引用和文献 (13)

Thermo Scientific™

EZ-Link™ NHS-PEG4-生物素

Name: EZ-Link™ NHS-PEG4-生物素
SKU: A39259
Price: 2158.00 CNY

Thermo Scientific EZ-Link NHS-PEG4-生物素是一种聚乙二醇化水溶性试剂，可对抗体、蛋白和其他含伯胺的大分子进行简单高效的生物素标记。EZ-Link NHS-PEG4-生物素的特点：•蛋白标记—使用链霉素亲和素探针或树脂对抗体或其他蛋白进行生物素化，用于检测或纯化•胺反应性—与伯胺 (-NH2) 发生反应，例如赖氨酸 (K)了解更多信息

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货号	数量
A39259	10 x 2 mg
21330	25 mg
21362	50 mg
21363	1 g

4 选项

货号 A39259

价格（CNY)

2,158.00

飞享价

Ends: 31-Dec-2026

3,127.00

共减 969.00 (31%)

Each

数量:

10 x 2 mg

价格（CNY)

2,158.00

飞享价

Ends: 31-Dec-2026

3,127.00

共减 969.00 (31%)

Each

Thermo Scientific EZ-Link NHS-PEG4-生物素是一种聚乙二醇化水溶性试剂，可对抗体、蛋白和其他含伯胺的大分子进行简单高效的生物素标记。

EZ-Link NHS-PEG4-生物素的特点：

•蛋白标记—使用链霉素亲和素探针或树脂对抗体或其他蛋白进行生物素化，用于检测或纯化
•胺反应性—与伯胺 (-NH2) 发生反应，例如赖氨酸 (K) 的侧链或多肽的氨基端
•聚乙二醇化–间隔臂含有一个亲水性 4 单位聚乙二醇 (PEG) 基团
•增强溶解度–聚乙二醇化使生物素化分子具有水溶性，有助于防止储存在溶液中的生物素化抗体的聚集
•不可逆—形成永久性酰胺键；间隔臂不能被裂解
•长距离–间隔臂（添加至靶标的总长度）为 29 埃；这可以减少与亲和素分子结合的空间位阻

NHS-PEG4 生物素是长 (29.0Å) 聚乙二醇化、水溶性、NHS 酯生物素化试剂，用于标记胺并尽可能增加抗体和其他蛋白的溶解度。N-羟基琥珀酰亚胺 (NHS) 基团与赖氨酸和 N 端氨基基团发生特异性高效反应，形成稳定的酰胺键。亲水性聚乙二醇 (PEG) 间隔臂可赋予水溶性，将其转移至生物素化分子，从而减少储存在溶液中的标记蛋白的聚集。PEG 间隔臂还为该试剂提供了长而灵活的连接，以尽可能减少与亲和素分子结合时的空间位阻。

我们生产生物素试剂，以确保尽可能使预期研究应用的总体产品完整性、一致性和性能达到较高水平。

生物素的 N-羟基磺基琥珀酰亚胺 (NHS) 酯是生物素化试剂广泛采用的类型。NHS-活化生物素在碱性缓冲液中与伯胺基 (-NH2) 高效反应，形成稳定的酰胺键。蛋白（如抗体）通常有几种可作为标记靶标的伯胺，包括赖氨酸 (K) 残基的侧链和每个多肽的 N-末端。

生物素各种 NHS 酯试剂在长度、溶解度、细胞通透性和可切割性方面有所不同。非磺化 NHS-生物素具有细胞渗透性，但必须在有机溶剂（如 DMSO 或 DMF）中溶解。Sulfo-NHS 生物素（和带有聚乙二醇化间隔的生物素）可以直接溶解在水中，但无膜渗透性。含二硫键的各种类型可使用还原剂进行裂解，从而使生物素基团与标记蛋白分离。

相关产品
EZ-Link™ NHS-PEG4 生物素化试剂盒
EZ-Link™微型 NHS-PEG4-生物素化试剂盒

仅供科研使用。不可用于诊断程序。

规格

细胞渗透性细胞不可透过性

标记方法化学标记

产品线EZ-Link

产品类型NHS-PEG4-生物素

数量10 x 2 mg

反应一部分NHS 酯

运输条件湿冰

化学反应性胺

标签或染料生物素

溶解度DMF（二甲基甲酰胺）, DMSO（二甲亚砜）, 水

间隔子聚乙二醇化，29.0 Å

Unit SizeEach

内容与储存

保存于 -20°C。

常见问题解答 (FAQ)

Can you provide the shelf-life for the EZ-Link NHS-SS-PEG4-Biotin?

