Pierce™ F(ab')2 制备试剂盒
Pierce™ F(ab')2 制备试剂盒
Thermo Scientific™

Pierce™ F(ab')2 制备试剂盒

Thermo Scientific Pierce F(ab')2 制备试剂盒使用固定化胃蛋白酶选择性酶切完整的人和其他 IgG 抗体,以制成保留抗原结合活性的 F(ab')2 片段。F(ab')2了解更多信息
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货号数量
4498810份抗体样品,每份含0.25至 4 mg IgG
货号 44988
价格(CNY)
9,716.00
Each
添加至购物车
数量:
10份抗体样品,每份含0.25至 4 mg IgG
请求批量或定制报价
价格(CNY)
9,716.00
Each
添加至购物车
Thermo Scientific Pierce F(ab')2 制备试剂盒使用固定化胃蛋白酶选择性酶切完整的人和其他 IgG 抗体,以制成保留抗原结合活性的 F(ab')2 片段。

F(ab')2 微型制备试剂盒的特点:

无酶的酶切产品—固定化胃蛋白酶(微珠状琼脂糖树脂)可用于控制酶切反应,并完全去除蛋白水解酶中的所得抗体片段
适用于人和其他物种的 IgG—该试剂盒程序针对人、兔和小鼠 IgG 进行了优化。基于胃蛋白酶的酶切对其他物种和亚类的 IgG 有效,但纯化步骤要求抗体与蛋白 A 有效结合。(注:对于小鼠 IgG1,为获得最佳结果,请使用产品编号 44980。)
完整—试剂盒包括制备和纯化抗体片段所需的所有试剂
快速—离心形式可大大减少样品处理时间
灵活—包含用于多种物种和 IgG 亚类以及样品大小和浓度的方案。
高效—增强得率和样品纯度

这些 F(ab')2 制备试剂盒适用于人、兔、小鼠和其他物种和亚类的 IgG,小鼠 IgG1 除外。在便利的一次性离心柱中使用胃蛋白酶琼脂糖进行抗体酶切反应,可高效去除固定化蛋白酶并尽可能回收 IgG 片段。试剂盒中还包含 Thermo Scientific NAb 蛋白 A 离心柱和缓冲液,能够高效纯化所得片段。蛋白 A 可结合 Fc 片段和未酶切的 IgG,在流过物组分中回收纯 F(ab')2 片段。该试剂盒还包含 Thermo Scientific Zeba 脱盐离心柱,用于快速制备 IgG 样品,无需稀释且并不利用耗时的透析步骤。

该试剂盒使用胃蛋白酶(非特异性肽链内切酶)对整个 IgG 的 Fc 部分进行酶促酶切,得到称为 F(ab')2 的片段。该片段由一对通过两个二硫键连接的 Fab' 单元构成。由于胃蛋白酶以固定化形式的微珠状琼脂糖树脂提供,可通过去除 IgG 溶液中的树脂轻松停止酶切反应;结果是得到无酶的酶切产品。

相关产品
Pierce™ F(ab')2 微型制备试剂盒
Pierce™ Fab 微型制备试剂盒
Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 微型制备试剂盒
仅供科研使用。不可用于诊断程序。
规格
描述Pierce F(ab')2 制备试剂盒
数量10份抗体样品,每份含0.25至 4 mg IgG
产品线Pierce™
Unit SizeEach
内容与储存
足够用于:10 份抗体样品,每份含 0.25 至 4 mg IgG
• Pierce 胃蛋白酶琼脂糖,1.25 mL
• F(ab')2 酶切缓冲液,120 mL
• NAb 蛋白 A Plus 离心柱,1 mL,1 根柱
• PBS 包(每个可制备 500 mL),2 包
• IgG 洗脱缓冲液,120 mL
• Zeba 脱盐离心柱,7K MWCO,2 mL,10 根柱
• 离心柱,0.8 mL,10 根柱
• 微量离心管,2 mL,30 个管

储存在 4–8°C 下

常见问题解答 (FAQ)

Can I reuse the immobilized digestion enzymes in the Thermo Scientific IgG Fragmentation kit?

No. For best results, use the immobilized enzyme once and discard after use.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long does IgG digestion take using the Thermo Scientific IgG Fragmentation kit?

The digestion time is a function of the IgG concentration and source. For rabbit, mouse and human IgG, optimized digestion times are given in the instructions for each kit. Digestion times listed in the product instructions result in 80-90% digestion, although efficiency varies depending on IgG preparation and source.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

In the Thermo Scientific IgG Fragmentation kit, when digesting an IgG that does not bind to Protein A, how do I purify the Fab and F(abÁ)2 fragments?

If the IgG is a species or subclass that does not bind to Protein A, researchers can immobilize an antibody specific for the desired fragment. For example, to purify digested rat IgG2a, use the Aminolink Plus Immobilization Kit (Cat. No. 44894) to immobilize goat anti-rat Fc antibody. Our Tech Tip #34 and other web resources list IgG binding properties of Protein A. (Because Protein G and Protein A/G bind Fab fragments, they are not effective for this application.)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I purchase the immobilized enzymes (proteases) from the Thermo Scientific IgG Fragmentation kit separately?

Yes. The three immobilized enzymes are available in 5 mL package sizes as shown below:

• Immobilized Ficin, Cat. No. 44881
• Immobilized Papain, Cat. No. 20341
• Immobilized Pepsin, Cat. No. 20343

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many Thermo Scientific IgG Fragmentation kits are available and which enzymes are included?

There are six Thermo Scientific IgG fragmentation kits:

• Fab Preparation Kit (Papain), Cat. No. 44985
• Fab Micro Preparation Kit (Papain), Cat. No. 44685
• F(abÁ)2 Preparation Kit (Pepsin), Cat. No. 44988
• F(abÁ)2 Micro Preparation Kit, (Pepsin), Cat. No. 44688
• Mouse IgG1 Fab and F(abÁ)2 Preparation Kit (Ficin), Cat. No. 44980
• Mouse IgG1 Fab and F(abÁ)2 Micro Preparation Kit (Ficin), Cat. No. 44680

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.