Pierce™ 考马斯 (Bradford) 蛋白检测试剂盒
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Pierce™ 考马斯 (Bradford) 蛋白检测试剂盒
Thermo Scientific™

Pierce™ 考马斯 (Bradford) 蛋白检测试剂盒

Pierce 考马斯 (Bradford) 蛋白检测试剂盒是一种即用型、稳定的传统 Bradford 检测试剂配方，用于测定相比于蛋白标准品的总蛋白浓度。试剂盒包括考马斯蛋白检测试剂和一系列白蛋白标准品安瓿。比较所有可用的 Bradford了解更多信息
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货号数量
23200950 mL
货号 23200
价格（CNY)
1,428.00
飞享价
Ends: 31-Dec-2025
1,947.00
共减 519.00 (27%)
Each
添加至购物车
数量:
950 mL
请求批量或定制报价
价格（CNY)
1,428.00
飞享价
Ends: 31-Dec-2025
1,947.00
共减 519.00 (27%)
Each
添加至购物车
Pierce 考马斯 (Bradford) 蛋白检测试剂盒是一种即用型、稳定的传统 Bradford 检测试剂配方，用于测定相比于蛋白标准品的总蛋白浓度。试剂盒包括考马斯蛋白检测试剂和一系列白蛋白标准品安瓿。

比较所有可用的 Bradford 检测›

Pierce 考马斯蛋白检测试剂盒是一种广泛采用的检测试剂的即用型配方，最初由 Bradford 在 1976 年描述。与蛋白溶液混合时，酸性考马斯染料试剂的颜色以与样品中存在的蛋白量成比例的方式从棕色变为蓝色。通过与通常制备作为牛血清白蛋白 (BSA) 或牛丙种球蛋白 (BGG) 的已知稀释系列的蛋白检测标准品的颜色响应进行比较，进行蛋白测定。简单程序适用于几乎任何体积标度，包括试管、比色皿和微孔板。蛋白检测与蛋白样品中遇到的大多数盐、溶剂、缓冲液、硫醇、还原性物质和金属螯合剂兼容。

考马斯蛋白检测试剂盒的特点包括：
•Bradford 试剂—经典 Bradford 检测试剂的稳定、即用型试剂盒
•比色—使用标准分光光度计或酶标仪在 595 nm
下测定•易于使用—单一试剂；无需制备工作试剂
•快速—几乎立即显色；添加、混合和读取结果
•检测范围—在 1 至 1500 μg/mL 检测蛋白浓度
•灵活—提供且适用于几种目标工作范围的微孔板和试管方案

考马斯 (Bradford) 测定试剂盒如何检测蛋白
基于考马斯染料 (Bradford) 的蛋白检测中的显色与蛋白中某种碱性氨基酸（主要是精氨酸、赖氨酸和组氨酸）的存在有关。范德华力和疏水作用也参与了蛋白质和染料的结合。结合到每个蛋白质分子上的考马斯染料的数目大致与蛋白所带正电荷的数量成正比。游离氨基酸、肽和低分子量蛋白质不会与考马斯染料试剂产生颜色。一般而言，肽或蛋白的质量必须至少为 3,000 道尔顿才能够使用该试剂检测到。

相关产品
Pierce 考马斯 Plus (Bradford) 检测试剂盒
Pierce 去污剂兼容 Bradford 检测试剂盒
安瓿破碎器
仅供科研使用。不可用于诊断程序。
规格
检测Bradford 检测
适用于（应用）基于溶液的检测、吸光度
适用于（设备）分光光度计、酶标仪
产品线Pierce™
产品类型蛋白定量检测
数量950 mL
特异性无靶标特异性
足够用于630 次试管检测或 3800 次微孔板检测
检测方法比色法
Unit SizeEach
内容与储存
足够用于：
  • 630 管检测或 3800 微孔板检测
  • Pierce 考马斯检测试剂，950 mL
  • 白蛋白标准品安瓿，2 mg/mL，10 x 1 mL
将考马斯蛋白检测试剂储存于 4°C 下。将未开封白蛋白标准品安瓿于室温下储存。

常见问题解答 (FAQ)

Can you provide the shelf-life for the Pierce Bradford Protein Assay Kit?

The Pierce Bradford Protein Assay Kit is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I purchase the Pierce Bradford Protein Assay Reagent from the Pierce Bradford Protein Assay Kit as a stand-alone product?

Sorry, the Pierce Bradford Protein Assay Reagent is only available as a component of the Pierce Bradford Protein Assay Kit.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

I assayed two protein samples, each containing a different mixture of proteins of same concentration and observed very different color responses in the assay. What is the cause?

Each of the commonly used total protein assay methods exhibits some degree of varying response toward different proteins. These differences relate to amino acid sequence, pI, structure and the presence of certain side chains or prosthetic groups that can dramatically alter the protein’s color response. Most protein assay methods use BSA or immunoglobulin (IgG) as the standard against which the concentration of protein in the sample is determined. However, if great accuracy is required, prepare the standard curve from a pure sample of the target protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My buffer or components of my buffer are not listed in the compatibility table for my protein assay. What should I do?

You can test the tolerance of the assay for your specific buffer formulation. For in-house generated compatibility information, substances were considered compatible at the indicated concentration in the Standard Test Tube Protocol (found in the manual for each protein assay) if the error in protein concentration estimation caused by the presence of the substance was less than or equal to 10%. The substances were tested using WR prepared immediately before each experiment. Blank-corrected 562nm absorbance measurements (for a 1000µg/mL BSA standard + substance) were compared to the net 562nm measurements of the same standard prepared in 0.9% saline.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

All the components of my sample buffer are at or below the indicated compatible concentration for my protein assay, but I am still seeing too much/too little color development. What could be the problem?

It is possible to have a substance additive affect such that even though a single component is present at a concentration below its listed compatibility, a sample buffer containing a combination of substances could interfere with the assay. You should take steps to eliminate or minimize the effects of the interfering substance(s) by diluting or removing the substance.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Pierce™ 考马斯 (Bradford) 蛋白检测试剂盒 FAQs