LV-MAX™ 转染试剂盒
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LV-MAX™ 转染试剂盒
Gibco™

LV-MAX™ 转染试剂盒

Gibco LV-MAX 转染试剂盒是 LV-MAX 慢病毒生产系统的关键组成部分。用于瞬时转染 Gibco 病毒生产细胞(高密度悬浮培养驯化的人胚胎肾细胞 (HEK)了解更多信息
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货号数量
A353481 L
货号 A35348
价格(CNY)
17,183.00
Online Exclusive
Ends: 31-Dec-2025
24,010.00
共减 6,827.00 (28%)
Each
添加至购物车
数量:
1 L
价格(CNY)
17,183.00
Online Exclusive
Ends: 31-Dec-2025
24,010.00
共减 6,827.00 (28%)
Each
添加至购物车
Gibco LV-MAX 转染试剂盒是 LV-MAX 慢病毒生产系统的关键组成部分。用于瞬时转染 Gibco 病毒生产细胞(高密度悬浮培养驯化的人胚胎肾细胞 (HEK) 293衍生细胞株)。该系统包含高效的阳离子脂质转染试剂,慢病毒生产补充剂和慢病毒增强剂,它们可共同作用,在 LV-MAX 生产培养基中,培养的病毒生产细胞可生产最高较高水平的慢病毒颗粒。

•专为高密度悬浮细胞的转染而设计,可最大程度提高慢病毒生产
•可实现高密度瞬时转染培养物的持续、高水平生产,达到高达 1 x 108 TU/mL(未浓缩 LVV-GFP)的滴度
•可重现和可扩展的转染结果,让用户对他们的结果更有信心
•轻松、稳健的培养和转染方案—适用于任何瞬时生产工作流程。试剂盒包含优化慢病毒生产所需的三个关键组分。此类产品仅在试剂盒中提供,不单独出售。
•LV-MAX 转染试剂—高效的阳离子脂质转染试剂,专门用于将多种质粒共转染到高密度病毒生产细胞培养物中,具有高转染效率和低毒性
•LV-MAX 补充剂—优化的、化学成分明确的、无血清、无蛋白、非动物源性配方,可抑制转染过程中的细胞生长、提高慢病毒载体产量,而不会影响细胞活力或干扰慢病毒生产过程
•LV-MAX 增强剂—专利的非动物源性配方,用于提高细胞慢病毒载体产量

仅供科研使用。不可用于诊断程序。
规格
细胞系已建立的细胞系
分类非动物源性、化学成分明确、无蛋白、无血清
适用于(应用)转染
产品线LV-MAX™
产品类型转染试剂盒
数量1 L
血清兼容性
运输条件湿冰
细胞类型293 衍生细胞
样品类型质粒 DNA
转染技术脂质转染
Unit SizeEach
内容与储存
•50 mL LV-MAX 补充剂
•6 mL LV-MAX 转染试剂
•40 mL LV-MAX 增强剂

足以进行 1 L LV 生产

避光储存在 2-8°C 下。

常见问题解答 (FAQ)

What are the major differences between LV-MAX and CTS LV- MAX products?

The LV-MAX and CTS LV- MAX products have equivalent performance. In contrast to the research use only LV-MAX products, CTS LV-MAX products have an additional intended use for cell and gene therapy applications, and are complemented with a more robust regulatory support package to enable clinical therapeutic applications. This means that to the best of our ability, we keep our CTS product specifications and release requirements up-to-date in accordance with the latest regulatory guidance specific for this intended use.

What is the intended use claim for your LV-MAX and CTS LV-MAX products?

Our LV-MAX products are intended for research use only while our CTS LV- MAX products are intended for research use or manufacture of cell, gene or tissue based products. Please refer to the individual products for further details on the intended use claim.

Do you offer a lentivirus production service?

Yes. Please provide your details here (https://www.thermofisher.com/us/en/home/global/forms/life-science/lentiviral-vector-production-get-info.html) and mention your interest in our lentivirus production services. A member of our services team will contact you shortly.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

With the LV-MAX Production System, are the lentiviral particles stable in packaging media?

Lentiviral particles packaged in our LV-MAX Production Medium will be stable at 4 degrees C for 24 hours and will then begin to lose activity. For long-term storage, we recommend storing at –80 degrees C.

With the LV-MAX Production System, can I use cell lines besides HT1080 for titering my lentivirus?

We strongly recommend the human fibrosarcoma line HT1080 (ATCC, Cat. No. CCL-121) as the “gold standard” for reproducibly titering lentivirus. However, you may wish to use the same mammalian cell line to titer your lentiviral stocks as you will use to perform your expression studies (e.g., if you are performing expression studies in a dividing cell line or a non-primary cell line). If you have more than one lentiviral construct, we recommend that you titer all of the lentiviral constructs using the same mammalian cell line.