The EZ-Link NHS-SS-PEG4-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (-20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

引用和文献 (13)

引用和文献

Abstract

Pre-fusion RSV F strongly boosts pre-fusion specific neutralizing responses in cattle pre-exposed to bovine RSV.

Authors:Steff AM, Monroe J, Friedrich K, Chandramouli S, Nguyen TL, Tian S, Vandepaer S, Toussaint JF, Carfi A

Journal:Nat Commun

PubMed ID:29057917

'Human respiratory syncytial virus (hRSV) is responsible for serious lower respiratory tract disease in infants and in older adults, and remains an important vaccine need. RSV fusion (F) glycoprotein is a key target for neutralizing antibodies. RSV F stabilized in its pre-fusion conformation (DS-Cav1 F) induces high neutralizing antibody titers ... More

Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection.

Authors:Achtsnicht S, Neuendorf C, Faßbender T, Nölke G, Offenhäusser A, Krause HJ, Schröper F

Journal:PLoS One

PubMed ID:31276546

'Cholera is a life-threatening disease caused by the cholera toxin (CT) as produced by some Vibrio cholerae serogroups. In this research we present a method which directly detects the toxin''s B subunit (CTB) in drinking water. For this purpose we performed a magnetic sandwich immunoassay inside a 3D immunofiltration column. ... More

Iterative Design and Optimization of Initially Inactive Proteolysis Targeting Chimeras (PROTACs) Identify VZ185 as a Potent, Fast, and Selective von Hippel-Lindau (VHL) Based Dual Degrader Probe of BRD9 and BRD7.

Authors:Zoppi V, Hughes SJ, Maniaci C, Testa A, Gmaschitz T, Wieshofer C, Koegl M, Riching KM, Daniels DL, Spallarossa A, Ciulli A

Journal:J Med Chem

PubMed ID:30540463

'Developing PROTACs to redirect the ubiquitination activity of E3 ligases and potently degrade a target protein within cells can be a lengthy and unpredictable process, and it remains unclear whether any combination of E3 and target might be productive for degradation. We describe a probe-quality degrader for a ligase-target pair ... More

Dynamics of the Eukaryotic Replicative Helicase at Lagging-Strand Protein Barriers Support the Steric Exclusion Model.

Authors:Kose HB, Larsen NB, Duxin JP, Yardimci H

Journal:Cell Rep

PubMed ID:30784593

'Progression of DNA replication depends on the ability of the replisome complex to overcome nucleoprotein barriers. During eukaryotic replication, the CMG helicase translocates along the leading-strand template and unwinds the DNA double helix. While proteins bound to the leading-strand template efficiently block the helicase, the impact of lagging-strand protein obstacles ... More

Pan-Filovirus Serum Neutralizing Antibodies in a Subset of Congolese Ebolavirus Infection Survivors.

Authors:Bramble MS, Hoff N, Gilchuk P, Mukadi P, Lu K, Doshi RH, Steffen I, Nicholson BP, Lipson A, Vashist N, Sinai C, Spencer D, Olinger G, Wemakoy EO, Illunga BK, Pettitt J, Logue J, Marchand J, Varughese J, Bennett RS, Jahrling P, Cavet G, Serafini T, Ollmann Saphire E, Vilain E, Muyembe-Tamfum JJ, Hensely LE, Simmons G, Crowe JE, Rimoin AW

Journal:J Infect Dis

PubMed ID:30107445

One year after a Zaire ebolavirus (EBOV) outbreak occurred in the Boende Health Zone of the Democratic Republic of the Congo during 2014, we sought to determine the breadth of immune response against diverse filoviruses including EBOV, Bundibugyo (BDBV), Sudan (SUDV), and Marburg (MARV) viruses. After assessing the 15 survivors, ... More

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