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Trypsin and Lys-C are present in a 1:1 ratio in Trypsin/Lys-C Protease Mix, MS Grade (Cat. No. A41007). The mix contains 10 µg of each protease.

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Trypsin (Cat. Nos. 90057, 90058) or Trypsin/LysC mix (Cat. Nos. A40007, A40009, A41007) are most commonly used for proteomic applications in order to ensure reproducibility and complete digestion. Other commonly used enzymes for purified protein characterization and unique applications include Chymotrypsin Protease, MS Grade (Cat. No. 90056), Immobilized Pepsin (Cat. No. 20343), LysN Protease, MS Grade (Cat. No. 90300), Asp-N Protease, MS Grade (Cat. No. 90053), and Glu-C Protease, MS Grade (Cat. No. 90054).

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Yes, the MS-Grade trypsin protease in this mix is derived from porcine pancreatic extracts and has been Tosyl phenylalanyl chloromethyl ketone (TPCK)-treated to eliminate chymotryptic activity, and methylated to improve stability during digestion.

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Trypsin cuts at the carboxyl end of lysine and arginine residues, and Lys-C protease cuts at the carboxyl end of just lysine residues with Lys-C being more efficient than trypsin. Although trypsin is routinely used for protein digestion, this protease alone is not sufficient to fully digest proteins at the carboxyl-end of lysine and arginine residues. Therefore, Lys-C is combined with trypsin to sequentially digest proteins with fewer missed cleavage points at lysines.

The added benefit of having trypsin and Lys-C together is that unlike trypsin, Lys-C can cleave lysines followed by prolines.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.