Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 制备试剂盒
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Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 制备试剂盒

Thermo Scientific Pierce 小鼠 IgG1 Fab 和 F(ab')2 制备试剂盒使用固定化无花果蛋白酶将小鼠 IgG1了解更多信息
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货号数量
449802.5 mL
货号 44980
价格(CNY)
11,139.00
Each
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数量:
2.5 mL
请求批量或定制报价
价格(CNY)
11,139.00
Each
添加至购物车
Thermo Scientific Pierce 小鼠 IgG1 Fab 和 F(ab')2 制备试剂盒使用固定化无花果蛋白酶将小鼠 IgG1 高效酶切为 Fab 或 F(ab')2 片段,具体取决于所选的反应条件。

小鼠 IgG1 Fab 和 F(ab')2 制备试剂盒的特点:

高容量—试剂盒可对 0.25 至 4 mg IgG 进行片段化。
完整—试剂盒包含制备和纯化抗体片段所需的所有试剂
无酶的酶切产品—固定化无花果蛋白酶(微珠状琼脂糖树脂)可用于控制酶切反应,并完全去除蛋白水解酶中的所得抗体片段
针对小鼠 IgG1 进行了优化—该试剂盒程序针对小鼠 IgG1 进行了优化;是对该 IgG 物种和亚类有效的方法(参见

相关产品
Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 微型制备试剂盒
Pierce™ F(ab')2 制备试剂盒
Pierce™ Fab 制备试剂盒
仅供科研使用。不可用于诊断程序。
规格
数量2.5 mL
产品线Pierce™
Unit SizeEach
内容与储存
足够用于:10 份抗体样品,每份含 0.25 至 4 mg IgG
• Pierce 无花果蛋白酶琼脂糖,2.5 mL
• 半胱氨酸-HCl,1 g
• 小鼠 IgG1 酶切缓冲液,120 mL
• NAb 蛋白 A Plus 离心柱,1 mL,1 根柱
• 蛋白 A 结合缓冲液,120 mL
• IgG 洗脱缓冲液,120 mL
• Zeba 脱盐离心柱,7K MWCO,2 mL,10 根柱
• 离心柱,0.8 mL,10 根柱
• 微量离心管,2 mL,30 个管

储存在 4–8°C 下。

常见问题解答 (FAQ)

Can I reuse the immobilized digestion enzymes in the Thermo Scientific IgG Fragmentation kit?

No. For best results, use the immobilized enzyme once and discard after use.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long does IgG digestion take using the Thermo Scientific IgG Fragmentation kit?

The digestion time is a function of the IgG concentration and source. For rabbit, mouse and human IgG, optimized digestion times are given in the instructions for each kit. Digestion times listed in the product instructions result in 80-90% digestion, although efficiency varies depending on IgG preparation and source.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

In the Thermo Scientific IgG Fragmentation kit, when digesting an IgG that does not bind to Protein A, how do I purify the Fab and F(abÁ)2 fragments?

If the IgG is a species or subclass that does not bind to Protein A, researchers can immobilize an antibody specific for the desired fragment. For example, to purify digested rat IgG2a, use the Aminolink Plus Immobilization Kit (Cat. No. 44894) to immobilize goat anti-rat Fc antibody. Our Tech Tip #34 and other web resources list IgG binding properties of Protein A. (Because Protein G and Protein A/G bind Fab fragments, they are not effective for this application.)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I purchase the immobilized enzymes (proteases) from the Thermo Scientific IgG Fragmentation kit separately?

Yes. The three immobilized enzymes are available in 5 mL package sizes as shown below:

• Immobilized Ficin, Cat. No. 44881
• Immobilized Papain, Cat. No. 20341
• Immobilized Pepsin, Cat. No. 20343

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many Thermo Scientific IgG Fragmentation kits are available and which enzymes are included?

There are six Thermo Scientific IgG fragmentation kits:

• Fab Preparation Kit (Papain), Cat. No. 44985
• Fab Micro Preparation Kit (Papain), Cat. No. 44685
• F(abÁ)2 Preparation Kit (Pepsin), Cat. No. 44988
• F(abÁ)2 Micro Preparation Kit, (Pepsin), Cat. No. 44688
• Mouse IgG1 Fab and F(abÁ)2 Preparation Kit (Ficin), Cat. No. 44980
• Mouse IgG1 Fab and F(abÁ)2 Micro Preparation Kit (Ficin), Cat. No. 44680

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.