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Invitrogen
Rat IgG2b kappa Isotype Control (eB149/10H5), eBioscience™
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Rat IgG2b kappa Isotype Control (14-4031-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 20 µg/mL of Rat IgG2b K Isotype Control Purified (left) or 20 µg/mL of Anti-Mouse Granzyme B Purified (right) followed by 10 µg/mL of Anti-Rat IgG2b Biotin, Streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.
产品信息
14-4031-82
应用
建议稀释比
免疫组化（石蜡） (IHC (P))
Assay-Dependent
免疫细胞化学 (ICC/IF)
Assay-Dependent
流式细胞分析 (Flow)
Assay-Dependent
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Rat
/ IgG2b, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
eB149/10H5
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported: The monoclonal rat IgG2b kappa isotype control has been reported for use in flow cytometric analysis, immunohistochemistry, and immunocytochemistry.
Applications Tested: Purified Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
