AminoLink™ Plus 偶联树脂
AminoLink™ Plus 偶联树脂
Thermo Scientific™

AminoLink™ Plus 偶联树脂

Thermo Scientific AminoLink Plus 偶联树脂使用醛活化琼脂糖微珠通过伯胺实现抗体(蛋白)高得率共价偶联,以制备亲和纯化柱。AminoLink Plus 偶联树脂的特点:•AminoLink了解更多信息
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货号数量
2050550 mL
2050110 mL
货号 20505
价格(CNY)
8,108.00
Each
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数量:
50 mL
请求批量或定制报价
价格(CNY)
8,108.00
Each
添加至购物车
Thermo Scientific AminoLink Plus 偶联树脂使用醛活化琼脂糖微珠通过伯胺实现抗体(蛋白)高得率共价偶联,以制备亲和纯化柱。

AminoLink Plus 偶联树脂的特点:

AminoLink Plus 偶联树脂 — 醛活化交联 4% 微珠状琼脂糖
适用于抗体和其他蛋白 — 通过伯胺 (-NH2) 固定分子
灵活的偶联条件 — 可在多种 pH 值 (4-10) 和缓冲液(PBS 或者含或不含有机溶剂的其他非胺缓冲液)条件下实现高效 (>85%) 偶联;提供常规(PBS,pH 值为7.2)和增强型(柠檬酸盐-碳酸盐,pH 值为10)偶联方案
稳定、永久固定 — 偶联反应在树脂与配体之间产生稳定、防渗漏的仲胺键
优于 CNBr 活化琼脂糖固定作用 — 化学键更为稳定且不带电荷,因而亲和纯化程序中的非特异性结合更少
通用且可重复使用 — 成品亲和树脂可适应柱和批次亲和技术,并根据蛋白结合相互作用为常规应用提供可重复使用的树脂

AminoLink Plus 树脂是经过改良含醛基的微珠状琼脂糖载体。抗体、其他蛋白或具有伯胺的任何分子可以通过优化的还原胺化形式与树脂相结合。一旦固定了一个抗体或其他配体,就可将制备好的亲和树脂用于各种涉及批或离心柱色谱分析的亲和纯化方法。树脂和连结在亲和色谱分析中通常使用的结合和洗脱条件下保持稳定,使得制备好的树脂可用于至少 10 次亲和纯化。

规格:
产品详情:
AminoLink Plus 偶联反应涉及醛(支持物上)与胺(配体上)之间自发形成席夫碱键,通过使用温和还原剂(氰基硼氢化钠)进行孵育,以实现后续稳定。整个偶联反应(称为还原胺化)发生在简单非胺缓冲液(如 PBS)中,在 4 至 6 小时内完成。与抗体和典型蛋白的偶联效率通常高于 85%,每毫升琼脂糖树脂可固定 1 至 20 mg 蛋白。

使用 AminoLink Plus 偶联树脂的偶联效率超过使用溴化氰 (CNBr) 活化支持物获得的偶联效率。此外,AminoLink Plus Chemistry 与含胺配体形成一个键,比 CNBr 方法更稳定且不带电荷。在亲和纯化过程中使用时,这些特性可提供更强的防渗漏固定作用和更低的非特异性结合。

相关产品
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AminoLink™ Plus 微固定试剂盒,0.1 mL
仅供科研使用。不可用于诊断程序。
规格
色谱柱类型亲和,琼脂糖树脂
产品规格瓶装
配方醛活化交联4%珠状琼脂糖,在水中与叠氮钠混合成浆状
数量50 mL
固定相醛类
靶标通过伯胺固定抗体、蛋白
产品线AminoLink™
类型树脂
Unit SizeEach

常见问题解答 (FAQ)

What is the binding capacity of the AminoLink Coupling Resin or aminoLink Plus Resin?

AminoLink Coupling Resin can bind approximately 10 mg of IgG/mL of resin bed, while AminoLink Plus Resin can bind up to 15-20 mg of IgG/mL of resin bed. The upper limit for binding peptides is approximately 1 mg/mL of resin for both support types.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the differences between the two supports for the AminoLink Plus Resin?

The AminoLink Plus Resin uses a type of agarose that has better flow properties, which may shorten the time needed for drip-column methods. It is also activated at a higher level, allowing greater ligand to be immobilized.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

My ligand is not soluble in the coupling buffer when using the AminoLink Coupling Resins or Kits. What can I do?

Coupling reactions with AminoLink Resins are compatible with 3-4 M fresh urea or guanidine. (However, old solutions of urea may contain ammonia, which will interfere with ligand immobilization.) Alternatively, dissolve the peptide in 100% DMSO. Add the peptide in DMSO to coupling buffer so that the DMSO does not exceed 20% of the final solution.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How many purifications can be performed using the same AminoLink or AminoLink Plus Column?

Columns can be reused at least 10 times without loss of activity.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How long will the coupling reaction take when using the AminoLink Resins and Kits?

The immobilizatoin reaction takes six hours; the entire procedure may require seven to eight hours. This time can be decreased if the high pH protocol for AminoLink Plus Resin is used.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.