Viral Production Cells

Gibco Viral Production Cells are a derivative of the HEK 293F cell line and are a core component of the LV-MAX Lentiviral Production System (Cat. No. A35684). Viral Production Cells have been adapted to a special chemically-defined, serum-free and protein-free LV-MAX Production Medium (A3583401). They are maintained in suspension culture and will grow to high density in LV-MAX Production Medium. Viral Production Cells are highly transfectable by LV-MAX Transfection reagent (A35348) and generate superior lentiviral production compared to polyethyleneimine (PEI)-based lentiviral production methods.

• Suspension culture cells facilitate high-throughput screening or scale-up
• Higher viral production levels than other suspension HEK 293 cells
• Rapid cell growth and high culture viabilities

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 10⁷ viable cells/mL in LV-MAX Production Medium and 10% DMSO. The cells should be thawed directly into LV-MAX Production Medium.

The cells are typically grown in flasks on a shaker platform in a humidified, CO₂ cell culture incubator. However, cultures are scalable in volume from 96-deep well blocks to 2-L flasks and bioreactors. Lentiviral vector harvest is typically performed at two days post-transfection.