I am labeling brain cryosections with a NeuroTrace Nissl Stain. Is this compatible with antibody labeling?
Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.
Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury.
Authors:Bareyre FM, Kerschensteiner M, Misgeld T, Sanes JR
Journal:Nat Med
PubMed ID:16286922
'The rodent corticospinal tract (CST) has been used extensively to investigate regeneration and remodeling of central axons after injury. CST axons are currently visualized after injection of tracer dye, which is invasive, incomplete and prone to variation, and often does not show functionally crucial but numerically minor tract components. Here, ... More
A new method for imaging and 3D reconstruction of mammalian cochlea by fluorescent confocal microscopy.
Authors:Hardie NA, MacDonald G, Rubel EW
Journal:Brain Res
PubMed ID:15053969
'Traditional methods for anatomical and morphometric studies of cochlear tissues have relied upon either microdissection of the organ of Corti or the generation of serial sections of the cochlea. Such methods are time-consuming, disruptive to three-dimensional relationships and often restrict sampling to very limited numbers of cells. We have found ... More
Characterization of nestin expression in the spinal cord of GFP transgenic mice after peripheral nerve injury.
Authors:Matsumura S, Takagi K, Okuda-Ashitaka E, Lu J, Naritsuka H, Yamaguchi M, Ito S,
Journal:Neuroscience
PubMed ID:20673789
'Many studies have shown that activation and increase in the number of astrocytes and microglia in the spinal cord participate in the initiation and maintenance of neuropathic pain, but little attention has been paid to the responses of neural progenitor cells to peripheral nerve injury. Nestin, a class VI intermediate ... More
Synfire chains and cortical songs: temporal modules of cortical activity.
Authors:Ikegaya Y, Aaron G, Cossart R, Aronov D, Lampl I, Ferster D, Yuste R
Journal:Science
PubMed ID:15105494
How can neural activity propagate through cortical networks built with weak, stochastic synapses? We find precise repetitions of spontaneous patterns of synaptic inputs in neocortical neurons in vivo and in vitro. These patterns repeat after minutes, maintaining millisecond accuracy. Calcium imaging of slices reveals reactivation of sequences of cells during ... More
RNAi reveals doublecortin is required for radial migration in rat neocortex.
Authors:Bai J, Ramos RL, Ackman JB, Thomas AM, Lee RV, LoTurco JJ
Journal:Nat Neurosci
PubMed ID:14625554
Mutations in the doublecortin gene (DCX) in humans cause malformation of the cerebral neocortex. Paradoxically, genetic deletion of Dcx in mice does not cause neocortical malformation. We used electroporation of plasmids encoding short hairpin RNA to create interference (RNAi) of DCX protein in utero, and we show that DCX is ... More