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| Most common method Antibody binding | You have a biotinylated antibody towards the antigen Biotin binding | You have cell line with a recombinant (fusion tagged) target protein Recombinant protein binding | |
|---|---|---|---|
| Starting sample | Lysate from any cell/ bacteria/yeast, or body fluids | Lysate from any cell/ bacteria/yeast, or body fluids | Cell lines |
| Requires additional target antibody | Yes | Yes | No, beads are directly targeting the tagged proteins |
| Requires species specific antibodies | Yes | No | N/A |
| Antibody co-eluted off the beads | Yes/No (product specific) | Yes* | N/A |
| Notes |
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荧光成像初学者都会遇到很多问题。在荧光大课堂,您能了解荧光显微镜的基本知识，包括样品和培养基注意事项，样品制备和初学者实验方案，帮助您顺利入门。希望您能从Molecular Probes 荧光大课堂受益。
Resources for NativePAGE gels
Technical Resources
Resources for NativePAGE® gels
Additional resources for NuPAGE Precast Gel System
Resources for Novex Tricine gels
Technical Resources
Resources for Novex Tris-Glycine gels
Resources for Novex Tris-Glycine gels
Technical Resources
- NEW: Protein Electrophoresis and Western Blotting Support Center Find tips, troubleshooting help, and resources for your western workflow.
How to find a secondary antibody
Step 1: Search for the target species of interest (e.g., “anti-mouse” or “goat anti-rabbit” or “alexa fluor 488”).
Step 2: Narrow results by host species, conjugate, application, and other criteria using side filters.
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| By host species | By antibody isotype | By conjugate type |
|---|---|---|
| Goat | IgA | Unconjugated |
| Hamster | IgE | Alexa Fluor dyes |
| Human | IgG | Biotin conjugates |
| Mouse | IgG1 | Classic dyes (e.g. FITC, TRITC) |
| Rabbit | IgG2a | Pacific dyes |
| Rat | IgG2b | PE and APC |
| IgG3 | PerCP | |
| IgM | Qdot probe | |
| Multi | Tandems (e.g. PE-Alexa Fluor 647) | |
| TRI-COLOR |