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反转录酶(RT)以 RNA 为模板合成互补 DNA(cDNA),可用于 RT-qPCR、RT-PCR、RNA 测序和基因表达分析等应用。您可以选择反转录单酶、预混液以及 cDNA 合成试剂盒,满足常规样本、挑战性样本、低起始量样本和高通量反转录实验流程的需求。
Invitrogen SuperScript V 产品系列是新一代 cDNA 合成解决方案,兼具出色性能、便捷操作和卓越稳定性。SuperScript V 产品旨在为多种类型的样本提供出色的灵敏度和稳健性能,包括 GC 含量高、结构复杂、降解以及含抑制剂的 RNA。此外,该系列产品还带来了实验流程上的创新,包括快速的反应时间、配制后反应体系的室温稳定性,以及灵活的引物选择,让您在实验中拥有更高的灵活性和控制力。其稳定性特性还支持室温运输、储存和反应体系配制。
除核心的反转录单酶和预混液形式外,SuperScript 产品组合还包括针对特殊 cDNA 合成而优化的特殊试剂盒。这些解决方案可将 SuperScript 的性能延伸至一步法 RT-PCR、低起始量样本预扩增、模板转换和直接 cDNA 合成等应用。
这些试剂盒可简化实验流程,减少移液步骤,从而降低操作失误和污染风险,并适用于高通量应用。
此外,我们还提供多种反转录解决方案,可满足专业应用、常规实验以及注重成本效益的不同工作流程需求。
Choose a standalone reverse transcriptase when you want maximum flexibility to select primers and optimize reaction conditions.
Choose a master mix when you want a streamlined workflow with fewer setup steps; options are available with optimized primers or without primers for greater priming flexibility.
Choose a kit when you want an optimized solution for simplified or high-throughput workflows.
Consider a high-performance reverse transcriptase such as SuperScript V Reverse Transcriptase designed to work across challenging sample types including GC-rich, structured, degraded, and inhibitor-rich RNA and can detect as low as 100 fg input RNA.
For very low-input or single-cell samples, consider solutions specifically designed for preamplification.
For routine two-step RT-qPCR, a ready-to-use master mix can simplify reaction setup. SuperScript V VILO Master Mix contains optimized oligo (dT) and random primers, while the primer-free format provides the convenience of a master mix with flexibility to choose the priming strategy.
Choose a one-step RT-PCR kit when you want reverse transcription and amplification to occur in a single workflow.
Direct reverse transcription products are designed to eliminate a separate RNA-isolation step by combining cell lysis and cDNA synthesis in a single tube.
Our value brand products are manufactured to the highest quality standards, widely viewed as premium brands and well cited in publications.
| Premium performance: SuperScript V products | Enhanced value: Maxima H Minus products | Routine economy: High Capacity products |
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仅限科研用途。不得用于诊断程序。