maximize performance with Thermo Scientific Nunc Cell Culture Imaging Products. We offer a variety of plastics for cell imaging applications in fluorescence microscopy, phase contrast microscopy, confocal microscopy, live cell imaging, and fluorescence in situ hybridization (FISH).
Imaging media, mountants, and antifades
ProLong and SlowFade mounting medias are exceptional at maintaining bright fluorescent signals and reducing photobleaching for imaging cells or tissue. By using high quality mountant and antifade medias, coupled with fluorescent imaging dyes or antibodies, you can increase fluorophore photostability in both live-cell and fixed-cell samples for immediate imaging and for long-term storage.
Gibco FluoroBrite DMEM media is formulated so that background fluorescence is 90% lower than that emitted by standard phenol red–free DMEM media. It is designed to enhance the signal-to-noise ratio of fluorophores so that researchers can visualize weaker fluorescent signals due to hard-to-detect or low-expressing targets–all while maintaining cellular health and preventing photobleaching.
Balanced salt solutions can provide an environment that maintains the structural and physiological integrity of cells, critical for cell imaging workflows, and for achieving the highest quality and consistent results.
ReadyProbes Ready-to-Use Imaging Reagents
Invitrogen ReadyProbes reagents are ready-to-use imaging solutions designed to allow you to stain cells with a simple protocol that does not require any calculation, dilution, or pipetting. ReadyProbes dropper bottles have been formulated with some of the most popular imaging reagents including fluorescent secondary antibodies, cellular organelle labels, and cell health probes.
Fluorescent detection of subcellular organelles with selective stains, dyes, or specific antibodies is critical for imaging cells and tissue for any biological research application. Organelle stains can be used as counterstains to help identify the location of specific proteins and targets of interest within the cell, while imaging antibodies against proteins associated with specific organelles can lead to a better understanding of cellular function.