SulfoLink™ 偶联树脂
SulfoLink™ 偶联树脂
Thermo Scientific™

SulfoLink™ 偶联树脂

Thermo Scientific SulfoLink 偶联树脂是一种已经过碘乙酸基团活化的多孔交联 6% 微珠状琼脂糖，用于实现半胱氨酸多肽和其他巯基分子的共价固定。SulfoLink 偶联树脂的特点：•巯基 (-SH) 基团特异性—碘乙酸基团与巯基进行特异性反应，形成不可逆的硫醚键•了解更多信息
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货号数量
20404250 mL
2040110 mL
2040250 mL
货号 20404
价格（CNY)
43,556.00
Each
添加至购物车
数量:
250 mL
请求批量或定制报价
价格（CNY)
43,556.00
Each
添加至购物车
Thermo Scientific SulfoLink 偶联树脂是一种已经过碘乙酸基团活化的多孔交联 6% 微珠状琼脂糖，用于实现半胱氨酸多肽和其他巯基分子的共价固定。

SulfoLink 偶联树脂的特点：

•巯基 (-SH) 基团特异性—碘乙酸基团与巯基进行特异性反应，形成不可逆的硫醚键
• 快速—离心柱可提高方案速度；在 2 小时（肽）到 3.5 小时（蛋白）内制备和偶联样品
• 灵活的偶联条件—可根据偶联反应期间的蛋白或肽溶解度需求，使用 pH 值 7.5 到 9.0 的水缓冲液、有机溶剂（例如 20% DMSO）或变性剂（胍·HCl）
• 易于遵循的说明—简化了适用于样品制备、固定化和亲和纯化的方案
• 高容量—每 2 mL SulfoLink 耦合树脂柱可固定 1 至 2 mg 肽或 2 至 20 mg 蛋白

在用含还原半胱氨酸残基的肽或蛋白溶液孵育时，SulfoLink 树脂碘乙酸基团与暴露的巯基 (-SH) 发生特异性高效反应，形成永久性地将肽或蛋白连接到树脂的共价且不可逆硫醚键。产物是一种用于纯化各种抗体、抗原和其它感兴趣分子的定制亲和树脂。选择适用于肽或蛋白的两种便利的 SulfoLink 固定化试剂盒之一。每个试剂盒包含制备样品的所有试剂以及含有 2mL SulfoLink 树脂的五个柱，它们可通过重力流或离心操作形式用于进行高效偶联反应和多个周期的亲和纯化。还可提供用于各种肽或蛋白的单柱试剂盒试用装，也可以单独购买 SulfoLink 偶联树脂。

应用：
• 固定含末端胱氨酸残基的各种肽以纯化由通过马来酰亚胺偶联制备的肽免疫原生成的各种抗体
• 通过铰链区巯基，以定向方式固定各种抗体以确保抗原结合位点没有受到位阻，可进行抗原亲和纯化

相关产品
适用于肽的 SulfoLink™ 固定化试剂盒，2 mL
适用于蛋白的 SulfoLink™ 固定化试剂盒，2 mL
SulfoLink™ 固定化试剂盒试用装，2 mL
仅供科研使用。不可用于诊断程序。
规格
色谱柱类型亲和，琼脂糖树脂
产品规格瓶装
数量250 mL
固定相卤乙酰
足够用于1 mg 肽或 10 mg 抗体/mL 树脂
产品线SulfoLink™
类型树脂
Unit SizeEach

常见问题解答 (FAQ)

What is the binding capacity of SulfoLink Resin?

SulfoLink Resin can bind approximately 5 mg of reduced IgG per mL of support. For peptides, the capacity is approximately 1 mg of peptide per mL of gel. Capacity for other compounds will vary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When using the SulfoLink Coupling Resins and Kits, my peptide is not soluble in the coupling buffer. What can I do?

SulfoLink Immobilization is compatible with 3-4 M fresh urea or guanidine. Alternatively, dissolve the peptide in 100% DMSO. Add the peptide in DMSO to the coupling buffer so that the DMSO does not exceed 20% of the final solution.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Must I block the resin before each use when using the SulfoLink Coupling Resins and Kits?

No. The resin will no longer react with subsequent sulfhydryls after the ligand is attached and the remaining active sites are blocked.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How many purifications can I perform using the same SulfoLink Column?

The columns can be reused at least 10 times without loss of activity.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How do I perform affinity purifications with conjugated SulfoLink Affinity Columns?

Equilibrate the column with 3-5 bed-volumes of an appropriate binding buffer. Add 1 mL of sample for each 2 mL column (serum should be diluted at least 1:1 with binding buffer). Add an additional 200 µl of binding buffer to ensure that the entire sample has entered the gel bed. Cap the column bottom and top. Incubate the column for 1 hour. Wash away non-bound proteins with 5-7 bed-volumes of binding buffer or 1 M NaCl. Elute the bound sample by adding small fractions (0.5-1.0 ml) of elution buffer such as Thermo Scientific IgG Elution Buffer (Cat. No. 21004).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all SulfoLink™ 偶联树脂 FAQs