The revised Assay Development Technical Handbook is an essential resource for any laboratory using enzyme-linked immunosorbent assay (ELISA) and related plate-based assay methods. The handbook describes the essential techniques and tools for designing and optimizing ELISA Assays. Featured products include coated microplates, standards, blockers, buffers, probe-labeling reagents, secondary antibodies and detection substrates.

Contents include: Introduction to ELISA, Selecting an ELISA Plate, Thermo Scientific Pierce Microplates, Thermo Scientific Pierce Coated Microplates, Blocking and Washing, Blocking and Washing Reagents, Detection Probes, Antibody Labeling, Choosing a Substrate, Bulk and Custom Offerings, and Recommended Reading.

  Download the Assay Development Technical Handbook

Dialysis is a separation technique that gained popularity in life science laboratories during the 1950s. Research papers of that era described dialysis as a new, cutting-edge tool that scientists could use to unravel complex mixtures of biomacromolecules. Many of the dialysis theories established at that time are the cornerstones for contemporary products featured in this brochure. There are, however, two major differences between the dialysis tools of yesterday and today—preparation time and the amount of sample loss due to leaks. Early laboratory dialysis methods involved dedicating a significant amount of time to membrane preparation; Thermo Scientific Pierce Dialysis Products are essentially ready to use and resist sample leakage.

  Download the High-Performance Dialysis Brochure

Thermo Scientific Zeba 树脂技术可提供稳定的性能，让您高枕无忧。独特、专有的 Zeba 树脂能更好地回收蛋白质，即使是蛋白浓度较低的样品也不例外。

  下载Zeba 脱盐离心柱手册

The 40-page handbook reviews the principle of four major protein assay chemistries and contains an updated substance compatibility list. In addition, reaction schemes, protein-to-protein variation data, protocol schematics and a quick technical summary table accompany each assay methodology. The table includes the working range of the assay, characteristics and advantages, applications, disadvantages, and interfering substances. Useful references are provided with each assay.

  Download the Protein Assay Technical Handbook

我们的《阿维丁-生物素技术手册》共 48 页，汇集了生物素化、纯化或检测蛋白质所需的一切。特色产品包括细胞表面蛋白生物素化和纯化试剂盒、抗体标记和新型光反应生物素化试剂。本手册包括数十种参考文献以及操作规程、故障排除技巧、选择指南和可用工具的完整列表。

  阿维丁-生物素技术手册

The "Thermo Scientific Pierce Western Blotting Handbook and Troubleshooting Guide" (2014) details each step of the Western blotting process with technical information and products for transfer, blocking, washing, antibodies, substrates, film and stripping buffer, including Pierce Fast Western products. You will want to keep this booklet close at hand because it also includes protocols, references and a troubleshooting guide.

  Western Blotting Handbook and Troubleshooting Guide

我们的72页蛋白质相互作用技术手册提供了方案、技术和产品信息，以帮助最大限度地提高蛋白质相互作用研究的结果。该手册为免疫沉淀和共免疫沉淀测定、下拉测定、远蛋白印迹和交联提供了背景、有用的提示和故障排除建议。该手册还详细介绍了研究蛋白质-核酸相互作用的方法，包括ChIP、EMSA和RNA EMSA。该手册是任何研究蛋白质相互作用的实验室的重要资源。

内容包括：蛋白质相互作用介绍、共免疫沉淀试验、下拉试验、远西方印迹、蛋白质相互作用图谱、酵母双杂交报告物试验、电泳迁移率变化试验（EMSA）、染色质免疫沉淀试验（ChIP）、蛋白质-核酸偶联物等。

在这本 32 页的手册中，您可以了解更多有关如何使用各种 Thermo Scientific 蛋白生物学工具对蛋白质样品进行脱盐、缓冲液交换、浓缩和/或去除杂质、免疫沉淀以及其他蛋白质纯化和清理方法的信息。

• 免疫沉淀 (IP)、共同 IP 和染色质-IP
• 重组蛋白纯化标签
• 使用 Slide-A-Lyzer 透析盒和装置安全地透析蛋白质样品
• 使用 Zeba 自旋脱盐柱和板快速脱盐样品，提高蛋白质回收率
• 使用针对去除洗涤剂或内毒素而优化的树脂有效提取特定污染物
• 使用 Pierce 蛋白浓缩器快速浓缩稀释蛋白质样品

蛋白质制备手册 ›

Improve your mass spectrometry results
Explore the new mass spec digital resource center to get practical information and tips to help you achieve your goals. Access the site to gain access to these free resources:

• Downloadable Thermo Scientific Protein Sample Preparation and Quantitation for Mass Spectrometry Handbook about tools and techniques for more robust and reproducible sample processing, protein quantitation and instrument calibration
• Helpful white papers and late breaking posters on specific applications such as subcellular fractionation, peptide fractionation, isobaric labeling and more
• On-demand webinars covering protein quantitation methods

Mass Spectrometry Analysis Technical Handbook

Our Quantitative Proteomics Handbook provides an overview of protein quantitation tools and reagents for discovery and targeted proteomics.

  Quantitative Proteomics Handbook

Greg T. Hermanson 所著的《生物共轭技术》 （第 3 版，2013 年）是对这本被公认为生物共轭领域权威参考指南的书籍的重大更新。

《生物共轭技术》是一本完整的教科书和操作手册，可供希望学习和掌握生物分子交联、标记和固定化技术的生命科学家使用，这些技术是许多实验室应用的基础。对于希望为全新应用开发新型共轭策略的研究人员来说，本书也是一本详尽而可靠的参考书。书中还广泛介绍了生物共轭领域，涵盖了该技术在不同科学学科中的所有主要应用，以及为任何目的设计最佳生物共轭物的技巧。

生物共轭技术，第 3 版 ›

The updated Antibody Production and Purification Technical Handbook is an essential resource for any laboratory working with antibodies. The handbook provides an overview of antibody structure and types, as well as technical information on the procedures, reagents and tools used to produce, purify, fragment and label antibodies.

 Antibody Production and Purification Guide

This 45-page guide is of value to the novice as well as those who have previous experience with crosslinking reagents. It begins with a basic discussion on crosslinking and the reagents that are used. The guide also contains a discussion on various applications where crosslinking has been applied, including the powerful label transfer technique for identifying or confirming protein interactions. Crosslinking chemistry is addressed in an easy-to-follow format designed to convey the important information you need without getting lost in details. Each Thermo Scientific Pierce crosslinking reagent is shown along with its structure, molecular weight, spacer arm length and chemical reactivity. The handbook concludes with a list of excellent references on crosslinker use and a glossary of common crosslinking terms.

  Crosslinking Reagents Technical Handbook

The revised 80-page handbook provides technical and product information to help maximize results for protein purification procedures. The handbook provides background, helpful hints and troubleshooting advice for covalent coupling of affinity ligands to chromatography supports, avidin:biotin-binding, affinity purification of antibodies, immunoprecipitation and co-immunoprecipitation assays, affinity procedures for contaminant removal, and FPLC purification methods.

  Protein Purification Technical Handbook