RIPA 裂解和提取缓冲液
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RIPA 裂解和提取缓冲液
引用和文献 (7)
Thermo Scientific™

RIPA 裂解和提取缓冲液

Thermo Scientific RIPA 裂解和提取缓冲液是一种用于培养的哺乳动物细胞的常用细胞裂解试剂的高质量、即用型且完全披露的配方。RIPA 缓冲液的特点：•便利— 即用型解决方案；无需自行装配和制备组成部分•灵活— 可与许多应用兼容，包括报告基因检测、蛋白检测了解更多信息
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货号数量
89900100 mL
89901250 mL
货号 89900
价格（CNY)
641.00
飞享价
Ends: 31-Dec-2025
867.00
共减 226.00 (26%)
Each
添加至购物车
数量:
100 mL
请求批量或定制报价
价格（CNY)
641.00
飞享价
Ends: 31-Dec-2025
867.00
共减 226.00 (26%)
Each
添加至购物车
Thermo Scientific RIPA 裂解和提取缓冲液是一种用于培养的哺乳动物细胞的常用细胞裂解试剂的高质量、即用型且完全披露的配方。

RIPA 缓冲液的特点：

•便利— 即用型解决方案；无需自行装配和制备组成部分
•灵活— 可与许多应用兼容，包括报告基因检测、蛋白检测、免疫检测和蛋白纯化
•通用— 可提取细胞质、细胞膜和细胞核蛋白
•披露的配方— 不含专有组成部分，用户可全面控制和了解可能存在的兼容性问题

该 RIPA 缓冲液可从培养的哺乳动物细胞（包括铺板细胞和团状悬浮细胞）中高效裂解并提取蛋白。该常用试剂可提取细胞膜、细胞核和细胞质蛋白，并可与许多应用兼容，包括报告基因检测、Thermo Scientific BCA 蛋白检测、免疫检测和蛋白纯化。Thermo Scientific Halt 蛋白酶抑制剂混合物（产品编号 78430）和 Halt 磷酸酶抑制剂混合物（产品编号 78420）等抑制剂也可与该 RIPA 缓冲液配方兼容，可在使用前添加以防止蛋白水解并保持蛋白磷酸化。

RIPA 缓冲液的名称来源于为其开发这种缓冲液的原始应用：放射免疫沉淀测定。尽管目前在实验室中很少使用这种同位素检测方法，但该裂解缓冲液配方的首字母缩略词已被广泛使用。RIPA 细胞裂解试剂可从各种细胞类型中高效提取蛋白，因为其含有三种非离子型和离子型去污剂。该去污剂配方的一大劣势是，与其他裂解试剂相比，其与某些下游应用相对不兼容。

相关产品
Pierce™ IP 裂解缓冲液
M-PER™ 哺乳动物蛋白提取试剂
仅供科研使用。不可用于诊断程序。
规格
产品规格液体
数量100 mL
容积（公制）100 mL
产品类型提取缓冲液
Unit SizeEach
内容与储存
接收后，在 4°C 下储存。

常见问题解答 (FAQ)

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Does RIPA Lysis and Extraction Buffer (Cat. No. 89900, 89901) contain protease or phosphatase inhibitors?

RIPA Lysis and Extraction Buffer (Cat. No. 89900, 89901) does not contain protease or phosphatase inhibitors. If desired, you may add protease and/or phosphatase inhibitors, such as Halt Protease Inhibitor Cocktail (Cat. No. 78410) and Halt Phosphatase Inhibitor Cocktail (Cat. No. 78420) to the RIPA Lysis and Extraction Buffer to prevent proteolysis and maintain phosphorylation status of proteins. We recommend adding protease and phosphatase inhibitors immediately before use.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why is it not recommended that I use RIPA buffer for protein A280 measurements with my NanoDrop spectrophotometer?

RIPA buffer produces a particularly strong absorbance signal at the 280 nm wavelength. As a result, it will either over estimate or under estimate protein concentrations and interfere with the protein purity ratio.
Protein samples in RIPA buffer should be quantified via the Pierce Protein 660 or BCA colorimetric assays using a full spectrum NanoDrop model.

