What is the binding capacity of the AminoLink Coupling Resin or aminoLink Plus Resin?
AminoLink Coupling Resin can bind approximately 10 mg of IgG/mL of resin bed, while AminoLink Plus Resin can bind up to 15-20 mg of IgG/mL of resin bed. The upper limit for binding peptides is approximately 1 mg/mL of resin for both support types.
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What are the differences between the two supports for the AminoLink Plus Resin?
The AminoLink Plus Resin uses a type of agarose that has better flow properties, which may shorten the time needed for drip-column methods. It is also activated at a higher level, allowing greater ligand to be immobilized.
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My ligand is not soluble in the coupling buffer when using the AminoLink Coupling Resins or Kits. What can I do?
Coupling reactions with AminoLink Resins are compatible with 3-4 M fresh urea or guanidine. (However, old solutions of urea may contain ammonia, which will interfere with ligand immobilization.) Alternatively, dissolve the peptide in 100% DMSO. Add the peptide in DMSO to coupling buffer so that the DMSO does not exceed 20% of the final solution.
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How many purifications can be performed using the same AminoLink or AminoLink Plus Column?
Columns can be reused at least 10 times without loss of activity.
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How long will the coupling reaction take when using the AminoLink Resins and Kits?
The immobilizatoin reaction takes six hours; the entire procedure may require seven to eight hours. This time can be decreased if the high pH protocol for AminoLink Plus Resin is used.
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