Platinum™ SuperFi II Green PCR 预混液

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Platinum™ SuperFi II Green PCR 预混液

Name: Platinum™ SuperFi II Green PCR 预混液
SKU: 12369050
Price: 3626.10 CNY

Invitrogen Platinum SuperFi II 绿色 PCR 预混液 (2X) 是含有 Platinum了解更多信息

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货号	颜色	反应次数
12369050	Green	500 次反应
12369010	Green	100 次反应
12369250	Green	5 x 500 反应
12368010	Colorless	100 次反应
12368050	Colorless	500 次反应
12368250	Colorless	5 x 500 反应

6 选项

货号 12369050

价格（CNY)

3,626.10

飞享价

Ends: 31-Dec-2025

12,268.00

共减 8,641.90 (70%)

Each

添加至购物车

颜色:

Green

反应次数:

500 次反应

请求批量或定制报价

价格（CNY)

3,626.10

飞享价

Ends: 31-Dec-2025

12,268.00

共减 8,641.90 (70%)

Each

添加至购物车

Invitrogen Platinum SuperFi II 绿色 PCR 预混液 (2X) 是含有 Platinum SuperFi II DNA 聚合酶、Platinum SuperFi II 缓冲液和 dNTP 的即用型混合液，用于方便完成 PCR 配制，其还含有两种示踪染料，以便直接将 PCR 产物上样到凝胶上。Platinum SuperFi II DNA 聚合酶是一款校读 DNA 聚合酶，将超高保真度与创新性 SuperFi II 缓冲液结合在一起，可极大限度提高 PCR 在通用引物退火温度下的扩增成功率。它特别适用于需要高度序列准确性的克隆、诱变和其他应用。

Platinum SuperFi II Green PCR 预混液特点包括：
•卓越的 >300X Taq 保真度
•60°C 通用引物退火
• 出色的特异性、灵敏度和得率
•难扩增靶标（例如纯度欠佳的靶标、GC 含量 ˃65% 的靶标、长片段 PCR 需求）的可靠扩增

Platinum SuperFi II DNA 聚合酶是一种经过基因改造的酶，具有高持续合成能力，对 PCR 抑制剂具有更高的耐受性。它还匀许快速循环实验方案和扩增长靶标（长达 20 kb）。Platinum 热启动技术使用专有抗体，可在 PCR 变性步骤之前抑制酶活性，防止非特异性扩增和引物降解。该技术还可在室温下配制反应体系，提高了灵敏度和产量。

由于 SuperFi II PCR 缓冲液的独特成分，遵循一般设计规则设计的大多数引物对的退火温度均为 60°。缓冲液中的等稳定化分子可提高退火步骤中引物-模板的双重稳定性，并可增强特异性，而无需优化每个引物对的退火温度。使用 Platinum SuperFi II DNA 聚合酶，可以使用同一方案将不同的 PCR 测定共循环，该方案即使用通用引物退火温度和为待扩增的最长片段选择的延伸步骤。

Platinum SuperFi II DNA 多聚酶的应用：
•高保真 PCR
•克隆和亚克隆
•位点定向诱变
•富含 GC 的模板扩增
•用于测序的模板生成
•高通量 PCR
•扩增纯度不佳的样品
•长链 PCR
•快速 PCR

除此之外，我们还使用预混液Platinum SuperFi II PCR Master Mix。该预混液不同之处在于，不含有示踪染料。

查看有关 Platinum SuperFi II DNA 聚合酶产品的更多信息›

规格

颜色Green

保真度（相对于 Taq）>300X

热启动内置热启动

反应次数500 次反应

突出端平末端

聚合酶Platinum SuperFi II DNA 聚合酶

产品类型PCR Master Mix (2X)

数量500 reactions

反应形式SuperMix 或预混液

运输条件干冰

尺寸（最终产品）20 kb 或更小

最大浓度2X

适用于（应用）Hot-start PCR, High-fidelity PCR

高 GC PCR 扩增效果高

反应速度快速

Unit SizeEach

内容与储存

•10 x 1.25 mL Platinum SuperFi II Green PCR 预混液
•10 x 1.25 mL 无核酸酶水

足够用于500 50-μ L 反应

常见问题解答 (FAQ)

How much volume of the Platinum SuperFi II PCR Master Mix (2X) or Platinum SuperFi II PCR Green Master Mix (2X) should I use per reaction?

Typically, for a 50 µL PCR reaction, we recommend using 25µµL of the 2X master mix so that its final concentration in the reaction is 1X.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What is the best way to quantify your PCR samples using the Platinum SuperFi II PCR Master Mix, Green (Cat, No. 12369250, 12369010, 12369050)? Is it possible to quantify using Tapestation or a Qubit fluorometer?

The dyes in the Platinum SuperFi II Green PCR Master Mix can interfere with the fluorescent readings on both Qubit fluorometer and Tapestation. To accurately quantify your samples, you should first use a PCR purification kit, such as the GeneJet PCR Purification Kit (Cat. No. K0701), to clean up your samples. Alternatively, you can use the Platinum SuperFi II PCR Master Mix (Cat. No. 12368010) which does not contain the interfering dyes, thus eliminating the need for a purification step.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What are your recommendations when working with long amplicons using Platinum SuperFi II DNA Polymerase?

Good lab practices are important for long fragment amplification. These include using high-quality templates (pure, fresh, and intact) and fresh primer solutions. Reducing primer concentration to 0.2 µM may also improve the results.

Can I use Platinum SuperFi DNA Polymerase cycling protocol and primer annealing temperature with Platinum SuperFi II DNA Polymerase?

Yes, Platinum SuperFi II DNA Polymerase works at both universal 60 degrees C annealing temperature and at annealing temperatures calculated with a Tm calculator.

Can I perform TA cloning with Platinum SuperFi II DNA Polymerase?

Platinum SuperFi II DNA Polymerase produces blunt-ended PCR products that can be cloned directly into blunt-ended cloning vectors. TA cloning is also possible if 3' dA-overhangs are added after PCR. We recommend purifying the PCR products of Platinum SuperFi DNA Polymerase before adding the overhangs. The procedure for adding 3' dA-overhangs (TA cloning) includes the following steps:
- Purify the PCR product (e.g., with a PCR purification kit or phenol extraction/DNA precipitation). Before adding the overhangs, PCR product must be purified, as the strong proofreading activity of any remaining Platinum SuperFi DNA Polymerase will degrade the added 3' dA-overhangs.
- Perform 3' dA addition with a Taq DNA polymerase.
Reaction components:
Purified PCR product
0.2 mM dATP
1x Taq Buffer
1 U Taq DNA Polymerase
Incubate the reaction for 20 min at 72 degrees C.
- Proceed to TA cloning. For optimal ligation efficiency, we recommend using fresh PCR products, since 3' dA-overhangs can be lost during storage

View all Platinum™ SuperFi II Green PCR 预混液 FAQs