Find additional tips, troubleshooting help, and resources within ourProtein Purification and Isolation Support Center.

Why is there low phosphorylation of the proteins when I use the RIPA Lysis and Extraction Buffer?

Low phosphorylation is usually due to phosphatase activity. We recommend adding a Halt Phosphatase Inhibitor Cocktail to the buffer before use.
Alternatively, the protein is not phosphorylated or phosphorylated at a low level.

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

What if proteolysis occurs when I use RIPA Lysis and Extraction Buffer?

Proteolysis indicates that no protease inhibitors were added. We recommend adding a Halt Protease Inhibitor Cocktail to the RIPA Lysis and Extraction Buffer before use.

Find additional tips, troubleshooting help, and resources within ourProtein Purification and Isolation Support Center.

View all RIPA 裂解和提取缓冲液 FAQs

引用和文献 (7)

引用和文献
Abstract
Shatavari supplementation in postmenopausal women alters the skeletal muscle proteome and pathways involved in training adaptation.
Authors:O'Leary MF,Jackman SR,Bowtell JL
Journal:European journal of nutrition
PubMed ID:38214710
PURPOSE: Shatavari is an understudied, widely available herbal supplement. It contains steroidal saponins and phytoestrogens. We previously showed that six weeks of shatavari supplementation improved handgrip strength and increased markers of myosin contractile function. Mechanistic insights into shatavari's actions are limited. Therefore, we performed proteomics on vastus lateralis (VL) samples ... More
Cerebral microvascular endothelial cell-derived extracellular vesicles regulate blood - brain barrier function.
Authors:Hosseinkhani B,Duran G,Hoeks C,Hermans D,Schepers M,Baeten P,Poelmans J,Coenen B,Bekar K,Pintelon I,Timmermans JP,Vanmierlo T,Michiels L,Hellings N,Broux B
Journal:Fluids and barriers of the CNS
PubMed ID:38114994
Autoreactive T lymphocytes crossing the blood-brain barrier (BBB) into the central nervous system (CNS) play a crucial role in the initiation of demyelination and neurodegeneration in multiple sclerosis (MS). Recently, extracellular vesicles (EV) secreted by BBB endothelial cells (BBB-EC) have emerged as a unique form of cell-to-cell communication that contributes ... More
Optimization of a Protocol for Protein Extraction from Calcified Aortic Valves for Proteomics Applications: Development of a Standard Operating Procedure.
Authors:Trindade F,Ferreira AF,Saraiva F,Martins D,Mendes VM,Sousa C,Gavina C,Leite-Moreira A,Manadas B,Falcão-Pires I,Vitorino R
Journal:Proteomes
PubMed ID:36136308
The comprehension of the pathophysiological mechanisms, the identification of druggable targets, and putative biomarkers for aortic valve stenosis can be pursued through holistic approaches such as proteomics. However, tissue homogenization and protein extraction are made difficult by tissue calcification. The reproducibility of proteome studies is key in clinical translation of ... More
TGFB1-induced extracellular expression of TGFBIp and inhibition of TGFBIp expression by RNA interference in a human corneal epithelial cell line.
Authors:Yellore VS, Rayner SA, Aldave AJ
Journal:Invest Ophthalmol Vis Sci
PubMed ID:20881301
'To report the increased production of extracellular transforming growth factor ß-induced protein (TGFBIp) by human corneal epithelial cells (HCECs) after induction by TGFB1 and the inhibition of TGFBIp production in induced and noninduced HCECs by RNA interference (RNAi).' ... More
Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs.
Authors:Pei M, He F, Boyce BM, Kish VL
Journal:Osteoarthritis Cartilage
PubMed ID:19128988
Synovium-derived stem cells (SDSCs) have proven to be superior in cartilage regeneration compared with other sources of mesenchymal stem cells. We hypothesized that conventionally passaged SDSCs can be engineered in vitro into cartilage tissue constructs and the engineered premature tissue can be implanted to repair allogeneic full-thickness femoral condyle cartilage ... More
View all RIPA 裂解和提取缓冲液 citations