LV-MAX™ 慢病毒生产系统
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LV-MAX™ 慢病毒生产系统
Gibco™

LV-MAX™ 慢病毒生产系统

Gibco LV-MAX 慢病毒生产系统是较早经过优化的系统,提供可扩展、高产量的慢病毒载体生产平台。其基于 HEK 293 来源的病毒生产细胞的悬浮高密度培养物,适应化学成分明确的特殊无血清和无蛋白 LV-MAX 生产培养基了解更多信息
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货号数量
A356841 个试剂盒
货号 A35684
价格(CNY)
44,687.00
Each
添加至购物车
数量:
1 个试剂盒
价格(CNY)
44,687.00
Each
添加至购物车
Gibco LV-MAX 慢病毒生产系统是较早经过优化的系统,提供可扩展、高产量的慢病毒载体生产平台。其基于 HEK 293 来源的病毒生产细胞的悬浮高密度培养物,适应化学成分明确的特殊无血清和无蛋白 LV-MAX 生产培养基。通过我们先进的脂质体纳米微粒转染试剂结合新型的慢病毒特异性增强剂和补充剂,调控实现较优的病毒滴度产量。相较于传统的聚乙烯亚胺 (PEI),所有组分协同作用,可在无血清条件下产生优良的功能性慢病毒微粒。

LV-MAX 慢病毒生产系统提供:
•高滴度—大于 1 x 108 TU/mL(未浓缩的 LVVs-GFP)
• 可扩展的悬浮系统—从 96 深孔板到 2-L 生物反应器
• 无血清—消除了动物源性组分和相关的风险
• 简单稳健的培养和转染方案—适合当前的瞬时生产工作流程

LV-MAX 慢病毒生产系统包含的组分包括:两瓶冷冻病毒生产细胞,一升 LV-MAX 生产培养基和一个足以生产 0.3 L 慢病毒的 LV-MAX 转染试剂盒。LV-MAX 慢病毒生产系统的所有组分也可单独购买。该试剂盒中未包括但还需要的是 Gibco Opti-MEM I 减血清培养基(货31985062)封闭膜。

仅供科研使用。不可用于诊断程序。
规格
细胞系病毒生产细胞系
输送类型慢病毒、转染
表达系统瞬时
产品线LV-MAX™
产品类型慢病毒生产系统
数量1 个试剂盒
运输条件经批准可在室温下或者湿冰或干冰上运输
培养类型悬浮液细胞培养
形式液体
Unit SizeEach
内容与储存
• 1 L LV-MAX 生产培养基。在 2–8°C 下避光储存。
• 0.3 L LV-MAX 转染试剂盒。在 2–8°C 下避光储存。
•2 瓶病毒生产细胞。请储存于液氮中直至使用。请勿将细胞储存于 -80°C 条件下。

常见问题解答 (FAQ)

What are the major differences between LV-MAX and CTS LV- MAX products?

The LV-MAX and CTS LV- MAX products have equivalent performance. In contrast to the research use only LV-MAX products, CTS LV-MAX products have an additional intended use for cell and gene therapy applications, and are complemented with a more robust regulatory support package to enable clinical therapeutic applications. This means that to the best of our ability, we keep our CTS product specifications and release requirements up-to-date in accordance with the latest regulatory guidance specific for this intended use.

What is the intended use claim for your LV-MAX and CTS LV-MAX products?

Our LV-MAX products are intended for research use only while our CTS LV- MAX products are intended for research use or manufacture of cell, gene or tissue based products. Please refer to the individual products for further details on the intended use claim.

Do you offer a lentivirus production service?

Yes. Please provide your details here (https://www.thermofisher.com/us/en/home/global/forms/life-science/lentiviral-vector-production-get-info.html) and mention your interest in our lentivirus production services. A member of our services team will contact you shortly.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

With the LV-MAX Production System, are the lentiviral particles stable in packaging media?

Lentiviral particles packaged in our LV-MAX Production Medium will be stable at 4 degrees C for 24 hours and will then begin to lose activity. For long-term storage, we recommend storing at –80 degrees C.

With the LV-MAX Production System, can I use cell lines besides HT1080 for titering my lentivirus?

We strongly recommend the human fibrosarcoma line HT1080 (ATCC, Cat. No. CCL-121) as the “gold standard” for reproducibly titering lentivirus. However, you may wish to use the same mammalian cell line to titer your lentiviral stocks as you will use to perform your expression studies (e.g., if you are performing expression studies in a dividing cell line or a non-primary cell line). If you have more than one lentiviral construct, we recommend that you titer all of the lentiviral constructs using the same mammalian cell line.