Apart from proteins, will the Photoreactive Amino Acids crosslink to other molecules?
Photoreactive amino acids are incorporated into proteins during synthesis; but upon UV activation, they can crosslink to other biomolecules within proximity.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
How stable are the Photoreactive Amino Acids in DMEM-LM?
Long-term stability of the photoreactive amino acids in media has not been determined but should be comparable to their natural analogs, if protected from light. For best results, store the photoreactive amino acids at -20°C as a dry compound. Just before use, add the photoreactive amino acids to the minimal volume of DMEM-LM supplemented with dialyzed serum.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
How long do I need to incubate the Photoreactive Amino Acids with my cells?
Incubate the photoreactive amino acids in DMEM-LM with cells for 24 hours. For proteins with high turnover, a minimum incubation of 8 hours is needed for detectable crosslinking levels. Incubation for longer than 24 hours might significantly affect cell growth or viability.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
My cells will not grow in DMEM, what other type of culture media can be used with the Photoreactive Amino Acids?
Some cells types can be adapted to grow in DMEM before using the DMEM-LM supplemented with the photoreactive amino acids. Currently we do not offer any other leucine- and methionine-depleted culture medium.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Can I use the Photoreactive Amino Acids to crosslink peptides?
Peptides can be synthesized using the N-termini-protected (Boc or Fmoc) photoreactive amino acid derivatives.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
