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Neural Stem Cell Information
Find information, tools, and protocols for studying neural stem cells (NSCs). Explore this resource to enhance your experiments and help advance the field of neural stem cell biology.
What are neural stem cells?
Neural stem cells are a unique population of cells capable of self-renewal and differentiation into various neural cell types, including neurons, astrocytes, and oligodendrocytes. NSCs are typically made in specific regions of the brain, such as the subventricular zone (SVZ) and the hippocampus, where they reside in a niche that supports their growth and differentiation. Culturing NSCs in vitro allows researchers to study their properties, differentiation pathways, and potential therapeutic applications in neurodegenerative diseases and brain injuries.
To culture NSCs, researchers can isolate them from neural tissue and maintain them in a specialized growth medium containing essential nutrients and growth factors like EGF and FGF-2, which promote self-renewal and proliferation. NSCs can also be generated from pluripotent stem cells (PSCs) in vitro through a process called neural induction. Conventional methods of neural induction from PSCs include embryoid body (EB) formation and isolation of neural rosettes or co-cultures with stromal cell lines. These methods have several disadvantages including a time-consuming protocol and variability in the quality of resulting NSCs. Today, more efficient methods leverage small molecules and signaling pathways in a monolayer culture.
Why are neural stem cells important?
Neural stem cells are important because of their ability to self-renew and their multipotent capacity to differentiate into neurons and glial cells, which have essential functions in the nervous system. NSCs are not only a valuable source of cells for neuroscience research but also have potential as a regenerative medicine to help treat neurodegenerative diseases and other neurological disorders.
Generate functional and relevant cell models
Find online protocols, handbooks, and webinars to help ensure your research success with NSCs.
Neural stem cell resource
Gibco Neurobiology Protocol Handbook
This digital handbook contains our latest collection of over 23 optimized protocols, experimental best practices, and troubleshooting advice to support your neuroscience research. This protocol handbook covers topics of neural cell culture and differentiation, cell analysis, molecular characterization, and transfection. And includes a selected list of neuroscience publications and other resources to help you get started with your research.
Video: How to use Gibco PSC Neural Induction Medium
See how Gibco PSC Neural Induction Medium generates up to 20 million neural stem cells from pluripotent stem cells in just 7 days.
Selection guide: Neural cell culture products
Explore our product offering of neural stem cells, media and reagents designed to help build optimal cell models to advance your neurobiology research. Choose from our portfolio of products that support neural stem cell engineering, differentiation, and analysis.
StemPro NSC SFM is a robust serum-free medium for the growth and expansion of NSCs
- Excellent expansion efficiency
- Maintain normal NSC multipotency and phenotype/karyotype
- Versatility to support both adherent and suspension NSC cultures
- Better lot-to-lot consistency
Video: Improved differentiation of stem cell-derived neurons and downstream applications using CultureOne Supplement
Gibco CultureOne Supplement eliminates more than 75% of contaminating neural progenitor cells with minimal cell death and no effect on other kinase mediated pathways. Watch this video to see how CultureOne improves the differentiation of neural stem cells (NSCs) to neurons.
Stem Cell Research Resource Library
Access our collection of scientific application notes, protocols, videos, webinars, publications, posters, and more for stem cell research.
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