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多能干细胞 (PSC) 基因编辑演示试剂盒
The Pluripotent Stem Cell (PSC) Gene Editing Demo Kit offers the critical reagents for successfully generating and detecting knock-outs and了解更多信息
| 货号 | 数量 |
|---|---|
| A43203 | 1 个试剂盒 |
货号 A43203
价格（CNY)
-
数量:
1 个试剂盒
The Pluripotent Stem Cell (PSC) Gene Editing Demo Kit offers the critical reagents for successfully generating and detecting knock-outs and point mutations in your cell line of interest.
• Reduce effort and cycle times
• Generate pools with high editing efficiency (up to 80% KO and 40% HDR)
• Quantitate editing efficiency
• Isolate clonal lines easily
What you get:
• StemFlex Medium (500 mL), comprised of:
--StemFlex Basal Medium (450 mL)
--StemFlex Supplement (50 mL)
• rhLaminin-521 (0.1 mg)
• RevitaCell Supplement (5 mL)
• TrueCut Cas9 Protein v2 (10 μg)
• TrueGuide sgRNA Positive Control, HPRT1
• GeneArt Genomic Cleavage Detection Kit
• Manual/protocol (electronic)
• Neon demo upon request
Efficient genome editing
TrueCut Cas9 protein v2 features include:
• High DNA cleavage and homology driven repair
• Complex with gRNAs into RNPs
• Deliver RNPs with Neon system or Lipofectamine Stem reagent
Superior single-cell survival
The StemFlex/Laminin-521 system with RevitaCell supplement enhances single-cell survival afterelectroporation and single cell sorting. Improved cell survival after electroporation reduces timelines, and the ability to single-cell sort hiPSCs reduces clonal isolation effort and timelines dramatically.
Rapid detection
The GeneArt Genomic Cleavage Detection Kit is a fast, PCR-based method to measure how well your genome editing tool forms insertion and deletion (indel) mutations. Directly PCR amplify your pool of edited cells with primers specific to your targeted region, run the cleavage assay, and determine efficiency, all in an afternoon.
• Reduce effort and cycle times
• Generate pools with high editing efficiency (up to 80% KO and 40% HDR)
• Quantitate editing efficiency
• Isolate clonal lines easily
What you get:
• StemFlex Medium (500 mL), comprised of:
--StemFlex Basal Medium (450 mL)
--StemFlex Supplement (50 mL)
• rhLaminin-521 (0.1 mg)
• RevitaCell Supplement (5 mL)
• TrueCut Cas9 Protein v2 (10 μg)
• TrueGuide sgRNA Positive Control, HPRT1
• GeneArt Genomic Cleavage Detection Kit
• Manual/protocol (electronic)
• Neon demo upon request
Efficient genome editing
TrueCut Cas9 protein v2 features include:
• High DNA cleavage and homology driven repair
• Complex with gRNAs into RNPs
• Deliver RNPs with Neon system or Lipofectamine Stem reagent
Superior single-cell survival
The StemFlex/Laminin-521 system with RevitaCell supplement enhances single-cell survival afterelectroporation and single cell sorting. Improved cell survival after electroporation reduces timelines, and the ability to single-cell sort hiPSCs reduces clonal isolation effort and timelines dramatically.
Rapid detection
The GeneArt Genomic Cleavage Detection Kit is a fast, PCR-based method to measure how well your genome editing tool forms insertion and deletion (indel) mutations. Directly PCR amplify your pool of edited cells with primers specific to your targeted region, run the cleavage assay, and determine efficiency, all in an afternoon.
仅供科研使用。不可用于诊断程序。
规格
细胞类型胚胎干细胞 (ESC)、诱导多能干细胞 (iPSC)
产品类型Pluripotent Stem Cell (PSC) Gene Editing Demo Kit
数量1 个试剂盒
Unit SizeEach
内容与储存
• StemFlex 培养基 (500 mL)，包括：
--StemFlex 基础培养基 (450 mL, 2–8°C)
--StemFlex 补充剂（50 mL，-5 至 -30°C）
• rhLaminin-521（0.1 mg，-5 至 -30°C）
• RevitaCell 补充剂（5 mL，-5 至 -30°C）
• TrueCut Cas9 蛋白 v2（10 µg，-5 至 -30°C）
• TrueGuide 阳性对照品指导 sgRNA，HPRT1（3 nmol；在室温下储存干燥的 RNA，在 -5 至 -30°C 下储存重悬的 RNA；在无核酸酶水或 TE 中重悬）
• 基因组 DNA 切割检测试剂盒（1 个试剂盒，-5 至 -30°C）
--StemFlex 基础培养基 (450 mL, 2–8°C)
--StemFlex 补充剂（50 mL，-5 至 -30°C）
• rhLaminin-521（0.1 mg，-5 至 -30°C）
• RevitaCell 补充剂（5 mL，-5 至 -30°C）
• TrueCut Cas9 蛋白 v2（10 µg，-5 至 -30°C）
• TrueGuide 阳性对照品指导 sgRNA，HPRT1（3 nmol；在室温下储存干燥的 RNA，在 -5 至 -30°C 下储存重悬的 RNA；在无核酸酶水或 TE 中重悬）
• 基因组 DNA 切割检测试剂盒（1 个试剂盒，-5 至 -30°C）