NeuroTrace™ Nissl 530/615红色荧光染色剂 - DMSO 溶液
NeuroTrace™ Nissl 530/615红色荧光染色剂 - DMSO 溶液
Invitrogen™

NeuroTrace™ Nissl 530/615红色荧光染色剂 - DMSO 溶液

尼氏染色是一种标准组织学方法,用于显示脑和脊髓中的神经元。尼氏物质由神经元核周体和树突中与粗面内质网相关的核糖体 RNA 组成,在损伤或再生神经元的细胞体内再分布,为神经元的生理状态提供标志物。NeuroTrace Nissl 530/615红色荧光染色剂对神经元特有的 Nissl 物质具有选择性,比甲苯胺蓝或甲酚紫等传统组织学染料更灵敏了解更多信息
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货号数量
N214821 mL
货号 N21482
价格(CNY)
3,929.00
Each
添加至购物车
数量:
1 mL
价格(CNY)
3,929.00
Each
添加至购物车
尼氏染色是一种标准组织学方法,用于显示脑和脊髓中的神经元。尼氏物质由神经元核周体和树突中与粗面内质网相关的核糖体 RNA 组成,在损伤或再生神经元的细胞体内再分布,为神经元的生理状态提供标志物。NeuroTrace Nissl 530/615红色荧光染色剂对神经元特有的 Nissl 物质具有选择性,比甲苯胺蓝或甲酚紫等传统组织学染料更灵敏。
仅供科研使用。不可用于诊断程序。
规格
颜色橙色
检测方法荧光
适用于(设备)荧光显微镜
产品类型尼氏染色
数量1 mL
运输条件室温
子细胞定位尼氏体
激发/发射530/615 nm
产品线NEUROTRACE
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

常见问题解答 (FAQ)

我可以使用NeuroTrace Nissl 染色剂染色胶质或其他类型细胞,该如何改善染色效果,使其对神经元的选择性更强?

我们的NeuroTrace Nissl 染色剂可标记Nissl底物——该底物由与粗面内质网相关的核糖体RNA构成,并大量存在于神经元中。这些染料并非完全特异性靶向神经元,但由于神经元蛋白质的合成水平高而对神经元选择性染色。在某些情况下它们可能会对其他类型细胞(如神经胶质细胞)染色,所以你可能需要降低染色浓度以获得更具选择性的神经元标记效果。对于神经元染色,我们建议的稀释范围是20到300倍。

NeuroTrace Nissl染色剂可染色石蜡切片吗?

我们只在小鼠脑冰冻切片上测试过,但是有文献指出NeuroTrace Nissl 染色剂可染色石蜡切片。

NeuroTrace Nissl 染色剂是什么?

这些染料是保密的,但它们是标记Nissl物质的染色剂。Nissl物质由与粗面内质网相关的核糖体RNA构成,并大量存在于神经元细胞中。 

你们有哪些神经元示踪产品?

详细信息请查阅此网页(https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html)。

I am labeling brain cryosections with a NeuroTrace Nissl Stain. Is this compatible with antibody labeling?

Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (21)

引用和文献
Abstract
Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury.
Authors:Bareyre FM, Kerschensteiner M, Misgeld T, Sanes JR
Journal:Nat Med
PubMed ID:16286922
'The rodent corticospinal tract (CST) has been used extensively to investigate regeneration and remodeling of central axons after injury. CST axons are currently visualized after injection of tracer dye, which is invasive, incomplete and prone to variation, and often does not show functionally crucial but numerically minor tract components. Here, ... More
A new method for imaging and 3D reconstruction of mammalian cochlea by fluorescent confocal microscopy.
Authors:Hardie NA, MacDonald G, Rubel EW
Journal:Brain Res
PubMed ID:15053969
'Traditional methods for anatomical and morphometric studies of cochlear tissues have relied upon either microdissection of the organ of Corti or the generation of serial sections of the cochlea. Such methods are time-consuming, disruptive to three-dimensional relationships and often restrict sampling to very limited numbers of cells. We have found ... More
Characterization of nestin expression in the spinal cord of GFP transgenic mice after peripheral nerve injury.
Authors:Matsumura S, Takagi K, Okuda-Ashitaka E, Lu J, Naritsuka H, Yamaguchi M, Ito S,
Journal:Neuroscience
PubMed ID:20673789
'Many studies have shown that activation and increase in the number of astrocytes and microglia in the spinal cord participate in the initiation and maintenance of neuropathic pain, but little attention has been paid to the responses of neural progenitor cells to peripheral nerve injury. Nestin, a class VI intermediate ... More
Synfire chains and cortical songs: temporal modules of cortical activity.
Authors:Ikegaya Y, Aaron G, Cossart R, Aronov D, Lampl I, Ferster D, Yuste R
Journal:Science
PubMed ID:15105494
How can neural activity propagate through cortical networks built with weak, stochastic synapses? We find precise repetitions of spontaneous patterns of synaptic inputs in neocortical neurons in vivo and in vitro. These patterns repeat after minutes, maintaining millisecond accuracy. Calcium imaging of slices reveals reactivation of sequences of cells during ... More
RNAi reveals doublecortin is required for radial migration in rat neocortex.
Authors:Bai J, Ramos RL, Ackman JB, Thomas AM, Lee RV, LoTurco JJ
Journal:Nat Neurosci
PubMed ID:14625554
Mutations in the doublecortin gene (DCX) in humans cause malformation of the cerebral neocortex. Paradoxically, genetic deletion of Dcx in mice does not cause neocortical malformation. We used electroporation of plasmids encoding short hairpin RNA to create interference (RNAi) of DCX protein in utero, and we show that DCX is ... More